1887

Abstract

A novel virus, named (ATCV), that infects endosymbiotic algae of the heliozoon was isolated from freshwater samples. Electron microscopic analysis of ATCV revealed that the viral capsid has a distinct icosahedral shape with a diameter of 140–190 nm. Filamentous structures extending from some of the virus vertices, which may aid attachment of the virus to host cells, were also observed. The capsid is made up of one major coat protein of about 50 kDa and contains a large dsDNA genome. ATCV is a member of the genus , which belongs to the family , a group of large, icosahedral, dsDNA-containing viruses that infect algae and are ubiquitous in natural environments. However, ATCV is clearly distinct from the prototype , (PBCV-1), in some aspects of its genome structure and gene content and therefore must be regarded as a member of a new group of viruses.

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2005-10-01
2019-10-15
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References

  1. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A. & Struhl, K. ( 1987; ). Current Protocols in Molecular Biology. New York: Wiley.
  2. Chen, F. & Suttle, C. A. ( 1995; ). Amplification of DNA polymerase gene fragments from viruses infecting microalgae. Appl Environ Microbiol 61, 1274–1278.
    [Google Scholar]
  3. Chen, F. & Suttle, C. A. ( 1996; ). Evolutionary relationships among large double-stranded DNA viruses that infect microalgae and other organisms as inferred from DNA polymerase genes. Virology 219, 170–178.[CrossRef]
    [Google Scholar]
  4. Iyer, L. M., Aravind, L. & Koonin, E. V. ( 2001; ). Common origin of four diverse families of large eukaryotic DNA viruses. J Virol 75, 11720–11734.[CrossRef]
    [Google Scholar]
  5. Laemmli, U. K. ( 1970; ). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.[CrossRef]
    [Google Scholar]
  6. Plugge, B., Becker, B. & Wolf, A. H. ( 1999; ). Several genes in Chlorella virus strain CVG-1 encode putative virion components. J Gen Virol 80, 1067–1072.
    [Google Scholar]
  7. Reisser, W., Burbank, D. E., Meints, S. M., Meints, R. H., Becker, B. & Van Etten, J. L. ( 1988; ). A comparison of viruses infecting two different Chlorella-like green algae. Virology 167, 143–149.[CrossRef]
    [Google Scholar]
  8. Rohozinski, J., Girton, L. E. & Van Etten, J. L. ( 1989; ). Chlorella viruses contain linear nonpermuted double-stranded DNA genomes with covalently closed hairpin ends. Virology 168, 363–369.[CrossRef]
    [Google Scholar]
  9. Sambrook, J., Fritsch, E. F. & Maniatis, T. ( 1989; ). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  10. Sanger, F., Nicklen, S. & Coulson, A. R. ( 1977; ). DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A 74, 5463–5467.[CrossRef]
    [Google Scholar]
  11. Van Etten, J. L. ( 2003; ). Unusual life style of giant chlorella viruses. Annu Rev Genet 37, 153–195.[CrossRef]
    [Google Scholar]
  12. Van Etten, J. L. & Meints, R. H. ( 1999; ). Giant viruses infecting algae. Annu Rev Microbiol 53, 447–494.[CrossRef]
    [Google Scholar]
  13. Van Etten, J. L., Burbank, D. E., Kuczmarski, D. & Meints, R. H. ( 1983; ). Virus infection of culturable Chlorella-like algae and development of a plaque-assay. Science 219, 994–996.[CrossRef]
    [Google Scholar]
  14. Van Etten, J. L., Lane, L. C. & Meints, R. H. ( 1991; ). Viruses and virus-like particles of eukaryotic algae. Microbiol Rev 55, 586–620.
    [Google Scholar]
  15. Wang, I.-N., Li, Y., Que, Q., Bhattacharya, M., Lane, L. C., Chaney, W. G. & Van Etten, J. L. ( 1993; ). Evidence for virus-encoded glycosylation specificity. Proc Natl Acad Sci U S A 90, 3840–3844.[CrossRef]
    [Google Scholar]
  16. Wilson, W. H., Van Etten, J. L., Schroeder, D. S., Nagasaki, K., Brussaard, C., Delaroque, N., Bratbak, G. & Suttle, C. ( 2005; ). Phycodnaviridae. In Virus Taxonomy, VIIIth Report of the ICTV, pp. 163–175. Edited by C. M. Fauquet, M. A. Mayo, J. Maniloff, U. Desselberger & L. A. Ball. London: Elsevier/Academic Press.
  17. Yamada, T. & Higahiyama, T. ( 1993; ). Characterization of the terminal inverted repeats and their neighboring tandem repeats in the Chlorella CVK1 virus genome. Mol Gen Genet 241, 554–563.
    [Google Scholar]
  18. Yan, X., Olson, N. H., Van Etten, J. L., Bergoin, M., Rossmann, M. G. & Baker, T. S. ( 2000; ). Structure and assembly of large lipid-containing dsDNA viruses. Nat Struct Biol 7, 101–103.[CrossRef]
    [Google Scholar]
  19. Zhang, Y., Strasser, P., Grabherr, R. & Van Etten, J. L. ( 1994; ). Hairpin loop structure at the termini of the chlorella virus PBCV-1 genome. Virology 202, 1079–1082.[CrossRef]
    [Google Scholar]
  20. Zhang, Y., Adams, B., Sun, L., Burbank, D. E. & Van Etten, J. L. ( 2001; ). Intron conservation in the DNA polymerase gene encoded by Chlorella viruses. Virology 285, 313–321.[CrossRef]
    [Google Scholar]
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