RT Journal Article SR Electronic(1) A1 Chang, Yun-Shiang A1 Peng, Shao-En A1 Yu, Hon-Tsen A1 Liu, Feng-Chuan A1 Wang, Chung-Hsiung A1 Lo, Chu-Fang A1 Kou, Guang-HsiungYR 2004 T1 Genetic and phenotypic variations of isolates of shrimp Taura syndrome virus found in Penaeus monodon and Metapenaeus ensis in Taiwan JF Journal of General Virology, VO 85 IS 10 SP 2963 OP 2968 DO https://doi.org/10.1099/vir.0.80132-0 PB Microbiology Society, SN 1465-2099, AB Distinct Taura syndrome virus (TSV) isolates were found in Metapenaeus ensis (isolate Tw2KMeTSV), Penaeus monodon (isolate Tw2KPmTSV) and Litopenaeus vannamei (isolate Tw02LvTSV). Nucleotide sequence analysis of these three isolates revealed differences in the TSV structural protein (capsid protein precursor) gene orf2. TSV ORF2 amino acid sequence comparison and phylogenetic analysis suggested a comparatively close relationship between these three Taiwanese isolates and the Hawaiian isolate HI94TSV. In P. monodon specimens that were naturally and experimentally infected with the Tw2KPmTSV isolate, the virus was contained and shrimps showed no clinical signs of infection. However, when P. monodon was challenged with the Tw2KMeTSV isolate, the virus replicated freely. The ORF2 amino acid sequence of the Tw2KMeTSV isolate differed from that of isolate Tw2KPmTSV in four positions and these differences may account for their phenotypic differences, at least in terms of their ability to replicate in specific hosts., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.80132-0