RT Journal Article SR Electronic(1) A1 Isogai, Masamichi A1 Yoshikawa, NobuyukiYR 2005 T1 Mapping the RNA-binding domain on the Apple chlorotic leaf spot virus movement protein JF Journal of General Virology, VO 86 IS 1 SP 225 OP 229 DO https://doi.org/10.1099/vir.0.80493-0 PB Microbiology Society, SN 1465-2099, AB The RNA-binding properties of the cell-to-cell movement protein (MP) of Apple chlorotic leaf spot virus were analysed. MP was expressed in Escherichia coli and was used in UV-crosslinking analysis, using a digoxigenin–UTP-labelled RNA probe and gel-retardation analysis. The analyses demonstrated that MP bound cooperatively to single-stranded RNA (ssRNA). When analysed for NaCl dependence of the RNA-binding activity, the majority of the MP could bind ssRNA even in binding buffer with 1 M NaCl. Furthermore, competition binding experiments showed that the MP bound preferentially to ssRNA and single-stranded DNA without sequence specificity. MP deletion mutants were used to identify the RNA-binding domain by UV-crosslinking analysis. Amino acid residues 82–126 and 127–287 potentially contain two independently active, single-stranded nucleic acid-binding domains., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.80493-0