1887

Abstract

Non-pathogenic porcine circovirus type 1 (PCV1) and pathogenic PCV2 are widespread in swine herds. In this study, the detection and characterization of PCV1 and PCV2 DNA from porcine-derived commercial pepsin are reported. The complete genomic sequences of the pepsin-derived PCV1 and PCV2 share 76 % nucleotide sequence identity with each other and 95–99 % identity with respective North American PCV1 and PCV2 isolates. However, the PCV-contaminated pepsin lacks infectivity in PK-15 cells. To further assess the infectivity of the contaminating pepsin , 16 5-week-old, specific-pathogen-free pigs were divided randomly into three groups: pigs in group 1 (=5) were each inoculated intramuscularly and intranasally with 4 ml PBS buffer as negative controls, those in group 2 (=6) were each inoculated with 400 mg contaminated pepsin dissolved in 4 ml PBS and those in group 3 (=5) were each inoculated with 4×10 TCID PCV2 as positive controls. PCV2 viraemia, seroconversion and pathological lesions were detected in group 3 pigs, but not in group 1 or 2 pigs, confirming that the contaminating PCVs were non-infectious. Nevertheless, the detection of PCV DNA in a porcine-derived commercial product raises concern for potential human infection through xenotransplantation.

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2004-11-01
2019-10-18
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References

  1. Allan, G. M. & Ellis, J. A. ( 2000; ). Porcine circoviruses: a review. J Vet Diagn Invest 12, 3–14.[CrossRef]
    [Google Scholar]
  2. Allan, G. M., McNeilly, F., Cassidy, J. P., Reilly, G. A., Adair, B., Ellis, W. A. C. & McNulty, M. S. ( 1995; ). Pathogenesis of porcine circovirus; experimental infections of colostrum deprived piglets and examination of pig foetal material. Vet Microbiol 44, 49–64.[CrossRef]
    [Google Scholar]
  3. Allan, G. M., McNeilly, F., Kennedy, S., Daft, B., Clark, E. G., Ellis, J. A., Haines, D. M., Meehan, B. M. & Adair, B. M. ( 1998; ). Isolation of porcine circovirus-like viruses from pigs with a wasting disease in the USA and Europe. J Vet Diagn Invest 10, 3–10.[CrossRef]
    [Google Scholar]
  4. Ellis, J. A., Wiseman, B. M., Allan, G., Konoby, C., Krakowka, S., Meehan, B. M. & McNeilly, F. ( 2000; ). Analysis of seroconversion to porcine circovirus 2 among veterinarians from the United States and Canada. J Am Vet Med Assoc 217, 1645–1646.[CrossRef]
    [Google Scholar]
  5. Fenaux, M., Halbur, P. G., Gill, M., Toth, T. E. & Meng, X.-J. ( 2000; ). Genetic characterization of type 2 porcine circovirus (PCV-2) from pigs with postweaning multisystemic wasting syndrome in different geographic regions of North America and development of a differential PCR-restriction fragment length polymorphism assay to detect and differentiate between infections with PCV-1 and PCV-2. J Clin Microbiol 38, 2494–2503.
    [Google Scholar]
  6. Fenaux, M., Halbur, P. G., Haqshenas, G., Royer, R., Thomas, P., Nawagitgul, P., Gill, M., Toth, T. E. & Meng, X. J. ( 2002; ). Cloned genomic DNA of type 2 porcine circovirus is infectious when injected directly into the liver and lymph nodes of pigs: characterization of clinical disease, virus distribution, and pathologic lesions. J Virol 76, 541–551.[CrossRef]
    [Google Scholar]
  7. Fenaux, M., Opriessnig, T., Halbur, P. G. & Meng, X. J. ( 2003; ). Immunogenicity and pathogenicity of chimeric infectious DNA clones of pathogenic porcine circovirus type 2 (PCV2) and nonpathogenic PCV1 in weanling pigs. J Virol 77, 11232–11243.[CrossRef]
    [Google Scholar]
  8. Fenaux, M., Opriessnig, T., Halbur, P. G., Elvinger, F. & Meng, X. J. ( 2004a; ). A chimeric porcine circovirus (PCV) with the immunogenic capsid gene of the pathogenic PCV type 2 (PCV2) cloned into the genomic backbone of the nonpathogenic PCV1 induces protective immunity against PCV2 infection in pigs. J Virol 78, 6297–6303.[CrossRef]
    [Google Scholar]
  9. Fenaux, M., Opriessnig, T., Halbur, P. G., Elvinger, F. & Meng, X. J. ( 2004b; ). Two amino acid mutations in the capsid protein of type 2 porcine circovirus (PCV2) enhanced PCV2 replication in vitro and attenuated the virus in vivo. J Virol (in press).
    [Google Scholar]
  10. Halbur, P. G., Paul, P. S., Frey, M. L., Landgraf, J., Eernisse, K., Meng, X. J., Lum, M. A., Andrews, J. J. & Rathje, J. A. ( 1995; ). Comparison of the pathogenicity of two US porcine reproductive and respiratory syndrome virus isolates with that of the Lelystad virus. Vet Pathol 32, 648–660.[CrossRef]
    [Google Scholar]
  11. Harding, J. C. & Clark, E. G. ( 1997; ). Recognizing and diagnosing post-weaning multisystemic wasting syndrome (PMWS). J Swine Health Prod 5, 201–203.
    [Google Scholar]
  12. Harms, P. A., Sorden, S. D., Halbur, P. G., Bolin, S. R., Lager, K. M., Morozov, I. & Paul, P. S. ( 2001; ). Experimental reproduction of severe disease in CD/CD pigs concurrently infected with type 2 porcine circovirus and porcine reproductive and respiratory syndrome virus. Vet Pathol 38, 528–539.[CrossRef]
    [Google Scholar]
  13. Kiupel, M., Stevenson, G. W., Choi, J., Latimer, K. S., Kanitz, C. L. & Mittal, S. K. ( 2001; ). Viral replication and lesions in BALB/c mice experimentally inoculated with porcine circovirus isolated from a pig with postweaning multisystemic wasting disease. Vet Pathol 38, 74–82.[CrossRef]
    [Google Scholar]
  14. Ladekjær-Mikkelsen, A. S., Nielsen, J., Stadejek, T., Storgaard, T., Krakowka, S., Ellis, J., McNeilly, F., Allan, G. & Bøtner, A. ( 2002; ). Reproduction of postweaning multisystemic wasting syndrome (PMWS) in immunostimulated and non-immunostimulated 3-week-old piglets experimentally infected with porcine circovirus type 2 (PCV2). Vet Microbiol 89, 97–114.[CrossRef]
    [Google Scholar]
  15. Lekcharoensuk, P., Morozov, I., Paul, P. S., Thangthumniyom, N., Wajjawalku, W. & Meng, X. J. ( 2004; ). Epitope mapping of the major capsid protein of type 2 porcine circovirus (PCV2) by using chimeric PCV1 and PCV2. J Virol 78, 8135–8145.[CrossRef]
    [Google Scholar]
  16. Mankertz, A., Çaliskan, R., Hattermann, K., Hillenbrand, B., Kurzendoerfer, P., Mueller, B., Schmitt, C., Steinfeldt, T. & Finsterbusch, T. ( 2004; ). Molecular biology of Porcine circovirus: analyses of gene expression and viral replication. Vet Microbiol 98, 81–88.[CrossRef]
    [Google Scholar]
  17. Meng, X. J. ( 2003; ). Swine hepatitis E virus: cross-species infection and risk in xenotransplantation. Curr Top Microbiol Immunol 278, 185–216.
    [Google Scholar]
  18. Nawagitgul, P., Harms, P. A., Morozov, I., Thacker, B. J., Sorden, S. D., Lekcharoensuk, C. & Paul, P. S. ( 2002; ). Modified indirect porcine circovirus (PCV) type 2-based and recombinant capsid protein (ORF2)-based enzyme-linked immunosorbent assays for detection of antibodies to PCV. Clin Diagn Lab Immunol 9, 33–40.
    [Google Scholar]
  19. Quintana, J., Balasch, M., Segalés, J., Calsamiglia, M., Rodríguez-Arrioja, G. M., Plana-Durán, J. & Domingo, M. ( 2002; ). Experimental inoculation of porcine circoviruses type 1 (PCV1) and type 2 (PCV2) in rabbits and mice. Vet Res 33, 229–237.[CrossRef]
    [Google Scholar]
  20. Roca, M., Balasch, M., Segalés, J. & 7 other authors ( 2004; ). In vitro and in vivo characterization of an infectious clone of a European strain of porcine circovirus type 2. J Gen Virol 85, 1259–1266.[CrossRef]
    [Google Scholar]
  21. Rovira, A., Balasch, M., Segalés, J., García, L., Plana-Durán, J., Rosell, C., Ellerbrok, H., Mankertz, A. & Domingo, M. ( 2002; ). Experimental inoculation of conventional pigs with porcine reproductive and respiratory syndrome virus and porcine circovirus 2. J Virol 76, 3232–3239.[CrossRef]
    [Google Scholar]
  22. Royer, R. L., Nawagitgul, P., Halbur, P. G. & Paul, P. S. ( 2001; ). Susceptibility of porcine circovirus type 2 to commercial and laboratory disinfectants. J Swine Health Prod 9, 281–284.
    [Google Scholar]
  23. Sorden, S. D., Harms, P. A., Nawagitgul, P., Cavanaugh, D. & Paul, P. S. ( 1999; ). Development of a polyclonal-antibody-based immunohistochemical method for the detection of type 2 porcine circovirus in formalin-fixed, paraffin-embedded tissue. J Vet Diagn Invest 11, 528–530.[CrossRef]
    [Google Scholar]
  24. Soucie, J. M., Erdman, D. D., Evatt, B. L. & 8 other authors ( 2000; ). Investigation of porcine parvovirus among persons with hemophilia receiving Hyate : C porcine factor VIII concentrate. Transfusion 40, 708–711.[CrossRef]
    [Google Scholar]
  25. Takefman, D. M., Wong, S., Maudru, T., Peden, K. & Wilson, C. A. ( 2001; ). Detection and characterization of porcine endogenous retrovirus in porcine plasma and porcine factor VIII. J Virol 75, 4551–4557.[CrossRef]
    [Google Scholar]
  26. Tischer, I., Gelderblom, H., Vettermann, W. & Koch, M. A. ( 1982; ). A very small porcine virus with circular single-stranded DNA. Nature 295, 64–66.[CrossRef]
    [Google Scholar]
  27. Tischer, I., Mields, W., Wolff, D., Vagt, M. & Griem, W. ( 1986; ). Studies on epidemiology and pathogenicity of porcine circovirus. Arch Virol 91, 271–276.[CrossRef]
    [Google Scholar]
  28. Tucker, A. W., McNeilly, F., Meehan, B., Galbraith, D., McArdle, P. D., Allan, G. & Patience, C. ( 2003; ). Methods for the exclusion of circoviruses and gammaherpesviruses from pigs. Xenotransplantation 10, 343–348.[CrossRef]
    [Google Scholar]
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