1887

Abstract

To evaluate the usefulness of detection of antibodies to hepatitis E virus (HEV) to screen for viraemic pigs, serum samples obtained from 1425 1–6-month-old pigs in Japan were tested for swine HEV RNA and IgG, IgM and IgA classes of anti-HEV antibody. Fifty-five (5 %) of the 1071 2–5-month-old pigs were positive for swine HEV RNA, but none of 218 1-month-old pigs or 136 6-month-old pigs had detectable HEV RNA. The prevalence of anti-HEV IgG among the viraemic pigs (67 %, 37/55) was similar to that among the non-viraemic pigs (55 %, 757/1370) and the prevalence of anti-HEV IgM among the viraemic pigs and non-viraemic pigs was 7 and 3 %, respectively. However, anti-HEV IgA was detected significantly more frequently among viraemic pigs than among non-viraemic pigs (55 vs 10 %, <0·0001). These results suggest that anti-HEV IgA is more useful than anti-HEV IgM to screen for viraemic pigs.

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2005-06-01
2019-10-16
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References

  1. Arankalle, V. A., Chobe, L. P., Joshi, M. V., Chadha, M. S., Kundu, B. & Walimbe, A. M. ( 2002; ). Human and swine hepatitis E viruses from Western India belong to different genotypes. J Hepatol 36, 417–425.[CrossRef]
    [Google Scholar]
  2. Chandler, J. D., Riddell, M. A., Li, F., Love, R. J. & Anderson, D. A. ( 1999; ). Serological evidence for swine hepatitis E virus infection in Australian pig herds. Vet Microbiol 68, 95–105.[CrossRef]
    [Google Scholar]
  3. Chau, K. H., Dawson, G. J., Bile, K. M., Magnius, L. O., Sjogren, M. H. & Mushahwar, I. K. ( 1993; ). Detection of IgA class antibody to hepatitis E virus in serum samples from patients with hepatitis E virus infection. J Med Virol 40, 334–338.[CrossRef]
    [Google Scholar]
  4. Choi, I.-S., Kwon, H.-J., Shin, N.-R. & Yoo, H. S. ( 2003; ). Identification of swine hepatitis E virus (HEV) and prevalence of anti-HEV antibodies in swine and human populations in Korea. J Clin Microbiol 41, 3602–3608.[CrossRef]
    [Google Scholar]
  5. Clayson, E. T., Innis, B. L., Myint, K. S. A., Narupiti, S., Vaughn, D. W., Giri, S., Ranabhat, P. & Shrestha, M. P. ( 1995; ). Detection of hepatitis E virus infections among domestic swine in the Kathmandu Valley of Nepal. Am J Trop Med Hyg 53, 228–232.
    [Google Scholar]
  6. Erker, J. C., Desai, S. M., Schlauder, G. G., Dawson, G. J. & Mushahwar, I. K. ( 1999; ). A hepatitis E virus variant from the United States: molecular characterization and transmission in cynomolgus macaques. J Gen Virol 80, 681–690.
    [Google Scholar]
  7. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  8. Garkavenko, O., Obriadina, A., Meng, J., Anderson, D. A., Benard, H. J., Schroeder, B. A., Khudyakov, Y. E., Fields, H. A. & Croxson, M. C. ( 2001; ). Detection and characterization of swine hepatitis E virus in New Zealand. J Med Virol 65, 525–529.[CrossRef]
    [Google Scholar]
  9. Halbur, P. G., Kasorndorkbua, C., Gilbert, C., Guenette, D., Potters, M. B., Purcell, R. H., Emerson, S. U., Toth, T. E. & Meng, X. J. ( 2001; ). Comparative pathogenesis of infection of pigs with hepatitis E viruses recovered from a pig and a human. J Clin Microbiol 39, 918–923.[CrossRef]
    [Google Scholar]
  10. Harrison, T. J. ( 1999; ). Hepatitis E virus – an update. Liver 19, 171–176.
    [Google Scholar]
  11. Hsieh, S.-Y., Meng, X.-J., Wu, Y.-H., Liu, S.-T., Tam, A. W., Lin, D.-Y. & Liaw, Y.-F. ( 1999; ). Identity of a novel swine hepatitis E virus in Taiwan forming a monophyletic group with Taiwan isolates of human hepatitis E virus. J Clin Microbiol 37, 3828–3834.
    [Google Scholar]
  12. Huang, C.-C., Nguyen, D., Fernandez, J., Yun, K. Y., Fry, K. E., Bradley, D. W., Tam, A. W. & Reyes, G. R. ( 1992; ). Molecular cloning and sequencing of the Mexico isolate of hepatitis E virus (HEV). Virology 191, 550–558.[CrossRef]
    [Google Scholar]
  13. Huang, F. F., Haqshenas, G., Guenette, D. K., Halbur, P. G., Schommer, S. K., Pierson, F. W., Toth, T. E. & Meng, X. J. ( 2002a; ). Detection by reverse transcription-PCR and genetic characterization of field isolates of swine hepatitis E virus from pigs in different geographic regions of the United States. J Clin Microbiol 40, 1326–1332.[CrossRef]
    [Google Scholar]
  14. Huang, F. F., Haqshenas, G., Shivaprasad, H. L. & 7 other authors ( 2002b; ). Heterogeneity and seroprevalence of a newly identified avian hepatitis E virus from chickens in the United States. J Clin Microbiol 40, 4197–4202.[CrossRef]
    [Google Scholar]
  15. Huang, F. F., Sun, Z. F., Emerson, S. U., Purcell, R. H., Shivaprasad, H. L., Pierson, F. W., Toth, T. E. & Meng, X. J. ( 2004; ). Determination and analysis of the complete genomic sequence of avian hepatitis E virus (avian HEV) and attempts to infect rhesus monkeys with avian HEV. J Gen Virol 85, 1609–1618.[CrossRef]
    [Google Scholar]
  16. Ina, Y. ( 1994; ). oden: a program package for molecular evolutionary analysis and database search of DNA and amino acid sequences. Comput Appl Biosci 10, 11–12.
    [Google Scholar]
  17. Meng, X.-J. ( 2000; ). Novel strains of hepatitis E virus identified from humans and other animal species: is hepatitis E a zoonosis? J Hepatol 33, 842–845.[CrossRef]
    [Google Scholar]
  18. Meng, X.-J. ( 2003; ). Swine hepatitis E virus: cross-species infection and risk in xenotransplantation. Curr Top Microbiol Immunol 278, 185–216.
    [Google Scholar]
  19. Meng, X.-J., Purcell, R. H., Halbur, P. G., Lehman, J. R., Webb, D. M., Tsareva, T. S., Haynes, J. S., Thacker, B. J. & Emerson, S. U. ( 1997; ). A novel virus in swine is closely related to the human hepatitis E virus. Proc Natl Acad Sci U S A 94, 9860–9865.[CrossRef]
    [Google Scholar]
  20. Meng, X.-J., Halbur, P. G., Haynes, J. S., Tsareva, T. S., Bruna, J. D., Royer, R. L., Purcell, R. H. & Emerson, S. U. ( 1998a; ). Experimental infection of pigs with the newly identified swine hepatitis E virus (swine HEV), but not with human strains of HEV. Arch Virol 143, 1405–1415.[CrossRef]
    [Google Scholar]
  21. Meng, X.-J., Halbur, P. G., Shapiro, M. S., Govindarajan, S., Bruna, J. D., Mushahwar, I. K., Purcell, R. H. & Emerson, S. U. ( 1998b; ). Genetic and experimental evidence for cross-species infection by swine hepatitis E virus. J Virol 72, 9714–9721.
    [Google Scholar]
  22. Meng, X.-J., Dea, S., Engle, R. E. & 10 other authors ( 1999; ). Prevalence of antibodies to the hepatitis E virus in pigs from countries where hepatitis E is common or is rare in the human population. J Med Virol 59, 297–302.[CrossRef]
    [Google Scholar]
  23. Mitsui, T., Tsukamoto, Y., Yamazaki, C., Masuko, K., Tsuda, F., Takahashi, M., Nishizawa, T. & Okamoto, H. ( 2004; ). Prevalence of hepatitis E virus infection among hemodialysis patients in Japan: evidence for infection with a genotype 3 HEV by blood transfusion. J Med Virol 74, 563–572.[CrossRef]
    [Google Scholar]
  24. Mizuo, H., Suzuki, K., Takikawa, Y. & 8 other authors ( 2002; ). Polyphyletic strains of hepatitis E virus are responsible for sporadic cases of acute hepatitis in Japan. J Clin Microbiol 40, 3209–3218.[CrossRef]
    [Google Scholar]
  25. Nishizawa, T., Takahashi, M., Mizuo, H., Miyajima, H., Gotanda, Y. & Okamoto, H. ( 2003; ). Characterization of Japanese swine and human hepatitis E virus isolates of genotype IV with 99 % identity over the entire genome. J Gen Virol 84, 1245–1251.[CrossRef]
    [Google Scholar]
  26. Okamoto, H., Takahashi, M., Nishizawa, T., Fukai, K., Muramatsu, U. & Yoshikawa, A. ( 2001; ). Analysis of the complete genome of indigenous swine hepatitis E virus isolated in Japan. Biochem Biophys Res Commun 289, 929–936.[CrossRef]
    [Google Scholar]
  27. Okamoto, H., Takahashi, M. & Nishizawa, T. ( 2003; ). Features of hepatitis E virus infection in Japan. Intern Med 42, 1065–1071.[CrossRef]
    [Google Scholar]
  28. Pei, Y. & Yoo, D. ( 2002; ). Genetic characterization and sequence heterogeneity of a Canadian isolate of swine hepatitis E virus. J Clin Microbiol 40, 4021–4029.[CrossRef]
    [Google Scholar]
  29. Pina, S., Buti, M., Cotrina, M., Piella, J. & Girones, R. ( 2000; ). HEV identified in serum from humans with acute hepatitis and in sewage of animal origin in Spain. J Hepatol 33, 826–833.[CrossRef]
    [Google Scholar]
  30. Purcell, R. H. & Emerson, S. U. ( 2001; ). Hepatitis E virus. In Fields Virology, 4th edn, pp. 3051–3061. Edited by D. M. Knipe, P. M. Howley, D. E. Griffin, R. A. Lamb, M. A. Martin, B. Roizman & S. E. Straus. Philadelphia, PA: Lippincott Williams & Wilkins.
  31. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  32. Schlauder, G. G. & Mushahwar, I. K. ( 2001; ). Genetic heterogeneity of hepatitis E virus. J Med Virol 65, 282–292.[CrossRef]
    [Google Scholar]
  33. Smith, J. L. ( 2001; ). A review of hepatitis E virus. J Food Prot 64, 572–586.
    [Google Scholar]
  34. Takahashi, M., Nishizawa, T., Miyajima, H., Gotanda, Y., Iita, T., Tsuda, F. & Okamoto, H. ( 2003a; ). Swine hepatitis E virus strains in Japan form four phylogenetic clusters comparable with those of Japanese isolates of human hepatitis E virus. J Gen Virol 84, 851–862.[CrossRef]
    [Google Scholar]
  35. Takahashi, M., Nishizawa, T. & Okamoto, H. ( 2003b; ). Identification of a genotype III swine hepatitis E virus that was isolated from a Japanese pig born in 1990 and that is most closely related to Japanese isolates of human hepatitis E virus. J Clin Microbiol 41, 1342–1343.[CrossRef]
    [Google Scholar]
  36. Takahashi, M., Kusakai, S., Mizuo, H. & 7 other authors ( 2005; ). Simultaneous detection of immunoglobulin A (IgA) and IgM antibodies against hepatitis E virus (HEV) is highly specific for diagnosis of acute HEV infection. J Clin Microbiol 43, 49–56.[CrossRef]
    [Google Scholar]
  37. Tam, A. W., Smith, M. M., Guerra, M. E., Huang, C.-C., Bradley, D. W., Fry, K. E. & Reyes, G. R. ( 1991; ). Hepatitis E virus (HEV): molecular cloning and sequencing of the full-length viral genome. Virology 185, 120–131.[CrossRef]
    [Google Scholar]
  38. Tanaka, H., Yoshino, H., Kobayashi, E., Takahashi, M. & Okamoto, H. ( 2004; ). Molecular investigation of hepatitis E virus infection in domestic and miniature pigs used for medical experiments. Xenotransplantation 11, 503–510.[CrossRef]
    [Google Scholar]
  39. Tei, S., Kitajima, N., Takahashi, K. & Mishiro, S. ( 2003; ). Zoonotic transmission of hepatitis E virus from deer to human beings. Lancet 362, 371–373.[CrossRef]
    [Google Scholar]
  40. Tokita, H., Harada, H., Gotanda, Y., Takahashi, M., Nishizawa, T. & Okamoto, H. ( 2003; ). Molecular and serological characterization of sporadic acute hepatitis E in a Japanese patient infected with a genotype III hepatitis E virus in 1993. J Gen Virol 84, 421–427.[CrossRef]
    [Google Scholar]
  41. Usui, R., Kobayashi, E., Takahashi, M., Nishizawa, T. & Okamoto, H. ( 2004; ). Presence of antibodies to hepatitis E virus in Japanese pet cats. Infection 32, 57–58.[CrossRef]
    [Google Scholar]
  42. van der Poel, W. H., Verschoor, F., van der Heide, R., Herrera, M.-I., Vivo, A., Kooreman, M. & de Roda Husman, A. M. ( 2001; ). Hepatitis E virus sequences in swine related to sequences in humans, the Netherlands. Emerg Infect Dis 7, 970–976.[CrossRef]
    [Google Scholar]
  43. Wang, Y., Zhang, H., Ling, R., Li, H. & Harrison, T. J. ( 2000; ). The complete sequence of hepatitis E virus genotype 4 reveals an alternative strategy for translation of open reading frames 2 and 3. J Gen Virol 81, 1675–1686.
    [Google Scholar]
  44. Wang, Y.-C., Zhang, H., Xia, N. & 11 other authors ( 2002; ). Prevalence, isolation, and partial sequence analysis of hepatitis E virus from domestic animals in China. J Med Virol 67, 516–521.[CrossRef]
    [Google Scholar]
  45. Wibawa, I. D. N., Muljono, D. H., Mulyanto, Suryadarma, I. G. A., Tsuda, F., Takahashi, M., Nishizawa, T. & Okamoto, H. ( 2004; ). Prevalence of antibodies to hepatitis E virus among apparently healthy humans and pigs in Bali, Indonesia: identification of a pig infected with a genotype 4 hepatitis E virus. J Med Virol 73, 38–44.[CrossRef]
    [Google Scholar]
  46. Wu, J.-C., Chen, C.-M., Chiang, T.-Y., Tsai, W.-H., Jeng, W.-J., Sheen, I.-J., Kin, C.-C. & Meng, X.-J. ( 2002; ). Spread of hepatitis E virus among different-aged pigs: two-year survey in Taiwan. J Med Virol 66, 488–492.[CrossRef]
    [Google Scholar]
  47. Yazaki, Y., Mizuo, H., Takahashi, M., Nishizawa, T., Sasaki, N., Gotanda, Y. & Okamoto, H. ( 2003; ). Sporadic acute or fulminant hepatitis E in Hokkaido, Japan, may be food-borne, as suggested by the presence of hepatitis E virus in pig liver as food. J Gen Virol 84, 2351–2357.[CrossRef]
    [Google Scholar]
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vol. , part 6, pp. 1807 – 1813

Comparison of swine HEV isolates of genotypes 3 and 4 in Japan with 467 reported HEV isolates of genotype 1, 2, 3 or 4 whose common 239–412 nt sequence is known

Age-dependent prevalence of anti-HEV IgG in the sera of farm pigs in 20 prefectures in Japan

Neighbour-joining trees based on the partial nucleotide sequence of the ORF2 region of 121 genotype 3 and 64 genotype 4 HEV isolates

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