1887

Abstract

In this study, quantitative parameters affecting acquisition and whitefly () transmission of (LIYV) were examined and transmission of an engineered defective RNA (D-RNA) was demonstrated. Virions purified from virus- and virion RNA-inoculated plants and protoplasts of , respectively, were consistently transmitted to plants by when virion concentrations were 0·1 ng μl or greater. Transmission efficiency increased with increasing virion concentration and number of whiteflies used for inoculation. When -derived transcripts of the M5gfp D-RNA (engineered to express the green fluorescent protein, GFP) were co-inoculated to protoplasts with wild-type LIYV virion RNAs, the resulting virions were transmissible to plants. LIYV and the M5gfp D-RNA systemically invaded inoculated plants; however, GFP expression was not detected in these plants. Unlike LIYV, the M5gfp D-RNA was not subsequently transmitted by from the initially infected plants, but, when high concentrations of virions from plants infected by LIYV and the M5gfp D-RNA were used for acquisition by whiteflies, both were transmitted to plants. Quantitative and qualitative analyses showed that, although the M5gfp D-RNA replicated within and systemically invaded plants along with LIYV, compared with LIYV RNA 2 it was not as abundant in plants or in the resulting virions, and concentration of encapsidated RNAs is an important factor affecting transmission efficiency.

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2004-09-01
2024-03-28
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References

  1. Agranovsky A. A., Lesemann D. E., Maiss E., Hull R., Atabekov J. G. 1995; ‘Rattlesnake’ structure of a filamentous plant RNA virus built of two capsid proteins. Proc Natl Acad Sci U S A 92:2470–2473 [CrossRef]
    [Google Scholar]
  2. Albiach-Marti M. R., Guerri J., de Mendoza A. H., Laigret F., Ballester-Olmos J. F., Moreno P. 2000; Aphid transmission alters the genomic and defective RNA populations of Citrus tristeza virus isolates. Phytopathology 90:134–138 [CrossRef]
    [Google Scholar]
  3. Atreya C. D., Pirone T. P. 1993; Mutational analysis of the helper component-proteinase gene of a potyvirus: effects of amino acid substitutions, deletions, and gene replacement on virulence and aphid transmissibility. Proc Natl Acad Sci U S A 90:11919–11923 [CrossRef]
    [Google Scholar]
  4. Atreya C. D., Atreya P. L., Thornbury D. W., Pirone T. P. 1992; Site-directed mutations in the potyvirus HC-Pro gene affect helper component activity, virus accumulation, and symptom expression in infected tobacco plants. Virology 191:106–111 [CrossRef]
    [Google Scholar]
  5. Ayllon M. A., Lopez C., Navas-Castillo J., Mawassi M., Dawson W. O., Guerri J., Flores R., Moreno P. 1999; New defective RNAs from citrus tristeza virus: evidence for a replicase-driven template switching mechanism in their generation. J Gen Virol 80:817–821
    [Google Scholar]
  6. Blanc S., Cerutti M., Usmany M., Vlak J. M., Hull R. 1993; Biological activity of cauliflower mosaic virus aphid transmission factor expressed in a heterologous system. Virology 192:643–650 [CrossRef]
    [Google Scholar]
  7. Brault V., Van Den Heuvel J. F. J. M., Verbeek M. 7 other authors 1995; Aphid transmission of beet western yellows luteovirus requires the minor capsid read-through protein P74. EMBO J 14:650–659
    [Google Scholar]
  8. Brault V., Mutterer J., Scheidecker D., Simonis M. T., Herrbach E., Richards K., Ziegler-Graff V. 2000; Effects of point mutations in the readthrough domain of the beet western yellows virus minor capsid protein on virus accumulation in planta and on transmission by aphids. J Virol 74:1140–1148 [CrossRef]
    [Google Scholar]
  9. Bruyere A., Brault V., Zieglergraff V., Simonis M. T., Vandenheuvel J., Richards K., Guilley H., Jonard G., Herrbach E. 1997; Effects of mutations in the beet western yellows virus readthrough protein on its expression and packaging and on virus accumulation, symptoms, and aphid transmission. Virology 230:323–334 [CrossRef]
    [Google Scholar]
  10. Campbell R. N. 1996; Fungal transmission of plant viruses. Annu Rev Phytopathol 34:87–108 [CrossRef]
    [Google Scholar]
  11. Chay C. A., Gunasinge U. B., Dinesh-Kumar S. P., Miller W. A., Gray S. M. 1996; Aphid transmission and systemic plant infection determinants of barley yellow dwarf luteovirus-PAV are contained in the coat protein readthrough domain and 17-kDa protein, respectively. Virology 219:57–65 [CrossRef]
    [Google Scholar]
  12. Dolja V. 2003; Beet yellows virus: the importance of being different. Mol Plant Pathol 4:91–98 [CrossRef]
    [Google Scholar]
  13. Duffus J., Larsen R., Liu H. 1986; Lettuce infectious yellows virus – a new type of whitefly-transmitted virus. Phytopathology 76:97–100 [CrossRef]
    [Google Scholar]
  14. Febres V. J., Ashoulin L., Mawassi M., Frank A., Bar-Joseph M., Manjunath K. L., Lee R. F., Niblett C. L. 1996; The p27 protein is present at one end of citrus tristeza virus particles. Phytopathology 86:1331–1335
    [Google Scholar]
  15. Gibbs A. J., Gower J. C. 1960; The use of a multiple-transfer method in plant virus transmission studies – some statistical points arising in the analysis of results. Ann Appl Biol 48:75–83 [CrossRef]
    [Google Scholar]
  16. Gildow F. E. 1999; Luteoviruses transmission and mechanisms regulating vector specificity. In The Luteoviruses pp  88–113 Edited by Smith H. G., Barker H. Wallingford, UK: CAB International;
    [Google Scholar]
  17. Gray S., Gildow F. E. 2003; Luteovirus–aphid interactions. Annu Rev Phytopathol 41:539–566 [CrossRef]
    [Google Scholar]
  18. Hoefert L. L., Pinto R. L., Fail G. L. 1988; Ultrastructural effects of Lettuce infectious yellows virus in Lactuca sativa L. J Ultrastruct Mol Struct Res 98:243–253 [CrossRef]
    [Google Scholar]
  19. Karasev A. V. 2000; Genetic diversity and evolution of closteroviruses. Annu Rev Phytopathol 38:293–324 [CrossRef]
    [Google Scholar]
  20. Klaassen V. A., Boeshore M., Dolja V. V., Falk B. W. 1994; Partial characterization of the lettuce infectious yellows virus genomic RNAs, identification of the coat protein gene and comparison of its amino acid sequence with those of other filamentous RNA plant viruses. J Gen Virol 75:1525–1533 [CrossRef]
    [Google Scholar]
  21. Klaassen V. A., Mayhew D., Fisher D., Falk B. W. 1996; In vitro transcripts from cloned cDNAs of the lettuce infectious yellows closterovirus bipartite genomic RNAs are competent for replication in Nicotiana benthamiana protoplasts. Virology 222:169–175 [CrossRef]
    [Google Scholar]
  22. Knapp E., Dawson W. O., Lewandowski D. J. 2001; Conundrum of the lack of defective RNAs (dRNAs) associated with tobamovirus infections: dRNAs that can move are not replicated by the wild-type virus; dRNAs that are replicated by the wild-type virus do not move. J Virol 75:5518–5525 [CrossRef]
    [Google Scholar]
  23. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685 [CrossRef]
    [Google Scholar]
  24. Leh V., Jacquot E., Geldreich A., Hermann T., Leclerc D., Cerutti M., Yot P., Keller M., Blanc S. 1999; Aphid transmission of cauliflower mosaic virus requires the viral PIII protein. EMBO J 18:7077–7085 [CrossRef]
    [Google Scholar]
  25. Lindbo J. A., Silva-Rosales L., Proebsting W. M., Dougherty W. G. 1993; Induction of a highly specific antiviral state in transgenic plants: implications for regulation of gene expression and virus resistance. Plant Cell 5:1749–1759 [CrossRef]
    [Google Scholar]
  26. MacFarlane S. A. 2003; Molecular determinants of the transmission of plant viruses by nematodes. Mol Plant Pathol 4:211–215 [CrossRef]
    [Google Scholar]
  27. Martelli G. P., Agranovsky A. A., Bar-Joseph M. 13 other authors 2002; The family Closteroviridae revised. Arch Virol 147:2039–2044 [CrossRef]
    [Google Scholar]
  28. Mawassi M., Karasev A. V., Mietkiewska E., Gafny R., Lee R. F., Dawson W. O., Bar-Joseph M. 1995a; Defective RNA molecules associated with citrus tristeza virus. Virology 208:383–387 [CrossRef]
    [Google Scholar]
  29. Mawassi M., Mietkiewska E., Hilf M. E. & 7 other authors (1995b). Multiple species of defective RNAs in plants infected with citrus tristeza virus. Virology 214:264–268 [CrossRef]
    [Google Scholar]
  30. Medina V., Rodrigo G., Tian T., Juarez M., Dolja V. V., Achon M. A., Falk B. W. 2003; Comparative cytopathology of Crinivirus infections in different plant hosts. Ann Appl Biol 143:99–110 [CrossRef]
    [Google Scholar]
  31. Napuli A. J., Alzhanova D. V., Doneanu C. E., Barofsky D. F., Koonin E. V., Dolja V. V. 2003; The 64-kilodalton capsid protein homolog of Beet yellows virus is required for assembly of virion tails. J Virol 77:2377–2384 [CrossRef]
    [Google Scholar]
  32. Ng J., Perry K. L. 1999; Stability of the aphid transmission phenotype in cucumber mosaic virus. Plant Pathol 48:388–394 [CrossRef]
    [Google Scholar]
  33. Passmore B. K., Sanger M., Chin L. S., Falk B. W., Bruening G. 1993; Beet western yellows virus-associated RNA: an independently replicating RNA that stimulates virus accumulation. Proc Natl Acad Sci U S A 90:10168–10172 [CrossRef]
    [Google Scholar]
  34. Peng Y. H., Kadoury D., Gal-On A., Huet H., Wang Y., Raccah B. 1998; Mutations in the HC-Pro gene of zucchini yellow mosaic potyvirus: effects on aphid transmission and binding to purified virions. J Gen Virol 79:897–904
    [Google Scholar]
  35. Perring T. M., Cooper A. D., Rodriguez R. J., Farrar C. A., Bellows T. S. 1993; Identification of a whitefly species by genomic and behavioral studies. Science 259:74–77 [CrossRef]
    [Google Scholar]
  36. Pirone T. P., Blanc S. 1996; Helper-dependent transmission of plant viruses. Annu Rev Phytopathol 34:227–247 [CrossRef]
    [Google Scholar]
  37. Pirone T. P., Megahed E. 1966; Aphid transmissibility of some purified viruses and viral RNAs. Virology 30:631–637 [CrossRef]
    [Google Scholar]
  38. Rabindran S., Dawson W. O. 2001; Assessment of recombinants that arise from the use of a TMV-based transient expression vector. Virology 284:182–189 [CrossRef]
    [Google Scholar]
  39. Rochow W. F. 1970; Barley yellow dwarf virus : phenotypic mixing and vector specificity. Science 167:875–878 [CrossRef]
    [Google Scholar]
  40. Rouze-Jouan J., Terradot L., Pasquer F., Tanguy S., Giblot Ducray-Bourdin D. D. 2001; The passage of Potato leafroll virus through Myzus persicae gut membrane regulates transmission efficiency. J Gen Virol 82:17–23
    [Google Scholar]
  41. Rubio L., Yeh H. H., Tian T., Falk B. W. 2000; A heterogeneous population of defective RNAs is associated with lettuce infectious yellows virus. Virology 271:205–212 [CrossRef]
    [Google Scholar]
  42. Rubio L., Tian T., Yeh H. H., Livieratos Y., Falk B. W. 2002; De novo generation of Lettuce infectious yellows virus defective RNAs in protoplasts. Mol Plant Pathol 3:321–327 [CrossRef]
    [Google Scholar]
  43. Sanger M., Passmore B., Falk B. W., Bruening G., Ding B., Lucas W. J. 1994; Symptom severity of beet western yellows virus strain ST9 is conferred by the ST9-associated RNA and is not associated with virus release from the phloem. Virology 200:48–55 [CrossRef]
    [Google Scholar]
  44. Satyanarayana T., Gowda S., Ayllon M. A., Dawson W. O. 2004; Closterovirus bipolar virion: evidence for initiation of assembly by minor coat protein and its restriction to the genomic RNA 5′ region. Proc Natl Acad Sci U S A 101:799–804 [CrossRef]
    [Google Scholar]
  45. Tian T., Rubio L., Yeh H. H., Crawford B., Falk B. W. 1999; Lettuce infectious yellows virus: in vitro acquisition analysis using partially purified virions and the whitefly Bemisia tabaci . J Gen Virol 80:1111–1117
    [Google Scholar]
  46. Wang R. Y., Ammuar E. D., Thornbury D. W., Lopez-Moya J. J., Pirone T. P. 1996; Loss of potyvirus transmissibility and helper-component activity correlate with non-retention of virions in aphid stylets. J Gen Virol 77:861–867 [CrossRef]
    [Google Scholar]
  47. Yang G., Mawassi M., Ashoulin L., Gafny R., Gaba V., Gal-On A., Bar-Joseph M. 1997; A cDNA clone from a defective RNA of citrus tristeza virus is infective in the presence of the helper virus. J Gen Virol 78:1765–1769
    [Google Scholar]
  48. Yeh H. H., Tian T., Rubio L., Crawford B., Falk B. W. 2000; Asynchronous accumulation of lettuce infectious yellows virus RNAs 1 and 2 and identification of an RNA 1 trans enhancer of RNA 2 accumulation. J Virol 74:5762–5768 [CrossRef]
    [Google Scholar]
  49. Yeh H. H., Tian T., Medina V., Falk B. W. 2001; Green fluorescent protein expression from recombinant lettuce infectious yellows virus-defective RNAs originating from RNA 2. Virology 289:54–62 [CrossRef]
    [Google Scholar]
  50. Zinovkin R. A., Jelkmann W., Agranovsky A. A. 1999; The minor coat protein of beet yellows closterovirus encapsidates the 5′ terminus of RNA in virions. J Gen Virol 80:269–272
    [Google Scholar]
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