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Abstract

Partial sequences of a novel papillomavirus were amplified from a cutaneous lesion biopsy of a raccoon (), by using PCR with degenerate papillomavirus-specific primers. The papillomavirus type 1 (PlPV-1) DNA was amplified with long template PCR in two overlapping fragments, together encompassing the entire genome, and the complete PlPV-1 genomic sequence was determined. The PlPV-1 genome consists of 8170 bp, and contains the typical papillomaviral open reading frames, encoding five early proteins and two late capsid proteins. Besides the classical non-coding region (NCR1) between the end of L1 and the start of E6, PlPV-1 contains an additional non-coding region (NCR2) of 1065 bp between the early and late protein region, which has previously also been described for the canine oral papillomavirus (COPV) and the papillomavirus (FdPV-1). Phylogenetic analysis places PlPV-1 together with COPV and FdPV-1 in a monophyletic branch which encompasses the Lambda papillomavirus genus.

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2005-07-01
2020-01-20
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References

  1. Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J. ( 1990; ). Basic local alignment search tool. J Mol Biol 215, 403–410.[CrossRef]
    [Google Scholar]
  2. Bininda-Emonds, O. R., Gittleman, J. L. & Purvis, A. ( 1999; ). Building large trees by combining phylogenetic information: a complete phylogeny of the extant Carnivora (Mammalia). Biol Rev Camb Philos Soc 74, 143–175.[CrossRef]
    [Google Scholar]
  3. Delius, H., Van Ranst, M. A., Jenson, A. B., zur Hausen, H. & Sundberg, J. P. ( 1994; ). Canine oral papillomavirus genomic sequence: a unique 1·5-kb intervening sequence between the E2 and L2 open reading frames. Virology 204, 447–452.[CrossRef]
    [Google Scholar]
  4. de Villiers, E. M., Fauquet, C., Broker, T. R., Bernard, H. U. & zur Hausen, H. ( 2004; ). Classification of papillomaviruses. Virology 324, 17–27.[CrossRef]
    [Google Scholar]
  5. Flynn, J. J. & Nedbal, M. A. ( 1998; ). Phylogeny of the Carnivora (Mammalia): congruence vs incompatibility among multiple data sets. Mol Phylogenet Evol 9, 414–426.[CrossRef]
    [Google Scholar]
  6. Groff, D. E. & Lancaster, W. D. ( 1985; ). Molecular cloning and nucleotide sequence of deer papillomavirus. J Virol 56, 85–91.
    [Google Scholar]
  7. Hamir, A. N., Moser, G., Jenson, A. B., Sundberg, J. P., Hanlon, C. & Rupprecht, C. E. ( 1995; ). Papillomavirus infection in raccoons (Procyon lotor). J Vet Diagn Invest 7, 549–551.[CrossRef]
    [Google Scholar]
  8. Kumar, S., Tamura, K., Jakobsen, I. B. & Nei, M. ( 2001; ). mega2: molecular evolutionary genetics analysis software. Bioinformatics 17, 1244–1245.[CrossRef]
    [Google Scholar]
  9. Paterson, A. M. & Banks, J. ( 2001; ). Analytical approaches to measuring cospeciation of host and parasites: through a glass, darkly. Int J Parasitol 31, 1012–1022.[CrossRef]
    [Google Scholar]
  10. Rector, A., Bossart, G. D., Ghim, S. J., Sundberg, J. P., Jenson, A. B. & Van Ranst, M. ( 2004; ). Characterization of a novel close-to-root papillomavirus from a Florida manatee by using multiply primed rolling-circle amplification: Trichechus manatus latirostris papillomavirus type 1. J Virol 78, 12698–12702.[CrossRef]
    [Google Scholar]
  11. Rector, A., Tachezy, R., Van Doorslaer, K., MacNamara, T., Burk, R. D., Sundberg, J. P. & Van Ranst, M. ( 2005; ). Isolation and cloning of a papillomavirus from a North American porcupine by using multiply primed rolling-circle amplification: the Erethizon dorsatum papillomavirus type 1. Virology 331, 449–456.[CrossRef]
    [Google Scholar]
  12. Sundberg, J. P. ( 1987; ). Papillomavirus infections in animals. In Papillomaviruses and Human Disease. Edited by K. Syrjänen, L. Gissmann & L. G. Koss. Berlin: Springer.
  13. Sundberg, J. P., Van Ranst, M., Burk, R. D. & Jenson, A. B. ( 1997; ). The nonhuman (animal) papillomaviruses: host range, epitope conservation, and molecular diversity. In Human Papillomavirus Infections in Dermatovenereology. Edited by G. Gross & G. von Krogh. Boca Raton: CRC Press.
  14. Sundberg, J. P., Van Ranst, M., Montali, R. & 8 other authors ( 2000; ). Feline papillomas and papillomaviruses. Vet Pathol 37, 1–10.[CrossRef]
    [Google Scholar]
  15. Sundberg, J. P., Van Ranst, M. & Jenson, A. B. ( 2001; ). Papillomavirus infections. In Infectious Diseases of Wild Mammals. Edited by E. S. Williams & I. K. Barker. Ames: Iowa State University Press.
  16. Tachezy, R., Duson, G., Rector, A., Jenson, A. B., Sundberg, J. P. & Van Ranst, M. ( 2002a; ). Cloning and genomic characterization of Felis domesticus papillomavirus type 1. Virology 301, 313–321.[CrossRef]
    [Google Scholar]
  17. Tachezy, R., Rector, A., Havelkova, M., Wollants, E., Fiten, P., Opdenakker, G., Jenson, B., Sundberg, J. & Van Ranst, M. ( 2002b; ). Avian papillomaviruses: the parrot Psittacus erithacus papillomavirus (PePV) genome has a unique organization of the early protein region and is phylogenetically related to the chaffinch papillomavirus. BMC Microbiol 2, 19.[CrossRef]
    [Google Scholar]
  18. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  19. Van Ranst, M., Kaplan, J. B. & Burk, R. D. ( 1992; ). Phylogenetic classification of human papillomaviruses: correlation with clinical manifestations. J Gen Virol 73, 2653–2660.[CrossRef]
    [Google Scholar]
  20. Van Ranst, M., Kaplan, J. B., Sundberg, J. P. & Burk, R. D. ( 1995; ). Molecular evolution of papillomaviruses. In Molecular Basis of Virus Evolution. Edited by A. Gibbs, C. H. Calisher & F. Garcia-Arenal. Cambridge: Cambridge University Press.
  21. Xia, X. & Xie, Z. ( 2001; ). dambe: software package for data analysis in molecular biology and evolution. J Hered 92, 371–373.[CrossRef]
    [Google Scholar]
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vol. , part 7, pp. 2029 - 2033

Amplification of partial E1 and L1 sequences of PlPV-1 with degenerate PV-specific primers. [PDF](141 KB)



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