Virus entry or the primary infection cycle are not the principal determinants of host specificity of spp. nucleopolyhedroviruses Free

Abstract

The multicapsid nucleopolyhedroviruses (NPVs) of (SeMNPV), (SfMNPV), and (SpliNPV) are genetically similar (78 % similarity) but differ in their degree of host specificity. Infection by each of the three NPVs in these three host species was determined by oral inoculation of larvae with occlusion bodies (OBs) or intrahaemocoelic injection with occlusion derived virions (ODVs). RT-PCR analysis of total RNA from inoculated insects, targeted at immediate early (), early (, DNA polymerase), late (chitinase) and very late genes (polyhedrin), indicated that each of the NPVs initiated an infection in all three host species tested. SpliMNPV produced a fatal NPV disease in both heterologous hosts, and , by oral inoculation or injection. SfMNPV was lethal to heterologous hosts, and , but infected larvae did not melt and disintegrate, and progeny OBs were not observed. SeMNPV was able to replicate in heterologous hosts and all genes required for replication were present in the genome, as the virus primary infection cycle was observed. However, gene expression was significantly lower in heterologous hosts. SeMNPV pathogenesis in and was blocked at the haemocoel transmission stage and very nearly cleared. SeMNPV mixtures with SpliMNPV or SfMNPV did not extend the host range of SeMNPV; in all cases, only the homologous virus was observed to proliferate. It is concluded that entry and the primary virus infection cycle are not the only, or the major determinants, for SeMNPV infection of heterologous species.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.80179-0
2004-10-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/85/10/vir852845.html?itemId=/content/journal/jgv/10.1099/vir.0.80179-0&mimeType=html&fmt=ahah

References

  1. Brown S. E., Delhurst C. F. 1975; The genus Spodoptera (Lepidoptera: Noctuidae) in Africa and the Near East. Bull Entomol Res 65:221–262 [CrossRef]
    [Google Scholar]
  2. Caballero P., Zuidema D., Santiago-Alvarez C., Vlak J. M. 1992; Biochemical and biological characterization of four isolates of Spodoptera exigua nuclear polyhedrosis virus. Biocontrol Sci Technol 2:145–157 [CrossRef]
    [Google Scholar]
  3. Chakraborty S., Monsour C., Teakle R., Reid S. 1999; Yield, biological activity, and field performance of a wild-type Helicoverpa nucleopolyhedrovirus produced in H. zea cell cultures. J Invertebr Pathol 73:199–205 [CrossRef]
    [Google Scholar]
  4. Clem R. J. 2001; Baculoviruses and apoptosis: the good, the bad, and the ugly. Cell Death Differ 8:137–143 [CrossRef]
    [Google Scholar]
  5. Clem R. J., Miller L. K. 1993; Apoptosis reduces both the in vitro replication and the in vivo infectivity of a baculovirus. J Virol 67:3730–3738
    [Google Scholar]
  6. Croizier G., Ribeiro H. C. T. 1992; Recombination as a possible major cause of genetic heterogeneity in Anticarsia gemmatalis nuclear polyhedrosis virus wild populations. Virus Res 26:183–196 [CrossRef]
    [Google Scholar]
  7. Croizier G., Boukhoudmi-Amiri K., Croizier L. 1989; A physical map of Spodoptera littoralis B-type nuclear polyhedrosis virus genome. Arch Virol 104:145–151 [CrossRef]
    [Google Scholar]
  8. Croizier G., Croizier L., Argaud O., Poudevigne D. 1994; Extension of Autographa californica nuclear polyhedrosis virus host range by interspecific replacement of a short DNA sequence in the p143 helicase gene. Proc Natl Acad Sci U S A 91:48–52 [CrossRef]
    [Google Scholar]
  9. Escribano A., Williams T., Goulson D., Cave R. D., Chapman J. W., Caballero P. 1999; Selection of a nucleopolyhedrovirus for control of Spodoptera frugiperda (Lepidoptera: Noctuidae): structural, genetic, and biological comparison of four isolates from the Americas. J Econ Entomol 92:1079–1085 [CrossRef]
    [Google Scholar]
  10. Faktor O., Toister-Achituv M., Kamensky B. 1995; Identification and nucleotide sequence of an ecdysteroid UDP-glucosyltransferase gene of Spodoptera littoralis multicapsid nuclear polyhedrosis virus. Virus Genes 11:47–52 [CrossRef]
    [Google Scholar]
  11. Gelernter W. D., Federici B. A. 1986; Isolation, identification and determination of virulence of a nuclear polyhedrosis virus from the beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae). Environ Entomol 15:240–245 [CrossRef]
    [Google Scholar]
  12. Greene G. L., Leppla N. C., Dickerson W. A. 1976; Velvetbean caterpillar: a rearing procedure and artificial medium. J Econ Entomol 69:487–488 [CrossRef]
    [Google Scholar]
  13. Gröner A. 1986; Specificity and safety of baculoviruses. In The Biology of Baculoviruses . Biological Properties and Molecular Biology pp  177–202 Edited by Granados R. R., Federici B. A. San Diego: Academic Press;
    [Google Scholar]
  14. Heldens J. G., Broer R., Zuidema D., Goldbach R. W., Vlak J. M. 1997; Identification and functional analysis of a non- hr origin of DNA replication in the genome of Spodoptera exigua multicapsid nucleopolyhedrovirus. J Gen Virol 78:1497–1506
    [Google Scholar]
  15. Hughes P. R., Wood H. A. 1981; A synchronous peroral technique for the bioassay of insect viruses. J Invertebr Pathol 37:154–159 [CrossRef]
    [Google Scholar]
  16. Ijkel W. F., van Strien E. A., Heldens J. G., Broer R., Zuidema D., Goldbach R. W., Vlak J. M. 1999; Sequence and organization of the Spodoptera exigua multicapsid nucleopolyhedrovirus genome. J Gen Virol 80:3289–3304
    [Google Scholar]
  17. Kamita S. G., Maeda S., Hammock B. D. 2003; High-frequency homologous recombination between baculoviruses involves DNA replication. J Virol 77:13053–13061 [CrossRef]
    [Google Scholar]
  18. Kondo A., Maeda S. 1991; Host range expansion by recombination of the baculoviruses Bombyx mori nuclear polyhedrosis virus and Autographa californica nuclear polyhedrosis virus. J Virol 65:3625–3632
    [Google Scholar]
  19. Kool M., Ahrens C. H., Goldbach R. W., Rohrmann G. F., Vlak J. M. 1994; Identification of genes involved in DNA replication of the Autographa californica baculovirus. Proc Natl Acad Sci U S A 91:11212–11216 [CrossRef]
    [Google Scholar]
  20. LaCount D. J., Friesen P. D. 1997; Role of early and late replication events in induction of apoptosis by baculoviruses. J Virol 71:1530–1537
    [Google Scholar]
  21. López-Ferber M., Simon O., Williams T., Caballero P. 2003; Defective or effective? Mutualistic interactions between virus genotypes. Proc R Soc Lond B Biol Sci 270:2249–2255 [CrossRef]
    [Google Scholar]
  22. Lu A., Miller L. K. 1997; Regulation of baculoviruses late and very late gene expression. In The Baculoviruses pp  193–216 Edited by Miller L. K. New York: Plenum;
    [Google Scholar]
  23. Maeda S., Kamita S. G., Kondo A. 1993; Host range expansion of Autographa californica nuclear polyhedrosis virus (NPV) following recombination of a 0·6-kilobase-pair DNA fragment originating from Bombyx mori NPV. J Virol 67:6234–6238
    [Google Scholar]
  24. Martin O., Croizier G. 1997; Infection of a Spodoptera frugiperda cell line with Bombyx mori nucleopolyhedrovirus. Virus Res 47:179–185 [CrossRef]
    [Google Scholar]
  25. Miller L. K. 1997; Baculovirus interaction with host apoptotic pathways. J Cell Physiol 173:178–182 [CrossRef]
    [Google Scholar]
  26. Miller L. K., Lu A. 1997; The molecular basis of baculovirus host range. In The Baculoviruses pp  217–235 Edited by Miller L. K. New York: Plenum;
    [Google Scholar]
  27. Monsma S. A., Oomens A. G., Blissard G. W. 1996; The GP64 envelope fusion protein is an essential baculovirus protein required for cell-to-cell transmission of infection. J Virol 70:4607–4616
    [Google Scholar]
  28. Moscardi F. 1999; Assessment of the application of baculoviruses for control of Lepidoptera. Annu Rev Entomol 44:257–289 [CrossRef]
    [Google Scholar]
  29. Muñoz D., Castillejo J. I., Caballero P. 1998; Naturally occurring deletion mutants are parasitic genotypes in a wild-type nucleopolyhedrovirus population of Spodoptera exigua . Appl Environ Microbiol 64:4372–4377
    [Google Scholar]
  30. Murillo R., Muñoz D., Caballero P. 2003; Host range and biological activity of three Spodoptera nucleopolyhedrovirus genotypic variants and the effect of Tinopal LPW on the most active variant. Int J Pest Manag 49:147–153 [CrossRef]
    [Google Scholar]
  31. Prikhod'ko E. A., Miller L. K. 1996; Induction of apoptosis by baculovirus transactivator IE1. J Virol 70:7116–7124
    [Google Scholar]
  32. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual , 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  33. Simón O., Williams T., López-Ferber M., Caballero P. 2004; Structure of a Spodoptera frugiperda nucleopolyehdrovirus population: high prevalence of per os non-infective deletion mutants. Appl Environ Microbiol 70 (in press
    [Google Scholar]
  34. Thiem S. M. 1997; Prospects for altering host range for baculovirus bioinsecticides. Curr Opin Biotechnol 8:317–322 [CrossRef]
    [Google Scholar]
  35. Thiem S. M., Du X., Quentin M. E., Berner M. M. 1996; Identification of baculovirus gene that promotes Autographa californica nuclear polyhedrosis virus replication in a nonpermissive insect cell line. J Virol 70:2221–2229
    [Google Scholar]
  36. Tumilasci V. F., Leal E., Zanotto P. M., Luque T., Wolff J. L. 2003; Sequence analysis of a 5·1 kbp region of the Spodoptera frugiperda multicapsid nucleopolyhedrovirus genome that comprises a functional ecdysteroid UDP-glucosyltransferase ( egt ) gene. Virus Genes 27:137–144 [CrossRef]
    [Google Scholar]
  37. Washburn J. O., Kirkpatrick B. A., Volkman L. E. 1996; Insect protection against viruses. Nature 383:767 [CrossRef]
    [Google Scholar]
  38. Washburn J. O., Haas-Stapleton E. J., Tan F. F., Beckage N. E., Volkman L. E. 2000; Co-infection of Manduca sexta larvae with polydnavirus from Cotesia congregata increases susceptibility to fatal infection by Autographa californica M Nucleopolyhedrovirus. J Insect Physiol 46:179–190 [CrossRef]
    [Google Scholar]
  39. Yanase T., Yasunaga C., Hara T., Kawarabata T. 1998a; Coinfection of Spodoptera exigua and Spodoptera frugiperda cell lines with the nuclear polyhedrosis viruses of Autographa californica and Spodoptera exigua . Intervirology 41:244–252 [CrossRef]
    [Google Scholar]
  40. Yanase T., Yasunaga C., Kawarabata T. 1998b; Replication of Spodoptera exigua nucleopolyhedrovirus in permissive and non-permissive lepidopteran cell lines. Acta Virol 42:293–298
    [Google Scholar]
  41. Zhang P., Yang K., Dai X., Pang Y., Su D. 2002; Infection of wild-type Autographa californica multicapsid nucleopolyhedrovirus induces in vivo apoptosis of Spodoptera litura larvae. J Gen Virol 83:3003–3011
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.80179-0
Loading
/content/journal/jgv/10.1099/vir.0.80179-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed