1887

Abstract

Viral molecular determinant(s) involved in the tobacco vein necrosis (TVN) symptom induced by necrotic isolates of (PVY) on cv. Xanthi leaves remain undetermined. Reference isolates belonging to PVY (infectious PVY-605 clone) and PVY (PVY-139) were used to produce PVY chimeric genomes by using reverse-genetic techniques. These chimeric clones were inoculated biolistically onto plants to establish the clone, prior to being tested on for their ability to induce TVN symptoms. Comparison between sequence data and symptoms observed for each mutated PVY construct shows that the C-terminal part of the multifunctional HC-Pro protein includes two residues (K and E) that are involved in TVN induced by PVY isolates. Site-directed mutagenesis was used to confirm that these two HC-Pro residues are involved in the TVN phenotype.

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2005-07-01
2024-04-19
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References

  1. Anandalakshmi R., Pruss G. J., Ge X., Marathe R., Mallory A. C., Smith T. H., Vance V. B. 1998; A viral suppressor of gene silencing in plants. Proc Natl Acad Sci U S A 95:13079–13084 [CrossRef]
    [Google Scholar]
  2. Atreya C. D., Atreya P. L., Thornbury D. W., Pirone T. P. 1992; Site-directed mutations in the potyvirus HC-PRO gene affect helper component activity, virus accumulation, and symptom expression in infected tobacco plants. Virology 191:106–111 [CrossRef]
    [Google Scholar]
  3. Brantley J. D., Hunt A. G. 1993; The N-terminal protein of the polyprotein encoded by the potyvirus tobacco vein mottling virus is an RNA-binding protein. J Gen Virol 74:1157–1162 [CrossRef]
    [Google Scholar]
  4. Brunt A. A., Crabtree K., Dallwitz M. J., Gibbs A. J., Watson L., Zurcher E. J. 1996; Plant Viruses Online: Descriptions and Lists from the VIDE Database . Version: 20 August 1996 http://image.fs.uidaho.edu/vide/
  5. Carrington J. C., Cary S. M., Parks T. D., Dougherty W. G. 1989; A second proteinase encoded by a plant potyvirus genome. EMBO J 8:365–370
    [Google Scholar]
  6. Cecchini E., Gong Z., Geri C., Covey S. N., Milner J. J. 1997; Transgenic Arabidopsis lines expressing gene VI from cauliflower mosaic virus variants exhibit a range of symptom-like phenotypes and accumulate inclusion bodies. Mol Plant Microbe Interact 10:1094–1101 [CrossRef]
    [Google Scholar]
  7. De Bokx J. A., Huttinga H. 1981 Potato virus Y (CMI/AAB Descriptions of Plant Viruses no. 242 Kew, UK: Commonwealth Microbiology Institute and Association of Applied Biology;
    [Google Scholar]
  8. Dougherty W. G., Carrington J. C. 1988; Expression and function of potyviral gene products. Annu Rev Phytopathol 26:123–143 [CrossRef]
    [Google Scholar]
  9. Glais L., Tribodet M., Kerlan C. 2002; Genomic variability in potato potyvirus Y PVY: evidence that PVYNW and PVYNTN variants are single to multiple recombinants between PVYO and PVYN isolates. Arch Virol 147:363–378 [CrossRef]
    [Google Scholar]
  10. Hirata H., Lu X., Yamaji Y., Kagiwada S., Ugaki M., Namba S. 2003; A single silent substitution in the genome of Apple stem grooving virus causes symptom attenuation. J Gen Virol 84:2579–2583 [CrossRef]
    [Google Scholar]
  11. Jakab G., Droz E., Brigneti G., Baulcombe D., Malnoë P. 1997; Infectious in vivo and in vitro transcripts from a full-length cDNA clone of PVY-N605, a Swiss necrotic isolate of potato virus Y. J Gen Virol 78:3141–3145
    [Google Scholar]
  12. Jenner C. E., Wang X., Tomimura K., Ohshima K., Ponz F., Walsh J. A. 2003; The dual role of the potyvirus P3 protein of Turnip mosaic virus as a symptom and avirulence determinant in brassicas. Mol Plant Microbe Interact 16:777–784 [CrossRef]
    [Google Scholar]
  13. Kasschau K. D., Cronin S., Carrington J. C. 1997; Genome amplification and long-distance movement functions associated with the central domain of tobacco etch potyvirus helper component–proteinase. Virology 228:251–262 [CrossRef]
    [Google Scholar]
  14. Ke S.-H., Madison E. L. 1997; Rapid and efficient site-directed mutagenesis by single-tube ‘megaprimer’ PCR method. Nucleic Acids Res 25:3371–3372 [CrossRef]
    [Google Scholar]
  15. Kong Q., Oh J.-W., Simon A. E. 1995; Symptom attenuation by a normally virulent satellite RNA of turnip crinkle virus is associated with the coat protein open reading frame. Plant Cell 7:1625–1634 [CrossRef]
    [Google Scholar]
  16. Le Romancer M., Kerlan C., Nedellec M. 1994; Biological characterization of various geographical isolates of potato virus Y inducing superficial necrosis on potato tubers. Plant Pathol 43:138–144 [CrossRef]
    [Google Scholar]
  17. Maia I. G., Haenni A.-L., Bernardi F. 1996; Potyviral HC-Pro: a multifunctional protein. J Gen Virol 77:1335–1341 [CrossRef]
    [Google Scholar]
  18. Oh C.-S., Carrington J. C. 1989; Identification of essential residues in potyvirus proteinase HC-Pro by site-directed mutagenesis. Virology 173:692–699 [CrossRef]
    [Google Scholar]
  19. Rigden J. E., Krake L. R., Rezaian M. A., Dry I. B. 1994; ORF C4 of tomato leaf curl geminivirus is a determinant of symptom severity. Virology 204:847–850 [CrossRef]
    [Google Scholar]
  20. Rodríguez-Cerezo E., Ammar E. D., Pirone T. P., Shaw J. G. 1993; Association of the non-structural P3 viral protein with cylindral inclusions in potyvirus-infected cells. J Gen Virol 74:1945–1949 [CrossRef]
    [Google Scholar]
  21. Saénz P., Cervera M. T., Dallot S., Quiot L., Quiot J.-B., Riechmann J. L., García J. A. 2000; Identification of a pathogenicity determinant of Plum pox virus in the sequence encoding the C-terminal region of protein P3+6K(1. J Gen Virol 81:557–566
    [Google Scholar]
  22. Shukla D. D., Ward C. W., Brunt A. A. 1994 The Potyviridae Wallingford, UK: CAB International;
    [Google Scholar]
  23. Sigvald R. 1984; The relative efficiency of some aphid species as vector of potato virus Y. Potato Res 27:285–290 [CrossRef]
    [Google Scholar]
  24. van der Vossen E. A. G., Neeleman L., Bol J. F. 1996; The 5′ terminal sequence of alfalfa mosaic virus RNA 3 is dispensable for replication and contains a determinant for symptom formation. Virology 221:271–280 [CrossRef]
    [Google Scholar]
  25. Verchot J., Carrington J. C. 1995; Evidence that the potyvirus P1 proteinase functions in trans as an accessory factor for genome amplification. J Virol 69:3668–3674
    [Google Scholar]
  26. Zhang L., Hanada K., Palukaitis P. 1994; Mapping local and systemic symptom determinants of cucumber mosaic cucumovirus in tobacco. J Gen Virol 75:3185–3191 [CrossRef]
    [Google Scholar]
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