1887

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Volume 86, Issue 8

The hydrophobic segment of TGBp3 is a major determinant of the protein intracellular trafficking Free

Abstract

(PVX) encodes three movement proteins, TGBp1, TGBp2 and TGBp3. The 8 kDa TGBp3 is a membrane-embedded protein that has an N-terminal hydrophobic sequence segment and a hydrophilic C terminus. TGBp3 mutants with deletions in the C-terminal hydrophilic region retain the ability to be targeted to cell peripheral structures and to support limited PVX cell-to-cell movement, suggesting that the basic TGBp3 functions are associated with its N-terminal transmembrane region. Fusion of green fluorescent protein to the TGBp3 N terminus abrogates protein activities in intracellular trafficking and virus movement. The intracellular transport of TGBp3 from sites of its synthesis in the rough endoplasmic reticulum (ER) to ER-derived peripheral bodies involves a non-conventional COPII-independent pathway. However, integrity of the C-terminal hydrophilic sequence is required for entrance to this non-canonical route.

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2005-08-01
2024-04-19
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The hydrophobic segment of Potato virus X TGBp3 is a major determinant of the protein intracellular trafficking
J. Gen. Virol. 86, 2379 (2005); https://doi.org/10.1099/vir.0.80865-0
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