1887

Abstract

Routine antibody surveillance for in a breeding colony suggested viral invasion into laboratory rats. A more specific haemagglutination-inhibition test implied that the agent was related closely to (hPIV3), rather than . To isolate this virus, Vero cells were inoculated with lung homogenates of 30 young animals from the colony. One of the cultures became positive at the second passage by RT-PCR directed to the hPIV3 NP and L genes. Cytopathic effect with cell fusion was observed at the third passage. The HN gene of this virus (KK24) had >93 % similarity to those of other hPIV3 isolates, suggesting a human origin of KK24. Experimental intranasal inoculation of KK24 into SD rats showed virus replication in the lungs at 3–5 days post-infection (p.i.). Pathological examination of the lungs at day 5 p.i. indicated a moderate detachment, degradation and apoptosis of bronchial epitheliocytes with peribronchial mononuclear infiltrations. At day 7 p.i., these changes became less prominent, and no lesions were apparent at day 10 p.i. or later. The infected rats seroconverted at day 7 p.i. On the contrary, none of the 30 experimentally infected ICR mice showed any pathological lesions in their lungs, despite seroconversion at 7 days p.i. These results suggest that hPIV3 can invade rat colonies and has a moderate and transient pathogenicity in rats. This is the first report of non-experimental hPIV3 infection in laboratory rats, unexpectedly detected by antibody screening for .

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.80666-0
2005-03-01
2019-11-12
Loading full text...

Full text loading...

/deliver/fulltext/jgv/86/3/vir860733.html?itemId=/content/journal/jgv/10.1099/vir.0.80666-0&mimeType=html&fmt=ahah

References

  1. Bellini, W. J., Rota, P. A. & Anderson, L. J. ( 1998; ). Paramyxoviruses. In Topley & Wilson's Microbiology and Microbial Infections, 9th edition, pp. 435–461. Edited by B. W. J. Mahy & L. Collier. London: Arnold.
  2. Castleman, W. L., Brundage-Anguish, L. J., Kreitzer, L. & Neuenschwander, S. B. ( 1987; ). Pathogenesis of bronchiolitis and pneumonia induced in neonatal and weanling rats by parainfluenza (Sendai) virus. Am J Pathol 129, 277–286.
    [Google Scholar]
  3. Craighead, J. E., Cook, M. K. & Chanock, R. M. ( 1960; ). Infection of hamsters with para influenza 3 virus. Proc Soc Exp Biol Med 104, 301–304.[CrossRef]
    [Google Scholar]
  4. Felsenstein, J. ( 1992; ). Estimating effective population size from samples of sequences: inefficiency of pairwise and segregating sites as compared to phylogenetic estimates. Genet Res 59, 139–147.[CrossRef]
    [Google Scholar]
  5. Henricks, P. A., Van Esch, B., Engels, F. & Nijkamp, F. P. ( 1993; ). Effects of parainfluenza type 3 virus on guinea pig pulmonary alveolar macrophage functions in vitro. Inflammation 17, 663–675.[CrossRef]
    [Google Scholar]
  6. Holmes, M. J. & Ramsay, A. J. ( 1988; ). Rabbit model for mucosal immunity in the bowel. I. Establishment of virus-infected ileal loops. J Med Virol 25, 271–280.[CrossRef]
    [Google Scholar]
  7. Ito, Y., Tsurudome, M., Hishiyama, M. & Yamada, A. ( 1987; ). Immunological interrelationships among human and non-human paramyxoviruses revealed by immunoprecipitation. J Gen Virol 68, 1289–1297.[CrossRef]
    [Google Scholar]
  8. Murphy, T. F., Dubovi, E. J. & Clyde, W. A., Jr ( 1981; ). The cotton rat as an experimental model of human parainfluenza virus type 3 disease. Exp Lung Res 2, 97–109.[CrossRef]
    [Google Scholar]
  9. Ohsawa, K., Yamada, A., Takeuchi, K., Watanabe, Y., Miyata, H. & Sato, H. ( 1998; ). Genetic characterization of parainfluenza virus 3 derived from guinea pigs. J Vet Med Sci 60, 919–922.[CrossRef]
    [Google Scholar]
  10. Ottolini, M. G., Porter, D. D., Blanco, J. C. G. & Prince, G. A. ( 2002; ). A cotton rat model of human parainfluenza 3 laryngotracheitis: virus growth, pathology, and therapy. J Infect Dis 186, 1713–1717.[CrossRef]
    [Google Scholar]
  11. Porter, D. D., Prince, G. A., Hemming, V. G. & Porter, H. G. ( 1991; ). Pathogenesis of human parainfluenza virus 3 infection in two species of cotton rats: Sigmodon hispidus develops bronchiolitis, while Sigmodon fulviventer develops interstitial pneumonia. J Virol 65, 103–111.
    [Google Scholar]
  12. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  13. Schmidt, N. J. & Emmons, R. W. (editors) ( 1989; ). Diagnostic Procedures for Viral, Rickettsial and Chlamydial Infections, 6th edn. Washington, DC: American Public Health Association.
  14. Simmons, J. H., Purdy, G. A., Franklin, C. L., Trottier, P., Churchill, A. E., Russell, R. J., Besch-Williford, C. L. & Riley, L. K. ( 2002; ). Characterization of a novel parainfluenza virus, caviid parainfluenza virus 3, from laboratory guinea pigs (Cavia porcellus). Comp Med 52, 548–554.
    [Google Scholar]
  15. Welch, B. G., Snow, E. J., Jr, Hegner, J. R., Adams, S. R., Jr & Quist, K. D. ( 1977; ). Development of a guinea pig colony free of complement-fixing antibodies to parainfluenza virus. Lab Anim Sci 27, 976–979.
    [Google Scholar]
  16. Wyde, P. R., Amborose, M. W., Meyer, H. L. & Gilbert, B. E. ( 1990; ). Toxicity and antiviral activity of LY253963 against respiratory syncytial and parainfluenza type 3 viruses in tissue culture and in cotton rats. Antiviral Res 14, 237–247.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.80666-0
Loading
/content/journal/jgv/10.1099/vir.0.80666-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error