1887

Abstract

(SDV) is an emerging pathogen in salmonid aquacultures, the impact of which is underestimated to date due to the lack of efficient diagnostic tools. To better characterize this new aquatic alphavirus and to make molecular tools available, a panel of monoclonal antibodies (mAbs) directed against SDV non-structural and structural proteins has been generated by immunizing mice with SDV-specific recombinant proteins overexpressed in as antigens. So far, mAbs against nsP1, nsP3, E2 and E1 SDV proteins have been produced and their reactivity has been characterized by Western blot, radioimmunoprecipitation and indirect immunofluorescence assays. In addition, protein domains recognized by each mAb have been determined by immunofluorescence assay on truncated expressed SDV-derived proteins. Finally, one mAb directed against the E1 glycoprotein has been evaluated as a potential tool to be used in immunohistochemistry assay on experimentally infected trout.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.81030-0
2005-11-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/86/11/3119.html?itemId=/content/journal/jgv/10.1099/vir.0.81030-0&mimeType=html&fmt=ahah

References

  1. Acheson N. H., Tamm I. 1967; Replication of Semliki Forest virus: an electron microscopic study. Virology 32:128–143 [CrossRef]
    [Google Scholar]
  2. Ahola T., Lampio A., Auvinen P., Kaariainen L. 1999; Semliki Forest virus mRNA capping enzyme requires association with anionic membrane phospholipids for activity. EMBO J 18:3164–3172 [CrossRef]
    [Google Scholar]
  3. Anderson P. N., Potter M. 1969; Induction of plasma cell tumors in BALB-c mice with 2,6,10,14-tetramethylpentadecane (pristane). Nature 222:994–995 [CrossRef]
    [Google Scholar]
  4. Anonymous. 1985; Maladie du sommeil et pollutions expérimentales. Rapport technique n°118, Laboratoire National de Pathologie des Animaux Aquatiques.
  5. Boucher P. 1995; Etiologie et pathogénie de la maladie du pancréas et de la maladie du sommeil chez les salmonidés d'élevage . PhD thesis Université de Rennes I; Rennes, France:
  6. Boucher P., Baudin-Laurencin F. 1994; Sleeping disease of salmonids. Bull Eur Assoc Fish Pathol 14:179–180
    [Google Scholar]
  7. Boucher P., Baudin-Laurencin F. 1996; Sleeping disease and pancreas disease: comparative histopathology and acquired cross-protection. J Fish Dis 19:303–310 [CrossRef]
    [Google Scholar]
  8. Boucher P., Castric J., Baudin-Laurencin F. 1994; Observation of virus-like particles in rainbow-trout Oncorhynchus mykiss infected with sleeping disease virulent material. Bull Eur Assoc Fish Pathol 14:215–216
    [Google Scholar]
  9. Castric J., Baudin-Laurencin F., Bremont M., Jeffroy J., Le Ven A., Bearzotti M. 1997; Isolation of the virus responsible for sleeping-disease in experimentally infected rainbow-trout ( Oncorhynchus mykiss ). Bull Eur Assoc Fish Pathol 17:27–30
    [Google Scholar]
  10. Fijan N., Sulimanovic D., Bearzotti M., Muzinic D., Zwillenberg L. O., Chilmonczyk S., Vautherot J. F., de Kinkelin P. 1983; Some properties of the epithelioma papulosum cyprini (EPC) cell line from carp Cyprinus carpio . Ann Inst Pasteur Virol 134E:207–220
    [Google Scholar]
  11. Froshauer S., Kartenbeck J., Helenius A. 1988; Alphavirus RNA replicase is located on the cytoplasmic surface of endosomes and lysosomes. J Cell Biol 107:2075–2086 [CrossRef]
    [Google Scholar]
  12. Fuerst T. R., Niles E. G., Studier F. W., Moss B. 1986; Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase. Proc Natl Acad Sci U S A 83:8122–8126 [CrossRef]
    [Google Scholar]
  13. Grimley P. M., Berezesky I. K., Friedman R. M. 1968; Cytoplasmic structures associated with an arbovirus infection: loci of viral ribonucleic acid synthesis. J Virol 2:1326–1338
    [Google Scholar]
  14. Lannan C. N., Winton J. R., Fryer J. L. 1984; Fish cell lines: establishment and characterization of nine cell lines from salmonids. In Vitro 20:671–676 [CrossRef]
    [Google Scholar]
  15. Levy-Mintz P., Kielian M. 1991; Mutagenesis of the putative fusion domain of the Semliki Forest virus spike protein. J Virol 65:4292–4300
    [Google Scholar]
  16. Munro A. L. S., Ellis A. E., McVicar A. H., McLay H. A. 1984; An exocrine pancreas disease of farmed Atlantic Salmon in Scotland. Helgol Wiss Meeresunters 37:571–586
    [Google Scholar]
  17. Peranen J. 1991; Localization and phosphorylation of Semliki Forest virus non-structural protein nsP3 expressed in COS cells from a cloned cDNA. J Gen Virol 72:195–199 [CrossRef]
    [Google Scholar]
  18. Peranen J., Kaariainen L. 1991; Biogenesis of type I cytopathic vacuoles in Semliki Forest virus-infected BHK cells. J Virol 65:1623–1627
    [Google Scholar]
  19. Peranen J., Laakkonen P., Hyvonen M., Kaariainen L. 1995; The alphavirus replicase protein nsP1 is membrane-associated and has affinity to endocytic organelles. Virology 208:610–620 [CrossRef]
    [Google Scholar]
  20. Rumenapf T., Strauss E. G., Strauss J. H. 1995; Aura virus is a New World representative of Sindbis-like viruses. Virology 208:621–633 [CrossRef]
    [Google Scholar]
  21. Schenk P. M., Baumann S., Mattes R., Steinbiss H. H. 1995; Improved high-level expression system for eukaryotic genes in Escherichia coli using T7 RNA polymerase and rare ArgtRNAs. Biotechniques 19:196–198
    [Google Scholar]
  22. Tellinghuisen T. L., Hamburger A. E., Fisher B. R., Ostendorp R., Kuhn R. J. 1999; In vitro assembly of alphavirus cores by using nucleocapsid protein expressed in Escherichia coli . J Virol 73:5309–5319
    [Google Scholar]
  23. Villoing S. 2000; Le virus responsable de la maladie du sommeil chez les salmonidés: un nouveau type d'alphavirus . PhD thesis Université de Paris XI; Orsay, France:
  24. Villoing S., Bearzotti M., Chilmonczyk S., Castric J., Bremont M. 2000; Rainbow trout sleeping disease virus is an atypical alphavirus. J Virol 74:173–183 [CrossRef]
    [Google Scholar]
  25. Weston J., Villoing S., Bremont M. 8 other authors 2002; Comparison of two aquatic alphaviruses, salmon pancreas disease virus and sleeping disease virus, by using genome sequence analysis, monoclonal reactivity, and cross-infection. J Virol 76:6155–6163 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.81030-0
Loading
/content/journal/jgv/10.1099/vir.0.81030-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error