1887

Abstract

Subcellular localization of the cysteine-rich b protein was studied by using different approaches. In infected tissue, b was detected mainly in the P30 fraction as monomers, dimers and oligomers. Green fluorescent protein-fused b was found to localize in punctate bodies in the cytoplasm. Colocalization with marker proteins demonstrated that these bodies represent peroxisomes. Immunoelectron microscopy revealed that b was localized in the peroxisomal matrix and that localization of b in peroxisomes required the C-terminal signal tripeptide SKL. An SKL-deletion mutant exhibited a diffuse localization, but retained the protein's ability to suppress RNA silencing, determine infection phenotype and support virus systemic spread. These data indicate that b functions are not associated with the protein's localization to peroxisomes.

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2005-02-01
2019-12-10
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