1887

Abstract

Natural recombinant (PPV) isolates were detected in Albania, Bulgaria, Czech Republic, Germany, Hungary and Slovakia. Despite different geographical origins and dates of isolation, all the recombinant isolates were closely related at the molecular level and shared the same recombination breakpoint as well as a typical signature in their N-terminal coat protein sequence, suggesting a common origin. Biological assays with four recombinant isolates demonstrated their capacity to be aphid-transmitted to various hosts. One of these isolates had a threonine-to-isoleucine mutation in the conserved PTK motif of its and showed a drastically decreased, although not abolished, aphid transmissibility. The complete genome sequence of one of the recombinant isolates, BOR-3, was determined, as well as some partial sequences in the and genes for additional natural recombinant isolates. Analysis of the phylogenetic relationships between the recombinant isolates and other sequenced PPV isolates confirmed that the recombinant isolates form a phylogenetically homogeneous lineage. In addition, this analysis revealed an ancient recombination event between the PPV-D and M subgroups, with a recombination breakpoint located in the gene. Taken together, these results indicate that recombinant isolates represent an evolutionarily successful, homogeneous group of isolates with a common history and unique founding recombination event. The name PPV-Rec is proposed for this coherent ensemble of isolates.

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2004-09-01
2020-01-26
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vol. , part 9, pp. 2671 - 2681

Multiple alignment of nucleotide sequences of isolates belonging to the PPV-D (Dideron, SC, PENN-2) and PPV-M (PS, SK68) subgroups and of recombinant isolates (Rec). Only the region around the C terminus of the gene containing the recombination crossover is shown (positions 8341-8520 numbered according to the genomic sequence of BOR-3, AY028309). The position of the crossover is boxed and the numbering of the borders indicated above the alignment. Dots indicate nucleotides identical to the Dideron sequence.

Primers used to amplify the seven overlapping PCR fragments covering the whole BOR-3 genome.

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