Ovine pulmonary adenocarcinoma (OPA) can be reproduced consistently in neonatal lambs by intratracheal injection of inocula containing jaagsiekte sheep retrovirus (JSRV). In this study, clinical disease, confirmed pathologically as OPA, was induced in a high proportion of lambs that had been inoculated intratracheally with infectious lung fluid at 1, 3 and 6 months of age. The incubation periods, however, were longer in these three age groups than in 1-week-old lambs that were used as controls. Viraemia was detected in all age groups before onset of clinical signs, but occurred later in older animals. These results suggest an age-dependent susceptibility to OPA that could be determined by the availability of JSRV target cells in the ovine lung. The feasibility of inducing OPA in older lambs and detecting JSRV viraemia in preclinical stages enables improved studies on the pathogenesis, assessment of vaccines, diagnosis and control of the disease.
AndressonÓ. S.,
ElserJ. E.,
TobinG. J.11 other authors1993; Nucleotide sequence and biological properties of a pathogenic proviral molecular clone of neurovirulent visna virus. Virology 193:89–105[CrossRef]
DeMartiniJ. C.,
RosadioR. H.,
SharpJ. M.,
RussellH. I.,
LairmoreM. D.1987; Experimental coinduction of type D retrovirus-associated pulmonary carcinoma and lentivirus-associated lymphoid interstitial pneumonia in lambs. J Natl Cancer Inst 79:167–177
DeMartiniJ. C.,
BishopJ. V.,
AllenT. E.,
JassimF. A.,
SharpJ. M.,
de las HerasM.,
VoelkerD. R.,
CarlsonJ. O.2001; Jaagsiekte sheep retrovirus proviral clone JSRVJS7, derived from the JS7 lung tumor cell line, induces ovine pulmonary carcinoma and is integrated into the surfactant protein A gene. J Virol 75:4239–4246[CrossRef]
García-GotiM.1999; Estudios sobre la patogenia y diagnóstico de la adenomatosis pulmonary ovina
. PhD thesis University of Zaragoza; Spain: (in Spanish)
GonzálezL.,
García-GotiM.,
CousensC.,
DewarP.,
CortabarríaN.,
ExtramianaA. B.,
OrtínA.,
De las HerasM.,
SharpJ. M.2001; Jaagsiekte sheep retrovirus can be detected in the peripheral blood during the pre-clinical period of sheep pulmonary adenomatosis. J Gen Virol 82:1355–1358
HerringA. J.,
SharpJ. M.,
ScottF. M. M.,
AngusK. W.1983; Further evidence for a retrovirus as the aetiological agent of sheep pulmonary adenomatosis (jaagsiekte). Vet Microbiol 8:237–249[CrossRef]
JassimF. A.,
SharpJ. M.,
MarinelloP. D.1987; Three-step procedure for isolation of epithelial cells from the lungs of sheep with jaagsiekte. Res Vet Sci 43:407–409
KahwaC. K.,
AtwalO. S.,
PurtonM.1997; Transmission electron microscopy of the epithelium of distal airways and pulmonary parenchyma of the goat lung. Res Vet Sci 63:49–56[CrossRef]
KahwaC. K.,
BalembaO. B.,
AsseyR. J.2000; The pattern of ciliation and the development of the epithelial lining of the respiratory tract in the neonatal kid: a scanning electron microscopic study. Small Rumin Res 37:27–34[CrossRef]
OrtínA.,
MinguijonE.,
DewarP.,
GarciaM.,
FerrerL. M.,
PalmariniM.,
GonzálezL.,
SharpJ. M.,
De las HerasM.1998; Lack of a specific immune response against a recombinant capsid protein of Jaagsiekte sheep retrovirus in sheep and goats naturally affected by enzootic nasal tumour or sheep pulmonary adenomatosis. Vet Immunol Immunopathol 61:229–237[CrossRef]
PalmariniM.,
DewarP.,
De las HerasM.,
InglisN. F.,
DalzielR. G.,
SharpJ. M.1995; Epithelial tumour cells in the lungs of sheep with pulmonary adenomatosis are major sites of replication for Jaagsiekte retrovirus. J Gen Virol 76:2731–2737[CrossRef]
PalmariniM.,
HollandM. J.,
CousensC.,
DalzielR. G.,
SharpJ. M.1996; Jaagsiekte retrovirus establishes a disseminated infection of the lymphoid tissues of sheep affected by pulmonary adenomatosis. J Gen Virol 77:2991–2998[CrossRef]
PalmariniM.,
MuraM.,
SpencerT. E.2004; Endogenous betaretroviruses of sheep: teaching new lessons in retroviral interference and adaptation. J Gen Virol 85:1–13[CrossRef]
PizerE. S.,
BabaT. W.,
HumphriesE. H.1992; Activation of the c- myb locus is insufficient for the rapid induction of disseminated avian B-cell lymphoma. J Virol 66:512–523
PlattJ. A.,
KraipowichN.,
VillafaneF.,
DeMartiniJ. C.2002; Alveolar type II cells expressing jaagsiekte sheep retrovirus capsid protein and surfactant proteins are the predominant neoplastic cell type in ovine pulmonary adenocarcinoma. Vet Pathol 39:341–352[CrossRef]
RosadioR. H.,
LairmoreM. D.,
RussellH. I.,
DeMartiniJ. C.1988; Retrovirus-associated ovine pulmonary carcinoma (sheep pulmonary adenomatosis) and lymphoid interstitial pneumonia. I. Lesion development and age susceptibility. Vet Pathol 25:475–483[CrossRef]
SharpJ. M.,
HerringA. J.1983; Sheep pulmonary adenomatosis: demonstration of a protein which cross-reacts with the major core proteins of Mason–Pfizer monkey virus and mouse mammary tumour virus. J Gen Virol 64:2323–2327[CrossRef]
SharpJ. M.,
AngusK. W.,
GrayE. W.,
ScottF. M.1983; Rapid transmission of sheep pulmonary adenomatosis (jaagsiekte) in young lambs. Arch Virol 78:89–95[CrossRef]
StedmanN. L.,
BrownT. P.,
BrownC. C.2001; Localization of avian leukosis virus subgroup J in naturally infected chickens by RNA in situ hybridization. Vet Pathol 38:649–656[CrossRef]
VerwoerdD. W.,
WilliamsonA.-L.,
De VilliersE.-M.1980; Aetiology of jaagsiekte: transmission by means of subcellular fractions and evidence for the involvement of a retrovirus. Onderstepoort J Vet Res 47:275–280
WeibelE. R.,
TaylorC. R.1996; Functional anatomy of the human lung. In Oxford Textbook of Medicine, 3rd edn. pp 2593–2609 Edited by
WeatherallD. J.,
LedinghamJ. G. G.,
WarrellD. A.
Oxford: Oxford University Press;
WrightN. A.,
AlisonM. A.1984; Cell proliferation in respiratory epithelia. In The Biology of Epithelial Cell Populationsvol. 2 pp 1068–1078 Oxford: Oxford University Press;
YorkD. F.,
VigneR.,
VerwoerdD. W.,
QueratG.1992; Nucleotide sequence of the jaagsiekte retrovirus, an exogenous and endogenous type D and B retrovirus of sheep and goats. J Virol 66:4930–4939