1887

Abstract

Herpes simplex virus (HSV) capsids assemble, mature and package their viral genome in the nucleoplasm. They then exit the nucleus into the cytoplasm, where they acquire their final tegument and envelope. The molecular mechanism of cytoplasmic envelopment is unclear, but evidence suggests that the viral glycoprotein tails play an important role in the recruitment of tegument and capsids at the final envelopment site. However, due to redundancy in protein–protein interactions among the viral glycoproteins, genetic analysis of the role of individual glycoproteins in assembly has been difficult. To overcome this problem, a glutathione -transferase fusion protein-binding assay was used in this study to test the interaction between the cytoplasmic tail of one specific viral glycoprotein, gD, and tegument proteins. The study demonstrated that the 38 kDa tegument protein VP22 bound specifically to the gD tail. This association was dependent on arginine and lysine residues at positions 5 and 6 in the gD tail. In addition, HSV-1 capsids bound the gD tail and exhibited a similar sequence dependence. It is concluded that VP22 may serve as a linker protein, mediating the interaction of the HSV capsid with gD.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.80444-0
2005-02-01
2019-11-14
Loading full text...

Full text loading...

/deliver/fulltext/jgv/86/2/vir860253.html?itemId=/content/journal/jgv/10.1099/vir.0.80444-0&mimeType=html&fmt=ahah

References

  1. Baines, J. D., Cunningham, C., Nalwanga, D. & Davison, A. ( 1997; ). The UL15 gene of herpes simplex virus type 1 contains within its second exon a novel open reading frame that is translated in frame with the UL15 gene product. J Virol 71, 2666–2673.
    [Google Scholar]
  2. Brack, A. R., Dijkstra, J. M., Granow, H., Klupp, B. G. & Mettenleiter, T. C. ( 1999; ). Inhibition of virion maturation by simultaneous deletion of glycoproteins E, I and M of pseudorabies virus. J Virol 73, 5364–5372.
    [Google Scholar]
  3. Brack, A. R., Klupp, B. G., Granzow, H., Tirabassi, R., Enquist, L. W. & Mettenleiter, T. C. ( 2000; ). Role of the cytoplasmic tail of pseudorabies virus glycoprotein E in virion formation. J Virol 74, 4004–4016.[CrossRef]
    [Google Scholar]
  4. Browne, H., Bell, S. & Minson, T. ( 2004; ). Analysis of the requirement for glycoprotein M in herpes simplex virus type 1 morphogenesis. J Virol 78, 1039–1041.[CrossRef]
    [Google Scholar]
  5. Church, G. A., Dasgupta, A. & Wilson, D. W. ( 1998; ). Herpes simplex virus DNA packaging without measurable DNA synthesis. J Virol 72, 2745–2751.
    [Google Scholar]
  6. Dasgupta, A. & Wilson, D. W. ( 1999; ). ATP depletion blocks herpes simplex virus DNA packaging and capsid maturation. J Virol 73, 2006–2015.
    [Google Scholar]
  7. Elliott, G., Mouzakitis, G. & O'Hare, P. ( 1995; ). VP16 interacts via its activation domain with VP22, a tegument protein of herpes simplex virus, and is relocated to a novel macromolecular assembly in coexpressing cells. J Virol 69, 7932–7941.
    [Google Scholar]
  8. Farnsworth, A., Goldsmith, K. & Johnson, D. C. ( 2003; ). Herpes simplex virus glycoproteins gD and gE/gI serve essential but redundant functions during acquisition of the virion envelope in the cytoplasm. J Virol 77, 8481–8494.[CrossRef]
    [Google Scholar]
  9. Feenstra, V., Hodaie, M. & Johnson, D. C. ( 1990; ). Deletions in herpes simplex virus glycoprotein D define nonessential and essential domains. J Virol 64, 2096–2102.
    [Google Scholar]
  10. Fuchs, W., Klupp, B. G., Granzow, H., Hengartner, C., Brack, A., Mundt, A., Enquist, L. W. & Mettenleiter, T. C. ( 2002; ). Physical interaction between envelope glycoproteins E and M of pseudorabies virus and the major tegument protein UL49. J Virol 76, 8208–8217.[CrossRef]
    [Google Scholar]
  11. Gross, S. T., Harley, C. A. & Wilson, D. W. ( 2003; ). The cytoplasmic tail of herpes simplex virus glycoprotein H binds to the tegument protein VP16 in vitro and in vivo. Virology 317, 1–12.[CrossRef]
    [Google Scholar]
  12. Guan, K. L. & Dixon, J. E. ( 1991; ). Eukaryotic proteins expressed in Escherichia coli: an improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase. Anal Biochem 192, 262–267.[CrossRef]
    [Google Scholar]
  13. Harley, C. A., Dasgupta, A. & Wilson, D. W. ( 2001; ). Characterization of herpes simplex virus-containing organelles by subcellular fractionation: role for organelle acidification in assembly of infectious particles. J Virol 75, 1236–1251.[CrossRef]
    [Google Scholar]
  14. Jones, F. E., Smibert, C. A. & Smiley, J. R. ( 1995; ). Mutational analysis of the herpes simplex virus virion host shutoff protein: evidence that vhs functions in the absence of other viral proteins. J Virol 69, 4863–4871.
    [Google Scholar]
  15. Mettenleiter, T. C. ( 2002; ). Herpesvirus assembly and egress. J Virol 76, 1537–1547.[CrossRef]
    [Google Scholar]
  16. Newcomb, W. W., Homa, F. L., Thomsen, D. R., Booy, F. P., Trus, B. L., Steven, A. C., Spencer, J. V. & Brown, J. C. ( 1996; ). Assembly of the herpes simplex virus capsid: characterization of intermediates observed during cell-free capsid formation. J Mol Biol 263, 432–446.[CrossRef]
    [Google Scholar]
  17. Pomeranz, L. E. & Blaho, J. A. ( 1999; ). Modified VP22 localizes to the cell nucleus during synchronized herpes simplex virus type 1 infection. J Virol 73, 6769–6781.
    [Google Scholar]
  18. Roizman, B. & Pellett, P. E. ( 2001; ). The family Herpesviridae: a brief introduction. In Field's Virology, 4th edn, vol. 2, pp. 2381–2397. Edited by D. M. Knipe & P. M. Howley. Philadelphia: Lippencott Williams & Wilkins.
  19. Spear, P. G. & Roizman, B. ( 1972; ). Proteins specified by herpes simplex virus. V. Purification and structural proteins of the herpesvirion. J Virol 9, 143–159.
    [Google Scholar]
  20. Trus, B. L., Booy, F. P., Newcomb, W. W., Brown, J. C., Homa, F. L., Thomsen, D. R. & Steven, A. C. ( 1996; ). The herpes simplex virus procapsid: structure, conformational changes upon maturation, and roles of the triplex proteins VP19c and VP23 in assembly. J Mol Biol 263, 447–462.[CrossRef]
    [Google Scholar]
  21. Zhu, Q. & Courtney, R. J. ( 1994; ). Chemical cross-linking of virion envelope and tegument proteins of herpes simplex virus type 1. Virology 204, 590–599.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.80444-0
Loading
/content/journal/jgv/10.1099/vir.0.80444-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error