1887

Abstract

Glycoprotein H (gH) homologues are found in all members of the herpes virus family, and gH is one of the virion envelope glycoproteins that is essential for virus entry. In this study, a recombinant soluble form of Herpes simplex virus type 1 (HSV-1) gH, in which the ectodomain is fused to the Fc-binding region of IgG, has been generated. This was expressed in mammalian cells together with gL and the resulting gHFc–gL heterodimer was purified using Protein A Sepharose. Low-affinity cell binding assays showed that gHFc–gL bound specifically to Vero cells and mutation of a potential integrin-binding motif, Arg-Gly-Asp (RGD), in gH abolished binding. CHO cells failed to bind in this assay. However, CHO cells expressing the human v3 integrin bound efficiently to gHFc–gL, suggesting that HSV-1 gH can bind to cells using v3 integrins and that this binding is mediated by the RGD motif in the gH ectodomain.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.80567-0
2005-01-01
2021-10-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/86/1/vir860007.html?itemId=/content/journal/jgv/10.1099/vir.0.80567-0&mimeType=html&fmt=ahah

References

  1. Akula S. M., Naranatt P. P., Walia N. S., Wang F. Z., Fegley B., Chandran B. 2003; Kaposi's sarcoma-associated herpesvirus (human herpesvirus 8) infection of human fibroblast cells occurs through endocytosis. J Virol 77:7978–7990 [CrossRef]
    [Google Scholar]
  2. Buckmaster E. A., Gompels U., Minson A. C. 1984; Characterisation and physical mapping of an HSV-1 glycoprotein of approximately 115×103 molecular weight. Virology 139:408–413 [CrossRef]
    [Google Scholar]
  3. Cai W. H., Gu B., Person S. 1988; Role of glycoprotein B of herpes simplex virus type 1 in viral entry and cell fusion. J Virol 62:2596–2604
    [Google Scholar]
  4. Carfi A., Willis S. H., Whitbeck J. C., Krummenacher C., Cohen G. H., Eisenberg R. J., Wiley D. C. 2001; Herpes simplex virus glycoprotein D bound to the human receptor HveA. Mol Cell 8:169–179 [CrossRef]
    [Google Scholar]
  5. Cheshenko N., Del Rosario B., Woda C., Marcellino D., Satlin L. M., Herold B. C. 2003; Herpes simplex virus triggers activation of calcium-signaling pathways. J Cell Biol 163:283–293 [CrossRef]
    [Google Scholar]
  6. Diamond M. S., Staunton D. E., de Fougerolles A. R., Stacker S. A., Garcia-Aguilar J., Hibbs M. L., Springer T. A. 1990; ICAM-1 (CD54): a counter-receptor for Mac-1 (CD11b/CD18). J Cell Biol 111:3129–3139 [CrossRef]
    [Google Scholar]
  7. Forrester A., Farrell H., Wilkinson G., Kaye J., Davis-Poynter N., Minson T. 1992; Construction and properties of a mutant of herpes simplex virus type 1 with glycoprotein H coding sequences deleted. J Virol 66:341–348
    [Google Scholar]
  8. Galdiero M., Whiteley A., Bruun B., Bell S., Minson T., Browne H. 1997; Site-directed and linker insertion mutagenesis of herpes simplex virus type 1 glycoprotein H. J Virol 71:2163–2170
    [Google Scholar]
  9. Giancotti F. G., Ruoslahti E. 1999; Integrin signaling. Science 285:1028–1032 [CrossRef]
    [Google Scholar]
  10. Herold B. C., WuDunn D., Soltys N., Spear P. G. 1991; Glycoprotein C of herpes simplex virus type 1 plays a principal role in the adsorption of virus to cells and in infectivity. J Virol 65:1090–1098
    [Google Scholar]
  11. Hutchinson L., Browne H., Wargent V., Davis-Poynter N., Primorac S., Goldsmith K., Minson A. C., Johnson D. C. 1992; A novel herpes simplex virus glycoprotein, gL, forms a complex with glycoprotein H (gH) and affects normal folding and cell surface expression of gH. J Virol 66:2240–2250
    [Google Scholar]
  12. Israel B. A., Schultz K. T., Murphy C. J. 1998; Lack of detectable interaction between HSV-1 and integrins or tachykinins. Intervirology 41:132–134 [CrossRef]
    [Google Scholar]
  13. Keay S., Baldwin B. 1991; Anti-idiotype antibodies that mimic gp86 of human cytomegalovirus inhibit viral fusion but not attachment. J Virol 65:5124–5128
    [Google Scholar]
  14. Li Q., Spriggs M. K., Kovats S., Turk S. M., Comeau M. R., Nepom B., Hutt-Fletcher L. M. 1997; Epstein-Barr virus uses HLA class II as a cofactor for infection of B lymphocytes. J Virol 71:4657–4662
    [Google Scholar]
  15. Ligas M. W., Johnson D. C. 1988; A herpes simplex virus mutant in which glycoprotein D sequences are replaced by beta-galactosidase sequences binds to but is unable to penetrate into cells. J Virol 62:1486–1494
    [Google Scholar]
  16. Mekrache M., Kieffer N., Baruch D. 2002; Activation of recombinant α IIb β 3 expressed in Chinese hamster ovary cells exposes different binding sites for fibrinogen or von Willebrand factor: evidence using monoclonal antibodies to α IIb β 3. Br J Haematol 116:636–644 [CrossRef]
    [Google Scholar]
  17. Montgomery R. I., Warner M. S., Lum B. J., Spear P. J. 1996; Herpes simplex virus-1 entry into cells mediated by a novel member of the TNF/NGF receptor family. Cell 87:427–436 [CrossRef]
    [Google Scholar]
  18. Mori Y., Yang X., Akkapaiboon P., Okuno T., Yamanishi K. 2003; Human herpesvirus 6 variant A glycoprotein H-glycoprotein L-glycoprotein Q complex associates with human CD46. J Virol 77:4992–4999 [CrossRef]
    [Google Scholar]
  19. Muggeridge M. I. 2000; Characterization of cell-cell fusion mediated by herpes simplex virus 2 glycoproteins gB, gD, gH and gL in transfected cells. J Gen Virol 81:2017–2027
    [Google Scholar]
  20. Pietropaolo R. L., Compton T. 1997; Direct interaction between human cytomegalovirus glycoprotein B and cellular annexin II. J Virol 71:9803–9807
    [Google Scholar]
  21. Santoro F., Greenstone H. L., Insinga A., Liszewski M. K., Atkinson J. P., Lusso P., Berger E. A. 2003; Interaction of glycoprotein H of human herpesvirus 6 with the cellular receptor CD46. J Biol Chem 278:25964–25969 [CrossRef]
    [Google Scholar]
  22. Scanlan P. M., Tiwari V., Bommireddy S., Shukla D. 2003; Cellular expression of gH confers resistance to herpes simplex virus type-1 entry. Virology 312:14–24 [CrossRef]
    [Google Scholar]
  23. Spear P. G. 2004; Herpes simplex virus: receptors and ligands for cell entry. Cell Microbiol 6:401–410 [CrossRef]
    [Google Scholar]
  24. Turner A., Bruun B., Minson T., Browne H. 1998; Glycoproteins gB, gD and gHgL of herpes simplex virus type 1 are necessary and sufficient to mediate membrane fusion in a Cos cell transfection system. J Virol 72:873–875
    [Google Scholar]
  25. Wang F. Z., Akula S. M., Sharma-Walia N., Zeng L., Chandran B. 2003a; Human herpesvirus 8 envelope glycoprotein B mediates cell adhesion via its RGD sequence. J Virol 77:3131–3147 [CrossRef]
    [Google Scholar]
  26. Wang X., Huong S. M., Chiu M. L., Raab-Traub N., Huang E. S. 2003b; Epidermal growth factor receptor is a cellular receptor for human cytomegalovirus. Nature 424:456–461 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.80567-0
Loading
/content/journal/jgv/10.1099/vir.0.80567-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error