RT Journal Article SR Electronic(1) A1 Puthavathana, Pilaipan A1 Auewarakul, Prasert A1 Charoenying, Pakapak Chor A1 Sangsiriwut, Kantima A1 Pooruk, Phisanu A1 Boonnak, Kobporn A1 Khanyok, Raweewan A1 Thawachsupa, Pranee A1 Kijphati, Rungrueng A1 Sawanpanyalert, PathomYR 2005 T1 Molecular characterization of the complete genome of human influenza H5N1 virus isolates from Thailand JF Journal of General Virology, VO 86 IS 2 SP 423 OP 433 DO https://doi.org/10.1099/vir.0.80368-0 PB Microbiology Society, SN 1465-2099, AB The complete genomes of three human H5N1 influenza isolates were characterized, together with the haemagglutinin (HA) and neuraminidase (NA) genes from two additional human isolates and one chicken isolate. These six influenza isolates were obtained from four different provinces of Thailand during the avian influenza outbreak in Asia from late 2003 to May 2004. All six Thailand isolates contained multiple basic amino acids at the cleavage site in the HA gene. Amino acid residues at the receptor-binding site of the five human viruses were similar to those of the chicken virus and other H5N1 viruses from Hong Kong. The presence of amantadine resistance in the Thailand viruses isolated during this outbreak was suggested by a fixed mutation in M2 and confirmed by a phenotypic assay. All genomic segments of the Thailand viruses clustered with the recently described genotype Z. The Thailand viruses contained more avian-specific residues than the 1997 Hong Kong H5N1 viruses, suggesting that the virus may have adapted to allow a more efficient spread in avian species., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.80368-0