%0 Journal Article %A van Oers, Monique M. %A Abma-Henkens, Marleen H. C. %A Herniou, Elisabeth A. %A de Groot, Joost C. W. %A Peters, Sander %A Vlak, Just M. %T Genome sequence of Chrysodeixis chalcites nucleopolyhedrovirus, a baculovirus with two DNA photolyase genes %D 2005 %J Journal of General Virology, %V 86 %N 7 %P 2069-2080 %@ 1465-2099 %R https://doi.org/10.1099/vir.0.80964-0 %I Microbiology Society, %X The complete genome sequence of a single nucleocapsid nucleopolyhedrovirus recently isolated from Chrysodeixis chalcites (ChchNPV) was determined. The viral genome has a size of 149 622 bp and an overall G+C content of 39·1 mol%. The sequence contains 151 predicted open reading frames (ORFs) with a minimal size of 50 codons. The similarity of these ORFs with those of other completely sequenced baculoviruses was calculated using a newly developed database, named gecco. Phylogenetic analysis of the whole genome confirmed the evolutionary relationship of ChchNPV with group II NPVs, as did the absence of the NPV group I-specific gp64 gene. It is the first group II NPV to encode proliferating cell nuclear antigen. Most noteworthy is the presence of two ORFs encoding a class II cyclobutane pyrimidine dimer DNA photolyase. These two ORFs share only 45 % amino acid identity and have different promoter motifs. Twenty-two additional unique baculovirus genes were identified, including a gene encoding a novel putative RING finger protein with a possible homologue in poxviruses. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.80964-0