%0 Journal Article %A Isogai, Masamichi %A Yoshikawa, Nobuyuki %T Mapping the RNA-binding domain on the Apple chlorotic leaf spot virus movement protein %D 2005 %J Journal of General Virology, %V 86 %N 1 %P 225-229 %@ 1465-2099 %R https://doi.org/10.1099/vir.0.80493-0 %I Microbiology Society, %X The RNA-binding properties of the cell-to-cell movement protein (MP) of Apple chlorotic leaf spot virus were analysed. MP was expressed in Escherichia coli and was used in UV-crosslinking analysis, using a digoxigenin–UTP-labelled RNA probe and gel-retardation analysis. The analyses demonstrated that MP bound cooperatively to single-stranded RNA (ssRNA). When analysed for NaCl dependence of the RNA-binding activity, the majority of the MP could bind ssRNA even in binding buffer with 1 M NaCl. Furthermore, competition binding experiments showed that the MP bound preferentially to ssRNA and single-stranded DNA without sequence specificity. MP deletion mutants were used to identify the RNA-binding domain by UV-crosslinking analysis. Amino acid residues 82–126 and 127–287 potentially contain two independently active, single-stranded nucleic acid-binding domains. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.80493-0