1887

Abstract

Severe acute respiratory syndrome coronavirus (SARS-CoV) has been identified as the aetiological agent of SARS. Thus, vaccination against SARS-CoV may represent an effective approach towards controlling SARS. The nucleocapsid (N) protein is thought to play a role in induction of cell-mediated immunity to SARS-CoV and thus it is important to characterize this protein. In the present study, an E1/partially E3-deleted, replication-defective human adenovirus 5 (Ad5) vector (Ad5-N-V) expressing the SARS-CoV N protein was constructed. The N protein, expressed by Ad5-N-V, was of the expected molecular mass of 50 kDa and was phosphorylated. Vaccination of C57BL/6 mice with Ad5-N-V generated potent SARS-CoV-specific humoral and T cell-mediated immune responses. These results show that Ad5-N-V may potentially be used as a SARS-CoV vaccine.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.80530-0
2005-01-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/86/1/vir860211.html?itemId=/content/journal/jgv/10.1099/vir.0.80530-0&mimeType=html&fmt=ahah

References

  1. Chang M.-S., Lu Y.-T., Ho S.-T. 11 other authors 2004; Antibody detection of SARS-CoV spike and nucleocapsid protein. Biochem Biophys Res Commun 314:931–936 [CrossRef]
    [Google Scholar]
  2. Christian M. D., Poutanen S. M., Loutfy M. R., Muller M. P., Low D. E. 2004; Severe acute respiratory syndrome. Clin Infect Dis 38:1420–1427 [CrossRef]
    [Google Scholar]
  3. Gao W., Tamin A., Soloff A., D'Aiuto L., Nwanegbo E., Robbins P. D., Bellini W. J., Barratt-Boyes S., Gambotto A. 2003; Effects of a SARS-associated coronavirus vaccine in monkeys. Lancet 362:1895–1896 [CrossRef]
    [Google Scholar]
  4. He R., Leeson A., Andonov A. 8 other authors 2003; Activation of AP-1 signal transduction pathway by SARS coronavirus nucleocapsid protein. Biochem Biophys Res Commun 311:870–876 [CrossRef]
    [Google Scholar]
  5. He R., Dobie F., Ballantine M. 11 other authors 2004; Analysis of multimerization of the SARS coronavirus nucleocapsid protein. Biochem Biophys Res Commun 316:476–483 [CrossRef]
    [Google Scholar]
  6. Huang Q., Yu L., Petros A. M. 7 other authors 2004; Structure of the N-terminal RNA-binding domain of the SARS CoV nucleocapsid protein. Biochemistry 43:6059–6063 [CrossRef]
    [Google Scholar]
  7. Kim T. W., Lee J. H., Hung C.-F. 9 other authors 2004; Generation and characterization of DNA vaccines targeting the nucleocapsid protein of severe acute respiratory syndrome coronavirus. J Virol 78:4638–4645 [CrossRef]
    [Google Scholar]
  8. King B., Brian D. A. 1982; Bovine coronavirus structural proteins. J Virol 42:700–707
    [Google Scholar]
  9. Kuo L., Masters P. S. 2002; Genetic evidence for a structural interaction between the carboxy termini of the membrane and nucleocapsid proteins of mouse hepatitis virus. J Virol 76:4987–4999 [CrossRef]
    [Google Scholar]
  10. Leung D. T., Tam F. C., Ma C. H., Chan P. K., Cheung J. L., Niu H., Tam J. S., Lim P. L. 2004; Antibody response of patients with severe acute respiratory syndrome (SARS) targets the viral nucleocapsid. J Infect Dis 190:379–386 [CrossRef]
    [Google Scholar]
  11. Liu C., Kokuho T., Kubota T., Watanabe S., Inumaru S., Yokomizo Y., Onodera T. 2001; DNA mediated immunization with encoding the nucleoprotein gene of porcine transmissible gastroenteritis virus. Virus Res 80:75–82 [CrossRef]
    [Google Scholar]
  12. Lomniczi B., Morser J. 1981; Polypeptides of infectious bronchitis virus. I. Polypeptides of the virion. J Gen Virol 55:155–164 [CrossRef]
    [Google Scholar]
  13. Marra M. A., Jones S. J. M., Astell C. R. 56 other authors 2003; The genome sequence of the SARS-associated coronavirus. Science 300:1399–1404 [CrossRef]
    [Google Scholar]
  14. Mohandas D. V., Dales S. 1991; Endosomal association of a protein phosphatase with high dephosphorylating activity against a coronavirus nucleocapsid protein. FEBS Lett 282:419–424 [CrossRef]
    [Google Scholar]
  15. Narayanan K., Chen C.-J., Maeda J., Makino S. 2003; Nucleocapsid-independent specific viral RNA packaging via viral envelope protein and viral RNA signal. J Virol 77:2922–2927 [CrossRef]
    [Google Scholar]
  16. Parker M. M., Masters P. S. 1990; Sequence comparison of the N genes of five strains of the coronavirus mouse hepatitis virus suggests a three domain structure for the nucleocapsid protein. Virology 179:463–468 [CrossRef]
    [Google Scholar]
  17. Rota P. A., Oberste M. S., Monroe S. S. 32 other authors 2003; Characterization of a novel coronavirus associated with severe acute respiratory syndrome. Science 300:1394–1399 [CrossRef]
    [Google Scholar]
  18. Seo S. H., Wang L., Smith R., Collisson E. W. 1997; The carboxyl-terminal 120-residue polypeptide of infectious bronchitis virus nucleocapsid induces cytotoxic T lymphocytes and protects chickens from acute infection. J Virol 71:7889–7894
    [Google Scholar]
  19. Shiver J. W., Fu T.-M., Chen L. 49 other authors; 2002; Replication-incompetent adenoviral vaccine vector elicits effective anti-immunodeficiency-virus immunity. Nature 415:331–335 [CrossRef]
    [Google Scholar]
  20. Stohlman S. A., Lai M. M. 1979; Phosphoproteins of murine hepatitis viruses. J Virol 32:672–675
    [Google Scholar]
  21. Sullivan N. J., Sanchez A., Rollin P. E., Yang Z.-Y., Nabel G. J. 2000; Development of a preventive vaccine for Ebola virus infection in primates. Nature 408:605–609 [CrossRef]
    [Google Scholar]
  22. Sullivan N. J., Geisbert T. W., Geisbert J. B., Xu L., Yang Z.-Y., Roederer M., Koup R. A., Jahrling P. B., Nabel G. J. 2003; Accelerated vaccination for Ebola virus haemorrhagic fever in non-human primates. Nature 424:681–684 [CrossRef]
    [Google Scholar]
  23. Surjit M., Liu B., Kumar P., Chow V. T. K., Lal S. K. 2004; The nucleocapsid protein of the SARS coronavirus is capable of self-association through a C-terminal 209 amino acid interaction domain. Biochem Biophys Res Commun 317:1030–1036 [CrossRef]
    [Google Scholar]
  24. Wilbur S. M., Nelson G. W., Lai M. M., McMillan M., Stohlman S. A. 1986; Phosphorylation of the mouse hepatitis virus nucleocapsid protein. Biochem Biophys Res Commun 141:7–12 [CrossRef]
    [Google Scholar]
  25. Wilson G. A., Mohandas D. V., Dales S. 1990; In vivo and in vitro models of demyelinating disease. Possible relationship between induction of regulatory subunit from cAMP dependent protein kinases and inhibition of JHMV replication in cultured oligodendrocytes. Adv Exp Med Biol 276:261–266
    [Google Scholar]
  26. Ying W., Hao Y., Zhang Y. 33 other authors 2004; Proteomic analysis on structural proteins of severe acute respiratory syndrome coronavirus. Proteomics 4:492–504 [CrossRef]
    [Google Scholar]
  27. Zhu M.-S., Pan Y., Chen H.-Q., Shen Y., Wang X.-C., Sun Y.-J., Tao K.-H. 2004; Induction of SARS-nucleoprotein-specific immune response by use of DNA vaccine. Immunol Lett 92:237–243 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.80530-0
Loading
/content/journal/jgv/10.1099/vir.0.80530-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error