1887

Abstract

Prolonged infection of tomato hybrid (×) by (CEVd) resulted in viroid-like enlarged structures, detected by gel electrophoresis. This population included two new enlarged variants or D-variants, D-87 and D-76, and three transient species or D-forms, D-38, D-40 and D-43. Sequence analyses exposed a locus near the terminal repeat region where major changes appeared consistently. In transmission tests to CEVd hosts, a variety of progeny populations were recovered, including progeny enlargements of and reversions to CEVd, as well as sequence fidelity to the inoculum. Transmission tests to citrus hosts of the genera , or were unsuccessful. The importance of host specificity to the recovery and processing of the various CEVd-related structures, as well as the temporal variability of progeny populations, was demonstrated.

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2005-02-01
2021-03-08
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References

  1. Fadda Z., Daròs J. A., Flores R., Duran-Vila N. 2003; Identification in eggplant of a variant of citrus exocortis viroid (CEVd) with a 96 nucleotide duplication in the right terminal region of the rod-like secondary structure. Virus Res 97:145–149 [CrossRef]
    [Google Scholar]
  2. Fawcett H. S., Klotz L. J. 1948; Exocortis on trifoliate orange. Citrus Leaves 28:8
    [Google Scholar]
  3. Flores R., Di Serio F., Hernández C. 1997; Viroids: the noncoding genomes. Semin Virol 8:65–73 [CrossRef]
    [Google Scholar]
  4. Gozmanova M., Denti M. A., Minkov I. N., Tsagris M., Tabler M. 2003; Characterization of the RNA motif responsible for the specific interaction of potato spindle tuber viroid RNA (PSTVd) and the tomato protein Virp1. Nucleic Acids Res 31:5534–5543 [CrossRef]
    [Google Scholar]
  5. Gross H. J., Krupp G., Domdey H., Raba M., Jank P., Lossow C., Alberty H., Ramm K., Sänger H. L. 1982; Nucleotide sequence and secondary structure of citrus exocortis and chrysanthemum stunt viroid. Eur J Biochem 121:249–257 [CrossRef]
    [Google Scholar]
  6. Haseloff J., Mohamed N. A., Symons R. H. 1982; Viroid RNAs of cadang-cadang disease of coconuts. Nature 299:316–321 [CrossRef]
    [Google Scholar]
  7. Igloi G. L. 1983; A silver stain for the detection of nanogram amounts of tRNA following two-dimensional electrophoresis. Anal Biochem 134:184–188 [CrossRef]
    [Google Scholar]
  8. Keese P., Symons R. H. 1985; Domains in viroids: evidence of intermolecular RNA rearrangements and their contribution to viroid evolution. Proc Natl Acad Sci U S A 82:4582–4586 [CrossRef]
    [Google Scholar]
  9. Loss P., Schmitz M., Steger G., Riesner D. 1991; Formation of a thermodynamically metastable structure containing hairpin II is critical for infectivity of potato spindle tuber viroid RNA. EMBO J 10:719–727
    [Google Scholar]
  10. Owens R. A., Hammond R. W., Gardner R. C., Kiefer M. C., Thompson S. M., Cress D. E. 1986; Site-specific mutagenesis of potato spindle tuber viroid cDNA: alterations within premelting region 2 that abolish infectivity. Plant Mol Biol 6:179–192 [CrossRef]
    [Google Scholar]
  11. Rivera-Bustamante R., Gin R., Semancik J. S. 1986; Enhanced resolution of circular and linear molecular forms of viroid and viroid-like RNA by electrophoresis in a discontinuous-pH system. Anal Biochem 156:91–95 [CrossRef]
    [Google Scholar]
  12. Semancik J. S., Duran-Vila N. 1999; Viroids in plants: shadows and footprints of a primitive RNA. In Origin and Evolution of Viruses pp  37–64 Edited by Domingo E., Webster R., Holland J. San Diego: Academic Press;
    [Google Scholar]
  13. Semancik J. S., Roistacher C. N., Rivera-Bustamante R., Duran-Vila N. 1988; Citrus cachexia viroid, a new viroid of citrus: relationship to viroids of the exocortis disease complex. J Gen Virol 69:3059–3068 [CrossRef]
    [Google Scholar]
  14. Semancik J. S., Szychowski J. A., Rakowski A. G., Symons R. H. 1993; Isolates of citrus exocortis viroid recovered by host and tissue selection. J Gen Virol 74:2427–2436 [CrossRef]
    [Google Scholar]
  15. Semancik J. S., Szychowski J. A., Rakowski A. G., Symons R. H. 1994; A stable 436 nucleotide variant of citrus exocortis viroid produced by terminal repeats. J Gen Virol 75:727–732 [CrossRef]
    [Google Scholar]
  16. Steger G., Riesner D. 2003; Molecular characteristics. In Viroids pp  15–29 Edited by Hadidi A., Flores R., Randles J. W., Semancik J. S. Collingwood, Australia: CSIRO;
    [Google Scholar]
  17. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  18. Visvader J. E., Forster A. C., Symons R. H. 1985; Infectivity and in vitro mutagenesis of monomeric cDNA clones of citrus exocortis viroid indicates the site of processing of viroid precursors. Nucleic Acids Res 13:5843–5856 [CrossRef]
    [Google Scholar]
  19. Wassenegger M., Heimes S., Sänger H. L. 1994; An infectious viroid RNA replicon evolved from an in vitro -generated non-infectious viroid deletion mutant via a complementary deletion in vivo . EMBO J 13:6172–6177
    [Google Scholar]
  20. Zuker M. 1989; On finding all suboptimal foldings of an RNA molecule. Science 244:48–52 [CrossRef]
    [Google Scholar]
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