%0 Journal Article %A Hausmann, Jürgen %A Baur, Karen %A Engelhardt, Karin R. %A Fischer, Timo %A Rziha, Hanns-Joachim %A Staeheli, Peter %T Vaccine-induced protection against Borna disease in wild-type and perforin-deficient mice %D 2005 %J Journal of General Virology, %V 86 %N 2 %P 399-403 %@ 1465-2099 %R https://doi.org/10.1099/vir.0.80566-0 %I Microbiology Society, %X Borna disease virus (BDV) can persistently infect the central nervous system and induce CD8+ T-cell-mediated neurological disease in MRL mice. To determine whether specific immune priming would prevent disease, a prime–boost immunization protocol was established in which intramuscular injection of a recombinant parapoxvirus expressing BDV nucleoprotein (BDV-N) was followed by intraperitoneal infection with vaccinia virus expressing BDV-N. Immunized wild-type and perforin-deficient mice remained healthy after intracerebral infection with BDV and contained almost no virus in the brain at 5 weeks post-challenge. Immunization failed to induce resistance against BDV in mice lacking mature CD8+ T cells. Immunization of perforin-deficient mice with a poxvirus vector expressing mutant BDV-N lacking the known CD8+ T-cell epitope did not efficiently block multiplication of BDV in the brain and did not prevent neurological disease, indicating that vaccine-induced immunity to BDV in wild-type and perforin-deficient mice resulted from the action of CD8+ T cells. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.80566-0