A novel envelope protein involved in infection Free

Abstract

One open reading frame (designated ) from the (WSSV) genome has the motif of a cytokine I receptor and has been identified as a structural protein. In this paper, was expressed in and used to prepare a specific antibody to determine the location of the corresponding protein in the intact virion, the nucleocapsids and the envelope of WSSV. Western blotting with the VP76 antiserum confirmed that VP76 was an envelope protein of WSSV. To investigate the function of the VP76, WSSV was neutralized with the VP76-specific antiserum at different concentrations and injected intramuscularly into crayfish. The mortality curves showed that the VP76 antiserum could partially attenuate infection with WSSV, suggesting that VP76 is an envelope protein involved in WSSV infection.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.80923-0
2005-05-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/86/5/vir861357.html?itemId=/content/journal/jgv/10.1099/vir.0.80923-0&mimeType=html&fmt=ahah

References

  1. Barry M., McFadden G. 1997; Virus encoded cytokines and cytokine receptors. Parasitology 115:S89–S100 [CrossRef]
    [Google Scholar]
  2. Chang P.-S., Chen H.-C., Wang Y.-C. 1998; Detection of white spot syndrome associated baculovirus in experimentally infected wild shrimp, crab and lobsters by in situ hybridization. Aquaculture 164:233–242 [CrossRef]
    [Google Scholar]
  3. Chen L.-L., Leu J.-H., Huang C.-J., Chou C.-M., Chen S.-M., Wang C.-H., Lo C.-F., Kou G.-H. 2002a; Identification of a nucleocapsid protein (VP35) gene of shrimp white spot syndrome virus and characterization of the motif important for targeting VP35 to the nuclei of transfected insect cells. Virology 293:44–53 [CrossRef]
    [Google Scholar]
  4. Chen L.-L., Wang H.-C., Huang C.-J. 9 other authors 2002b; Transcriptional analysis of the DNA polymerase gene of shrimp white spot syndrome virus. Virology 301:136–147 [CrossRef]
    [Google Scholar]
  5. Corbel V., Zuprizal, Shi Z., Huang C., Arcier J.-M., Bonami J.-R. 2001; Experimental infection of European crustaceans with white spot syndrome virus (WSSV). J Fish Dis 24:377–382 [CrossRef]
    [Google Scholar]
  6. Huang C., Zhang X., Lin Q., Xu X., Hew C.-L. 2002a; Characterization of a novel envelope protein (VP281) of shrimp white spot syndrome virus by mass spectrometry. J Gen Virol 83:2385–2392
    [Google Scholar]
  7. Huang C., Zhang X., Lin Q., Xu X., Hu Z., Hew C.-L. 2002b; Proteomic analysis of shrimp white spot syndrome viral proteins and characterization of a novel envelope protein VP466. Mol Cell Proteomics 1:223–231 [CrossRef]
    [Google Scholar]
  8. Kalvakolanu D. V. 1999; Virus interception of cytokine-regulated pathways. Trends Microbiol 7:166–171 [CrossRef]
    [Google Scholar]
  9. Laemmli U. K. 1970; Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature 227:680–685 [CrossRef]
    [Google Scholar]
  10. Leu J.-H., Tsai J.-M., Wang H.-C., Wang A. H.-J., Wang C.-H., Kou G.-H., Lo C.-F. 2005; The unique stacked rings in the nucleocapsid of the white spot syndrome virus virion are formed by the major structural protein VP664, the largest viral structural protein ever found. J Virol 79:140–149 [CrossRef]
    [Google Scholar]
  11. Li Q., Chen Y., Yang F. 2004; Identification of a collagen-like protein gene from white spot syndrome virus. Arch Virol 149:215–223 [CrossRef]
    [Google Scholar]
  12. Lightner D. V. 1996 A Handbook of Shrimp Pathology and Diagnostic Procedures for Diseases of Cultured Penaeid Shrimp Baton Rouge, LA: World Aquaculture Society;
    [Google Scholar]
  13. Lin S.-T., Chang Y.-S., Wang H.-C., Tzeng H.-F., Chang Z.-F., Lin J.-Y., Wang C.-H., Lo C.-F., Kou G.-H. 2002; Ribonucleotide reductase of shrimp white spot syndrome virus (WSSV): expression and enzymatic activity in a baculovirus/insect cell system and WSSV-infected shrimp. Virology 304:282–290 [CrossRef]
    [Google Scholar]
  14. Lo C.-F., Ho H.-C., Peng S.-E. 8 other authors 1996; White spot syndrome baculovirus (WSBV) detected in cultured and captured shrimp, crabs and other arthropods. Dis Aquat Organ 27:215–225 [CrossRef]
    [Google Scholar]
  15. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual , 2nd edn. Cold Spring Habor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  16. Takahashi Y., Itami T., Kondo M., Maeda M., Fujii R., Tomonaga S., Supamattaya K., Boonyaratpalin S. 1994; Electron microscopy evidence of bacilliform virus infection in Kuruma shrimp ( Penaeus japonicus ). Fish Pathol 29:121–125 [CrossRef]
    [Google Scholar]
  17. Tsai M.-F., Yu H.-T., Tzeng H.-F. 7 other authors 2000; Identification and characterization of a shrimp white spot syndrome virus (WSSV) gene that encodes a novel chimeric polypeptide of cellular-type thymidine kinase and thymidylate kinase. Virology 277:100–110 [CrossRef]
    [Google Scholar]
  18. Tsai J.-M., Wang H.-C., Leu J.-H., Hsiao H.-H., Wang A. H.-J., Kou G.-H., Lo C.-F. 2004; Genomic and proteomic analysis of thirty-nine structural proteins of shrimp white spot syndrome virus. J Virol 78:11360–11370 [CrossRef]
    [Google Scholar]
  19. Tzeng H.-F., Chang Z.-F., Peng S.-E., Wang C.-H., Lin J.-Y., Kou G.-H., Lo C.-F. 2002; Chimeric polypeptide of thymidine kinase and thymidylate kinase of shrimp white spot syndrome virus: thymidine kinase activity of the recombinant protein expressed in a baculovirus/insect cell system. Virology 299:248–255 [CrossRef]
    [Google Scholar]
  20. van Hulten M. C. W., Goldbach R. W., Vlak J. M. 2000a; Three functionally diverged major structural proteins of white spot syndrome virus evolved by gene duplication. J Gen Virol 81:2525–2529
    [Google Scholar]
  21. van Hulten M. C. W., Tsai M.-F., Schipper C. A., Lo C.-F., Kou G.-H., Vlak J. M. 2000b; Analysis of a genomic segment of white spot syndrome virus of shrimp containing ribonucleotide reductase genes and repeat regions. J Gen Virol 81:307–316
    [Google Scholar]
  22. van Hulten M. C. W., Westenberg M., Goodall S. D., Vlak J. M. 2000c; Identification of two major virion protein genes of white spot syndrome virus of shrimp. Virology 266:227–236 [CrossRef]
    [Google Scholar]
  23. van Hulten M. C. W., Witteveldt J., Peters S., Kloosterboer N., Tarchini R., Fiers M., Sandbrink H., Lankhorst R. K., Vlak J. M. 2001a; The white spot syndrome virus DNA genome sequence. Virology 286:7–22 [CrossRef]
    [Google Scholar]
  24. van Hulten M. C. W., Witteveldt J., Snippe M., Vlak J. M. 2001b; White spot syndrome virus envelope protein VP28 is involved in the systemic infection of shrimp. Virology 285:228–233 [CrossRef]
    [Google Scholar]
  25. van Hulten M. C. W., Reijns M., Vermeesch A. M. G., Zandbergen F., Vlak J. M. 2002; Identification of VP19 and VP15 of white spot syndrome virus (WSSV) and glycosylation status of the WSSV major structural proteins. J Gen Virol 83:257–265
    [Google Scholar]
  26. Wang C.-H., Lo C.-F., Leu J.-H. 7 other authors 1995; Purification and genomic analysis of baculovirus associated with white spot syndrome (WSBV) of Penaeus monodon . Dis Aquat Organ 23:239–242 [CrossRef]
    [Google Scholar]
  27. Witteveldt J., van Hulten M. C. W., Vlak J. M. 2001; Identification and phylogeny of a non-specific endonuclease gene of white spot syndrome virus of shrimp. Virus Genes 23:331–337 [CrossRef]
    [Google Scholar]
  28. Yang F., He J., Lin X., Li Q., Pan D., Zhang X., Xu X. 2001; Complete genome sequence of the shrimp white spot bacilliform virus. J Virol 75:11811–11820 [CrossRef]
    [Google Scholar]
  29. Zhang X., Xu X., Hew C.-L. 2001; The structure and function of a gene encoding a basic peptide from prawn white spot syndrome virus. Virus Res 79:137–144 [CrossRef]
    [Google Scholar]
  30. Zhang X., Huang C., Xu X., Hew C.-L. 2002a; Identification and localization of a prawn white spot syndrome virus gene that encodes an envelope protein. J Gen Virol 83:1069–1074
    [Google Scholar]
  31. Zhang X., Huang C., Xu X., Hew C.-L. 2002b; Transcription and identification of an envelope protein gene (p22) from shrimp white spot syndrome virus. J Gen Virol 83:471–477
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.80923-0
Loading
/content/journal/jgv/10.1099/vir.0.80923-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed