1887

Abstract

Koala retrovirus (KoRV) is a newly described endogenous retrovirus and is unusual in that inserts comprise a full-length replication competent genome. As koalas are known to suffer from an extremely high incidence of leukaemia/lymphoma, the association between this retrovirus and disease in koalas was examined. Using quantitative real-time reverse transcriptase PCR it was demonstrated that KoRV RNA levels in plasma are significantly increased in animals suffering from leukaemia or lymphoma when compared with healthy animals. Increased levels of KoRV were also seen for animals with clinical chlamydiosis. A significant positive association between viral RNA levels and age was also demonstrated. Real-time PCR demonstrated as much as 5 log variation in KoRV proviral DNA levels in genomic DNA extracted from whole blood from different animals. Taken together these data indicate that KoRV is an active endogenous retrovirus and suggests that it may be causally linked to neoplastic disease in koalas.

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2005-03-01
2019-11-18
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References

  1. Akiyoshi, D. E., Denaro, M., Zhu, H., Greenstein, J. L., Banerjee, P. & Fishman, J. A. ( 1988; ). Identification of a full-length cDNA for an endogenous retrovirus of miniature swine. J Virol 72, 4503–4507.
    [Google Scholar]
  2. Baillie, G. J. & Wilkins, R. J. ( 2001; ). Endogenous type D retrovirus in a marsupial, the common brushtail possum (Trichosurus vulpeca). J Virol 75, 2499–2507.[CrossRef]
    [Google Scholar]
  3. Bartman, T., Murasko, D. M. & Blank, K. ( 1995; ). A replication-competent, endogenous retrovirus from an aged DBA/2 mouse contains the complete env from Emv-3 and a novel gag partially related to AKT-8. J Virol 69, 3244–3228.
    [Google Scholar]
  4. Bock, M. & Stoye, J. P. ( 2000; ). Endogenous retroviruses and the human germline. Curr Opin Genet Dev 10, 651–655.[CrossRef]
    [Google Scholar]
  5. Boeke, J. D. & Stoye, J. P. ( 1997; ). Retrotransposons, endogenous retroviruses, and the evolution of retroelements. In Retroviruses, pp. 343–346. Edited by J. M. Coffin, S. H. Hughes & H. E. Varmus. Cold Spring Harbor: Cold Spring Harbor Laboratory.
  6. Booth, R. J. & Blanshard, W. H. ( 1999; ). Diseases of koalas. In Zoo and Wild Animal Medicine. Edited by M. E. Fowler & R. E. Miller. Philadelphia: W. B. Saunders.
  7. Brown, A. S., Girjes, A. A., Lavin, M. F., Timms, P. & Woolcock, J. B. ( 1987; ). Chlamydial disease in koalas. Aust Vet J 64, 346–350.[CrossRef]
    [Google Scholar]
  8. Canfield, P. J. ( 1991; ). A survey of koala road kills in New South Wales. J Wildl Dis 27, 657–660.[CrossRef]
    [Google Scholar]
  9. Canfield, P. J., Sabine, J. M. & Love, D. N. ( 1988; ). Virus particles associated with leukaemia in a koala. Aust Vet J 65, 327–328.[CrossRef]
    [Google Scholar]
  10. Canfield, P. J., O'Neill, M. E. & Smith, E. F. ( 1989; ). Haematological and biochemical reference values for the koala (Phascolarctos cinereus). Aust Vet J 66, 324–326.[CrossRef]
    [Google Scholar]
  11. Ceccarelli, A. V. & Rozengurt, N. ( 2002; ). Outbreak of hind limb paralysis in young CFW Swiss Webster mice. Comp Med 52, 171–175.
    [Google Scholar]
  12. Cockram, F. A. & Jackson, A. R. ( 1981; ). Keratoconjunctivitis of the koala, Phascolarctos cinereus, caused by Chlamydia psittaci. J Wildl Dis 17, 497–504.[CrossRef]
    [Google Scholar]
  13. Contini, C., Fainardi, F., Seraceni, S., Granieri, F., Castellazzi, M. & Cultrera, R. ( 2003; ). Molecular identification and antibody testing of Chlamydophila pneumoniae in a subgroup of patients with HIV-associated dementia complex. Preliminary results. J Neuroimmunol 136, 172–177.[CrossRef]
    [Google Scholar]
  14. Couto, C. G. ( 1992; ). Diagnosis and management of retroviral diseases in cats. In Essentials of Small Animal Internal Medicine, pp. 954–959. Edited by R. W. Nelson & C. G. Couto. St Louis: Mosby – Year Book.
  15. Depil, S., Roche, C., Dussart, P. & Prin, L. ( 2002; ). Expression of a human endogenous retrovirus, HERV-K, in the blood cells of leukemia patients. Leukemia 16, 254–259.[CrossRef]
    [Google Scholar]
  16. Gaubatz, J. W., Acrement, B. & Cutler, R. G. ( 1991; ). Gene expression of an endogenous retrovirus-like element during murine development and aging. Mech Ageing Dev 57, 71–85.[CrossRef]
    [Google Scholar]
  17. Hanger, J. J., Bromham, L. D., McKee, J. J., O'Brien, T. M. & Robinson, W. F. ( 2000; ). The nucleotide sequence of koala (Phascolarctos cinereus) retrovirus: a novel type C endogenous virus related to Gibbon ape leukemia virus. J Virol 74, 4264–4272.[CrossRef]
    [Google Scholar]
  18. Hoover, E. A. & Mullins, J. I. ( 1991; ). Feline leukemia virus infection and diseases. J Am Vet Med Assoc 199, 1287–1297.
    [Google Scholar]
  19. Lee, D. H., Li, L., Andrus, L. & Prince, A. M. ( 2002; ). Stabilized viral nucleic acids in plasma as an alternative shipping method for NAT. Transfusion 42, 409–413.[CrossRef]
    [Google Scholar]
  20. Martin, J., Hernoiu, E., Cook, J., O'Neill, R. W. & Tristem, M. ( 1999; ). Interclass transmission and phyletic host tracking in murine leukemia virus-related retoviruses. J Virol 73, 2442–2449.
    [Google Scholar]
  21. O'Dair, H. A., Hopper, C. D., Gruffydd-Jones, T. J., Harbour, D. A. & Waters, L. ( 1994; ). Clinical aspects of Chlamydia psittaci infection in cats infected with feline immunodeficiency virus. Vet Rec 134, 365–368.[CrossRef]
    [Google Scholar]
  22. Ono, T., Shinya, K., Uehara, Y. & Okada, S. ( 1989; ). Endogenous virus genomes become hypomethylated tissue – specifically during ageing process of C57BL mice. Mech Ageing Dev 50, 27–36.[CrossRef]
    [Google Scholar]
  23. Patzke, S., Lindeskog, M., Munthe, E. & Aasheim, H. C. ( 2002; ). Characterization of a novel human endogenous retrovirus, HERV-H/F, expressed in human leukemia cell lines. Virology 303, 164–173.[CrossRef]
    [Google Scholar]
  24. Perron, H., Jouvin-Marche, E., Michel, M. & 13 other authors ( 2001; ). Multiple sclerosis retrovirus particles and recombinant envelope trigger an abnormal immune response in vitro, by inducing polyclonal VB16 T-lymphocyte activation. Virology 287, 321–332.[CrossRef]
    [Google Scholar]
  25. Rosenburg, N. & Jolicouer, P. ( 1997; ). Retroviral pathogenesis. In Retroviruses, pp. 457–587. Edited by J. M. Coffin, S. H. Hughes & H. E. Varmus. Cold Spring Harbor: Cold Spring Harbor Laboratory.
  26. Sacco, M. A., Howes, K. & Venugopal, K. ( 2001; ). Intact EAV-HP endogenous retrovirus in Sonnerat's jungle fowl. J Virol 75, 2029–2032.[CrossRef]
    [Google Scholar]
  27. Vogt, V. M. ( 1997; ). Retroviral virions and genomes. In Retroviruses, pp. 27–70. Edited by J. M. Coffin, S. H. Hughes & H. E. Varmus. Cold Spring Harbor: Cold Spring Harbor Laboratory.
  28. Wada, Y., Tsukada, M., Kamiyama, S. & Koizumi, A. ( 1993; ). Retroviral gene expression as a possible biomarker of ageing. Int Arch Occup Environ Health 65, S235–S239.[CrossRef]
    [Google Scholar]
  29. Wang-Johanning, F., Frost, A. R., Jian, B., Epp, L., Lu, D. W. & Johanning, G. L. ( 2003; ). Quantitation of HERV-K env gene expression and splicing in human breast cancer. Oncogene 22, 1528–1535.[CrossRef]
    [Google Scholar]
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