%0 Journal Article %A Gómez-Sebastián, S. %A Tabarés, E. %T Negative regulation of herpes simplex virus type 1 ICP4 promoter by IE180 protein of pseudorabies virus %D 2004 %J Journal of General Virology, %V 85 %N 8 %P 2125-2130 %@ 1465-2099 %R https://doi.org/10.1099/vir.0.80119-0 %I Microbiology Society, %X Recombinant pseudorabies viruses (PRVs) gIS8 and N1aHTK were constructed by the insertion of a chimeric gene (α4–TK) from herpes simplex virus type 1 (HSV-1) into wild-type PRV. HSV-1 TK expression by these recombinant viruses resulted in enhanced sensitivity to ganciclovir, compared to that of the wild-type PRV, and was similar to the sensitivity shown by HSV-1. Infection with gIS8 or N1aHTK recombinant viruses led to expression of HSV-1 TK mRNA as an immediate–early (IE) gene, observed by downregulation of the HSV-1 α4 promoter. This negative regulation was due to a PRV IE protein, IE180. IE180, however, does not have all the regulatory functions of the infected-cell protein ICP4, as it does not restore the growth of ICP4-deficient HSV-1 mutants. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.80119-0