1887

Abstract

Molecular methods have enabled the rapid identification of new enterovirus (EV) serotypes that would have been untypable using existing neutralizing antisera. Nineteen strains of four new EV types termed EV76 (11 isolates), EV89 (two isolates), EV90 (four isolates) and EV91 (two isolates), isolated from clinical specimens from patients in France (one isolate) and Bangladesh (18 isolates), are described. Nucleotide sequences encoding the VP1 capsid protein (882–888 nt) are less than 65 % identical to the homologous sequences of the recognized human EV serotypes, but within each group the sequences are more than 78 % identical. The deduced amino acid sequences of the complete capsid (P1) region are more than 94 % identical within type but less than 76 % identical to those of the recognized serotypes. For both VP1 and P1, the 19 isolates are monophyletic by type with respect to all other EV serotypes. Using the proposed molecular typing scheme, these data support their identification as four new types within the species (HEV-A). In almost all cases, the VP1 sequences were more similar to those of some simian EVs than to the human EVs. Partial 3D sequences of all 19 isolates also clustered within HEV-A; they were monophyletic as a group, but not by type, suggesting that recombination has occurred among viruses of these four types. Partial 3D sequences were more closely related to those of simian EVs than to human viruses in HEV-A. These results suggest that the four new types may represent a new subgroup within HEV-A, in addition to the existing human and simian subgroups.

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2005-02-01
2019-09-20
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References

  1. Andersson, P., Edman, K. & Lindberg, A. M. ( 2002; ). Molecular analysis of the echovirus 18 prototype: evidence of interserotypic recombination with echovirus 9. Virus Res 85, 71–83.[CrossRef]
    [Google Scholar]
  2. Bailly, J. L., Cardoso, M.-C., Labbé, A. & Peigue-Lafeuille, H. ( 2004; ). Isolation and identification of an enterovirus 77 recovered from a refugee child from Kosovo, and characterization of the complete virus genome. Virus Res 99, 147–155.[CrossRef]
    [Google Scholar]
  3. Belliot, G., Laveran, H. & Monroe, S. S. ( 1997; ). Outbreak of gastroenteritis in military recruits associated with serotype 3 astrovirus infection. J Med Virol 51, 101–106.[CrossRef]
    [Google Scholar]
  4. Brown, B. A. & Pallansch, M. A. ( 1995; ). Complete nucleotide sequence of enterovirus 71 is distinct from poliovirus. Virus Res 39, 195–205.[CrossRef]
    [Google Scholar]
  5. Brown, B. A., Oberste, M. S., Maher, K. & Pallansch, M. A. ( 2003; ). Complete genomic sequencing shows that polioviruses and members of human enterovirus species C are closely related in the noncapsid coding region. J Virol 77, 8973–8984.[CrossRef]
    [Google Scholar]
  6. Chua, B. H., McMinn, P. C., Lam, S. K. & Chua, K. B. ( 2001; ). Comparison of the complete nucleotide sequences of echovirus 7 strain UMMC and the prototype (Wallace) strain demonstrates significant genetic drift over time. J Gen Virol 82, 2629–2639.
    [Google Scholar]
  7. Heberling, R. L. & Cheever, F. S. ( 1965; ). Some characteristics of the simian enteroviruses. Am J Epidemiol 81, 106–123.
    [Google Scholar]
  8. Hoffert, W. R., Bates, M. E. & Cheever, F. S. ( 1958; ). Study of enteric viruses of simian origin. Am J Hyg 68, 15–30.
    [Google Scholar]
  9. Hull, R. N., Minner, J. R. & Mascoli, C. C. ( 1958; ). New viral agents recovered from tissue cultures of monkey kidney cells. III. Recovery of additional agents both from cultures of monkey tissues and directly from tissues and excreta. Am J Hyg 68, 31–44.
    [Google Scholar]
  10. Kennett, M. L., Birch, C. J., Lewis, F. A., Yung, A. P., Locarnini, S. A. & Gust, I. D. ( 1974; ). Enterovirus type 71 infection in Melbourne. Bull W H O 51, 609–615.
    [Google Scholar]
  11. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  12. King, A. M. Q., Brown, F., Christian, P. & 8 other authors ( 2000; ). Picornaviridae. In Virus Taxonomy. Seventh Report of the International Committee on Taxonomy of Viruses, pp. 657–678. Edited by M. H. V. van Regenmortel, C. M. Fauquet, D. H. L. Bishop, E. B. Carstens, M. K. Estes, S. M. Lemon, J. Maniloff, M. A. Mayo, D. J. McGeoch, C. R. Pringle & R. B. Wickner. San Diego: Academic Press.
  13. Kumar, S., Tamura, K., Jakobsen, I. B. & Nei, M. ( 2001; ). mega2: molecular evolutionary genetics analysis software. Bioinformatics 17, 1244–1245.[CrossRef]
    [Google Scholar]
  14. Lindberg, A. M., Andersson, P., Savolainen, C., Mulders, M. N. & Hovi, T. ( 2003; ). Evolution of the genome of Human enterovirus B: incongruence between phylogenies of the VP1 and 3CD regions indicates frequent recombination within the species. J Gen Virol 84, 1223–1235.[CrossRef]
    [Google Scholar]
  15. Liu, H.-M., Zheng, D.-P., Zhang, L.-B., Oberste, M. S., Pallansch, M. A. & Kew, O. M. ( 2000; ). Molecular evolution of a type 1 wild-vaccine poliovirus recombinant during widespread circulation in China. J Virol 74, 11153–11161.[CrossRef]
    [Google Scholar]
  16. Liu, H.-M., Zheng, D.-P., Zhang, L.-B., Oberste, M. S., Kew, O. M. & Pallansch, M. A. ( 2003; ). Serial recombination during circulation of type 1 wild-vaccine recombinant polioviruses in China. J Virol 77, 10994–11005.[CrossRef]
    [Google Scholar]
  17. Lukashev, A. N., Lashkevich, V. A., Ivanova, O. E., Koroleva, G. A., Hinkkanen, A. E. & Ilonen, J. ( 2003; ). Recombination in circulating enteroviruses. J Virol 77, 10423–10431.[CrossRef]
    [Google Scholar]
  18. Lukashev, A. N., Lashkevich, V. A., Koroleva, G. A., Ilonen, J. & Hinkkanen, A. E. ( 2004; ). Recombination in uveitis-causing enterovirus strains. J Gen Virol 85, 463–470.[CrossRef]
    [Google Scholar]
  19. Muckelbauer, J. K., Kremer, M., Minor, I., Diana, G., Dutko, F. J., Groarke, J., Pevear, D. C. & Rossman, M. G. ( 1995; ). The structure of coxsackievirus B3 at 3·5 Å resolution. Structure 3, 653–667.[CrossRef]
    [Google Scholar]
  20. Norder, H., Bjerregaard, L., Magnius, L., Lina, B., Aymard, M. & Chomel, J.-J. ( 2003; ). Sequencing of ‘untypable’ enteroviruses reveals two new types, EV-77 and EV-78, within human enterovirus type B and substitutions in the BC loop of the VP1 protein for known types. J Gen Virol 84, 827–836.[CrossRef]
    [Google Scholar]
  21. Oberste, M. S., Maher, K., Kilpatrick, D. R., Flemister, M. R., Brown, B. A. & Pallansch, M. A. ( 1999a; ). Typing of human enteroviruses by partial sequencing of VP1. J Clin Microbiol 37, 1288–1293.
    [Google Scholar]
  22. Oberste, M. S., Maher, K., Kilpatrick, D. R. & Pallansch, M. A. ( 1999b; ). Molecular evolution of the human enteroviruses: correlation of serotype with VP1 sequence and application to picornavirus classification. J Virol 73, 1941–1948.
    [Google Scholar]
  23. Oberste, M. S., Maher, K., Flemister, M. R., Marchetti, G., Kilpatrick, D. R. & Pallansch, M. A. ( 2000; ). Comparison of classic and molecular approaches for the identification of untypeable enteroviruses. J Clin Microbiol 38, 1170–1174.
    [Google Scholar]
  24. Oberste, M. S., Schnurr, D., Maher, K., al-Busaidy, S. & Pallansch, M. A. ( 2001; ). Molecular identification of new picornaviruses and characterization of a proposed enterovirus 73 serotype. J Gen Virol 82, 409–416.
    [Google Scholar]
  25. Oberste, M. S., Maher, K. & Pallansch, M. A. ( 2002; ). Molecular phylogeny and classification of the simian picornaviruses. J Virol 76, 1244–1251.[CrossRef]
    [Google Scholar]
  26. Oberste, M. S., Nix, W. A., Maher, K. & Pallansch, M. A. ( 2003; ). Improved molecular identification of enteroviruses by RT-PCR and amplicon sequencing. J Clin Virol 26, 375–377.[CrossRef]
    [Google Scholar]
  27. Oberste, M. S., Maher, K. & Pallansch, M. ( 2004a; ). Evidence for frequent recombination within Human enterovirus B based on complete genomic sequences of all 37 serotypes. J Virol 78, 855–867.[CrossRef]
    [Google Scholar]
  28. Oberste, M. S., Maher, K., Schnurr, D. & 9 other authors ( 2004b; ). Enterovirus 68 is associated with respiratory illness and shares features with both the enteroviruses and the rhinoviruses. J Gen Virol 85, 2577–2584.[CrossRef]
    [Google Scholar]
  29. Oberste, M. S., Michele, S. M., Maher, K. & 11 other authors ( 2004c; ). Molecular identification and characterization of two proposed new enterovirus serotypes, EV74 and EV75. J Gen Virol 85, 3205–3212.[CrossRef]
    [Google Scholar]
  30. Oberste, M. S., Peñaranda, S., Maher, K. & Pallansch, M. A. ( 2004d; ). Complete genome sequences of members of the species Human enterovirus A. J Gen Virol 85, 1597–1607.[CrossRef]
    [Google Scholar]
  31. Oberste, M. S., Peñaranda, S. & Pallansch, M. A. ( 2004e; ). RNA recombination plays a major role in genomic change during circulation of coxsackie B viruses. J Virol 78, 2948–2955.[CrossRef]
    [Google Scholar]
  32. Oprisan, G., Combiescu, M., Guillot, S., Caro, V., Combiescu, A., Delpeyroux, F. & Crainic, R. ( 2002; ). Natural genetic recombination between co-circulating heterotypic enteroviruses. J Gen Virol 83, 2193–2200.
    [Google Scholar]
  33. Paananen, A., Ylipaasto, P., Rieder, E., Hovi, T., Galama, J. M. & Roivainen, M. ( 2003; ). Molecular and biological analysis of echovirus 9 strain isolated from a diabetic child. J Med Virol 69, 529–537.[CrossRef]
    [Google Scholar]
  34. Pallansch, M. A. & Roos, R. P. ( 2001; ). Enteroviruses: polioviruses, coxsackieviruses, echoviruses, and newer enteroviruses. In Fields Virology, 4th edn, pp. 723–775. Edited by D. M. Knipe, P. M. Howley, D. E. Griffin, R. A. Lamb, M. A. Martin, B. Roizman & S. E. Straus. Philadelphia: Lippincott Williams & Wilkins.
  35. Santti, J., Hyypia, T., Kinnunen, L. & Salminen, M. ( 1999; ). Evidence of recombination among enteroviruses. J Virol 73, 8741–8749.
    [Google Scholar]
  36. Schmidt, N. J., Lennette, E. H. & Ho, H. H. ( 1974; ). An apparently new enterovirus isolated from patients with disease of the central nervous system. J Infect Dis 129, 304–309.[CrossRef]
    [Google Scholar]
  37. Zimmermann, H., Eggers, H. J. & Nelsen-Salz, B. ( 1996; ). Molecular cloning and sequence determination of the complete genome of the virulent echovirus 9 strain Barty. Virus Genes 12, 149–154.[CrossRef]
    [Google Scholar]
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vol. , part 2, pp. 445 - 451

Regions sequenced and GenBank accession numbers for strains analysed and identity and GenBank accession numbers of sequences used in pairwise comparisons and phylogenetic trees. [PDF](180 KB)



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