1887

Abstract

larvae are highly resistant to oral infection by (AcMNPV) (LD, ∼9200 occlusions), but extremely susceptible to budded virus within the haemocoel (LD, <1 p.f.u.). The inability of AcMNPV occlusion-derived virus (ODV) to establish primary infections readily within midgut cells accounts for a major proportion of oral resistance. To determine whether inappropriate binding of AcMNPV ODV to midgut cells contributes to lack of oral infectivity, the binding and fusion properties of AcMNPV ODV were compared with those of the ODV of a new isolate of (SfMNPV) obtained from a field-collected larva (oral LD, 12 occlusions). By using a fluorescence-dequenching assay conducted , it was found that AcMNPV ODV bound to the midgut epithelia of larvae at ∼15 % of the level of SfMNPV ODV, but that, once bound, the efficiencies of fusion for the two ODVs were similar: 60 % for AcMNPV and 53 % for SfMNPV. Whilst the difference in binding efficiencies was significant, it could not account entirely for the observed differences in infectivity. Competition experiments, however, revealed that, in larvae, SfMNPV ODV bound to a midgut cell receptor that was not bound by AcMNPV ODV, indicating that ODV interaction with a specific receptor(s) was necessary for productive infection of midgut columnar epithelial cells. Fusion in the absence of this ligand–receptor interaction did not result in productive infections.

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2005-05-01
2019-10-21
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