Non-pathogenic porcine circovirus type 1 (PCV1) and pathogenic PCV2 are widespread in swine herds. In this study, the detection and characterization of PCV1 and PCV2 DNA from porcine-derived commercial pepsin are reported. The complete genomic sequences of the pepsin-derived PCV1 and PCV2 share 76 % nucleotide sequence identity with each other and 95–99 % identity with respective North American PCV1 and PCV2 isolates. However, the PCV-contaminated pepsin lacks infectivity in PK-15 cells. To further assess the infectivity of the contaminating pepsin in vivo, 16 5-week-old, specific-pathogen-free pigs were divided randomly into three groups: pigs in group 1 (n=5) were each inoculated intramuscularly and intranasally with 4 ml PBS buffer as negative controls, those in group 2 (n=6) were each inoculated with 400 mg contaminated pepsin dissolved in 4 ml PBS and those in group 3 (n=5) were each inoculated with 4×104·3 TCID50 PCV2 as positive controls. PCV2 viraemia, seroconversion and pathological lesions were detected in group 3 pigs, but not in group 1 or 2 pigs, confirming that the contaminating PCVs were non-infectious. Nevertheless, the detection of PCV DNA in a porcine-derived commercial product raises concern for potential human infection through xenotransplantation.
AllanG. M.,
McNeillyF.,
CassidyJ. P.,
ReillyG. A.,
AdairB.,
EllisW. A. C.,
McNultyM. S.1995; Pathogenesis of porcine circovirus; experimental infections of colostrum deprived piglets and examination of pig foetal material. Vet Microbiol 44:49–64[CrossRef]
AllanG. M.,
McNeillyF.,
KennedyS.,
DaftB.,
ClarkE. G.,
EllisJ. A.,
HainesD. M.,
MeehanB. M.,
AdairB. M.1998; Isolation of porcine circovirus-like viruses from pigs with a wasting disease in the USA and Europe. J Vet Diagn Invest 10:3–10[CrossRef]
EllisJ. A.,
WisemanB. M.,
AllanG.,
KonobyC.,
KrakowkaS.,
MeehanB. M.,
McNeillyF.2000; Analysis of seroconversion to porcine circovirus 2 among veterinarians from the United States and Canada. J Am Vet Med Assoc 217:1645–1646[CrossRef]
FenauxM.,
HalburP. G.,
GillM.,
TothT. E.,
MengX.-J.2000; Genetic characterization of type 2 porcine circovirus (PCV-2) from pigs with postweaning multisystemic wasting syndrome in different geographic regions of North America and development of a differential PCR-restriction fragment length polymorphism assay to detect and differentiate between infections with PCV-1 and PCV-2. J Clin Microbiol 38:2494–2503
FenauxM.,
HalburP. G.,
HaqshenasG.,
RoyerR.,
ThomasP.,
NawagitgulP.,
GillM.,
TothT. E.,
MengX. J.2002; Cloned genomic DNA of type 2 porcine circovirus is infectious when injected directly into the liver and lymph nodes of pigs: characterization of clinical disease, virus distribution, and pathologic lesions. J Virol 76:541–551[CrossRef]
FenauxM.,
OpriessnigT.,
HalburP. G.,
MengX. J.2003; Immunogenicity and pathogenicity of chimeric infectious DNA clones of pathogenic porcine circovirus type 2 (PCV2) and nonpathogenic PCV1 in weanling pigs. J Virol 77:11232–11243[CrossRef]
FenauxM.,
OpriessnigT.,
HalburP. G.,
ElvingerF.,
MengX. J.2004a; A chimeric porcine circovirus (PCV) with the immunogenic capsid gene of the pathogenic PCV type 2 (PCV2) cloned into the genomic backbone of the nonpathogenic PCV1 induces protective immunity against PCV2 infection in pigs. J Virol 78:6297–6303[CrossRef]
FenauxM.,
OpriessnigT.,
HalburP. G.,
ElvingerF.,
MengX. J.2004b; Two amino acid mutations in the capsid protein of type 2 porcine circovirus (PCV2) enhanced PCV2 replication in vitro and attenuated the virus in vivo
. J Virol (in press
HalburP. G.,
PaulP. S.,
FreyM. L.,
LandgrafJ.,
EernisseK.,
MengX. J.,
LumM. A.,
AndrewsJ. J.,
RathjeJ. A.1995; Comparison of the pathogenicity of two US porcine reproductive and respiratory syndrome virus isolates with that of the Lelystad virus. Vet Pathol 32:648–660[CrossRef]
HarmsP. A.,
SordenS. D.,
HalburP. G.,
BolinS. R.,
LagerK. M.,
MorozovI.,
PaulP. S.2001; Experimental reproduction of severe disease in CD/CD pigs concurrently infected with type 2 porcine circovirus and porcine reproductive and respiratory syndrome virus. Vet Pathol 38:528–539[CrossRef]
KiupelM.,
StevensonG. W.,
ChoiJ.,
LatimerK. S.,
KanitzC. L.,
MittalS. K.2001; Viral replication and lesions in BALB/c mice experimentally inoculated with porcine circovirus isolated from a pig with postweaning multisystemic wasting disease. Vet Pathol 38:74–82[CrossRef]
LekcharoensukP.,
MorozovI.,
PaulP. S.,
ThangthumniyomN.,
WajjawalkuW.,
MengX. J.2004; Epitope mapping of the major capsid protein of type 2 porcine circovirus (PCV2) by using chimeric PCV1 and PCV2. J Virol 78:8135–8145[CrossRef]
NawagitgulP.,
HarmsP. A.,
MorozovI.,
ThackerB. J.,
SordenS. D.,
LekcharoensukC.,
PaulP. S.2002; Modified indirect porcine circovirus (PCV) type 2-based and recombinant capsid protein (ORF2)-based enzyme-linked immunosorbent assays for detection of antibodies to PCV. Clin Diagn Lab Immunol 9:33–40
QuintanaJ.,
BalaschM.,
SegalésJ.,
CalsamigliaM.,
Rodríguez-ArriojaG. M.,
Plana-DuránJ.,
DomingoM.2002; Experimental inoculation of porcine circoviruses type 1 (PCV1) and type 2 (PCV2) in rabbits and mice. Vet Res 33:229–237[CrossRef]
RocaM.,
BalaschM.,
SegalésJ.7 other authors2004; In vitro and in vivo characterization of an infectious clone of a European strain of porcine circovirus type 2. J Gen Virol 85:1259–1266[CrossRef]
RoyerR. L.,
NawagitgulP.,
HalburP. G.,
PaulP. S.2001; Susceptibility of porcine circovirus type 2 to commercial and laboratory disinfectants. J Swine Health Prod 9:281–284
SordenS. D.,
HarmsP. A.,
NawagitgulP.,
CavanaughD.,
PaulP. S.1999; Development of a polyclonal-antibody-based immunohistochemical method for the detection of type 2 porcine circovirus in formalin-fixed, paraffin-embedded tissue. J Vet Diagn Invest 11:528–530[CrossRef]
SoucieJ. M.,
ErdmanD. D.,
EvattB. L. 8 other authors; 2000; Investigation of porcine parvovirus among persons with hemophilia receiving Hyate : C porcine factor VIII concentrate. Transfusion 40:708–711[CrossRef]
TakefmanD. M.,
WongS.,
MaudruT.,
PedenK.,
WilsonC. A.2001; Detection and characterization of porcine endogenous retrovirus in porcine plasma and porcine factor VIII. J Virol 75:4551–4557[CrossRef]
TuckerA. W.,
McNeillyF.,
MeehanB.,
GalbraithD.,
McArdleP. D.,
AllanG.,
PatienceC.2003; Methods for the exclusion of circoviruses and gammaherpesviruses from pigs. Xenotransplantation 10:343–348[CrossRef]