1887

Journal of General Virology

Volume 86, Issue 5

Rolling-circle amplification of (TTV) complete genomes from human and swine sera and identification of a novel swine TTV genogroup Free

Abstract

Multiply primed rolling-circle amplification is a novel technology that uses bacteriophage phi29 DNA polymerase to amplify circular DNA molecules, without the need for prior knowledge of their sequences. In an attempt to detect (TTV), rolling-circle amplification was used to amplify DNA extracted from eight human and four pig serum samples. All samples gave high molecular weight (>30 kb) amplification products. By restriction endonuclease digestion, these products generated DNA fragments whose sizes were consistent with those of human TTV (3·8 kb) and swine TTV (Sd-TTV; 2·9 kb) genomes. Two TTV isolates derived from a single AIDS patient, as well as two Sd-TTV isolates derived from a single pig, were characterized by complete nucleotide sequencing. One of the Sd-TTV isolates showed very low (43–45 %) nucleotide sequence similarity to the other Sd-TTV isolate and to the prototype isolate Sd-TTV31, and could be considered the prototype of a novel genogroup.

  • Received:
  • Accepted:
  • Published Online:
© SGM
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2005-05-01
2019-10-17
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Rolling-circle amplification of Torque teno virus (TTV) complete genomes from human and swine sera and identification of a novel swine TTV genogroup
J. Gen. Virol. 86, 1343 (2005); https://doi.org/10.1099/vir.0.80794-0
/content/journal/jgv/10.1099/vir.0.80794-0
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