RT Journal Article SR Electronic(1) A1 Meschi, Joseph A1 Crouch, Erika C. A1 Skolnik, Paul A1 Yahya, Khabirah A1 Holmskov, Uffe A1 Leth-Larsen, Rikke A1 Tornoe, Ida A1 Tecle, Tesfaldet A1 White, Mitchell R. A1 Hartshorn, Kevan L.YR 2005 T1 Surfactant protein D binds to human immunodeficiency virus (HIV) envelope protein gp120 and inhibits HIV replication JF Journal of General Virology, VO 86 IS 11 SP 3097 OP 3107 DO https://doi.org/10.1099/vir.0.80764-0 PB Microbiology Society, SN 1465-2099, AB The envelope protein (gp120) of human immunodeficiency virus (HIV) contains highly conserved mannosylated oligosaccharides. These glycoconjugates contribute to resistance to antibody neutralization, and binding to cell surface lectins on macrophages and dendritic cells. Mannose-binding lectin (MBL) binds to gp120 and plays a role in defence against the virus. In this study it is demonstrated that surfactant protein D (SP-D) binds to gp120 and inhibits HIV infectivity at significantly lower concentrations than MBL. The binding of SP-D was mediated by its calcium-dependent carbohydrate-binding activity and was dependent on glycosylation of gp120. Native dodecameric SP-D bound to HIV gp120 more strongly than native trimeric SP-D. Since one common polymorphic form of SP-D is predominantly expressed as trimers and associated with lower blood levels, these individuals may have less effective innate defence against HIV. A chimeric protein containing the N-terminal and collagen domains of SP-D linked to the neck and carbohydrate-recognition domains of MBL (called SP-D/MBLneck+CRD) had greater ability to bind to gp120 and inhibit virus replication than either SP-D or MBL. The enhanced binding of SP-D/MBLneck+CRD was dependent on assembly into higher molecular mass multimers (i.e. a trimeric form of the chimera did not bind to a greater extent than MBL). Hence, the enhanced binding of SP-D compared with MBL results from distinctive properties of its N-terminal and/or collagen domains. SP-D is present in lung and airway fluids, as well as in blood and various mucosal locations, and could, like MBL, play a role in restricting HIV transmission or replication in vivo., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.80764-0