1887

Abstract

The accumulation of cellular proliferating cell nuclear antigen (PCNA) in the nucleus of Sf9 cells has been shown to increase upon infection with multicapsid nucleopolyhedrovirus (AcMNPV). Here, analysis by DNase I treatment and chromatin immunoprecipitation revealed that cellular PCNA in the nucleus of Sf9 cells bound AcMNPV DNA. Immunocytochemical analysis showed colocalization of Sf9 cell PCNA and AcMNPV DNA replication sites. Similar colocalization was also observed in BmN-4 cells infected with NPV, which is inherently missing the gene. The amount of cellular PCNA associated with viral DNA replication sites was greater in cells infected with -defective AcMNPV mutants than in cells infected with wild-type AcMNPV. These results suggest that both cellular and viral PCNAs are involved in AcMNPV DNA replication and that -defective AcMNPV mutants are able to substitute cellular PCNA for viral PCNA.

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2004-10-01
2024-12-07
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References

  1. Ayres M. D., Howard S. C., Kuzio J., Lopez-Ferber M., Possee R. D. 1994; The complete DNA sequence of Autographa californica nuclear polyhedrosis virus. Virology 202:586–605 [CrossRef]
    [Google Scholar]
  2. Chaeychomsri S., Ikeda M., Kobayashi M. 1995; Nucleotide sequence and transcriptional analysis of the DNA polymerase gene of Bombyx mori nuclear polyhedrosis virus. Virology 206:435–447 [CrossRef]
    [Google Scholar]
  3. Crawford A. M., Miller L. K. 1988; Characterization of an early gene accelerating expression of late genes of the baculovirus Autographa californica nuclear polyhedrosis virus. J Virol 62:2773–2781
    [Google Scholar]
  4. Fujita M., Kiyono T., Hayashi Y., Ishibashi M. 1997; In vivo interaction of human MCM heterohexameric complexes with chromatin. J Biol Chem 272:10928–10935 [CrossRef]
    [Google Scholar]
  5. Gomi S., Majima K., Maeda S. 1999; Sequence analysis of the genome of Bombyx mori nucleopolyhedrovirus. J Gen Virol 80:1323–1337
    [Google Scholar]
  6. Iwahori S., Ikeda M., Kobayashi M. 2002a; Comparative characterization of pcna genes from Hyphantria cunea nucleopolyhedrovirus, and two cell lines from the fall armyworm, Spodoptera frugiperda , and the fall webworm, Hyphantria cunea . J Insect Biotechnol Sericol 71:25–34
    [Google Scholar]
  7. Iwahori S., Ikeda M., Kobayashi M. 2002b; Generation and characterization of Autographa californica nucleopolyhedrovirus mutants defective in pcna gene homologue. J Insect Biotechnol Sericol 71:129–139
    [Google Scholar]
  8. Kool M., Ahrens C. H., Goldbach R. W., Rohrmann G. F., Vlak J. M. 1994; Identification of genes involved in DNA replication of the Autographa californica baculovirus. Proc Natl Acad Sci U S A 91:11212–11216 [CrossRef]
    [Google Scholar]
  9. Ladenburger E.-M., Keller C., Knippers R. 2002; Identification of a binding region for human origin recognition complex proteins 1 and 2 that coincides with an origin of DNA replication. Mol Cell Biol 22:1036–1048 [CrossRef]
    [Google Scholar]
  10. Lee H. H., Miller L. K. 1978; Isolation of genotypic variants of Autographa californica nuclear polyhedrosis virus. J Virol 27:754–767
    [Google Scholar]
  11. Lu A., Miller L. K. 1995; The roles of eighteen baculovirus late expression factor genes in transcription and DNA replication. J Virol 69:975–982
    [Google Scholar]
  12. Lu A., Krell P. J., Vlak J. M., Rohrmann G. F. 1997; Baculovirus DNA replication. In The Baculoviruses pp  171–191 Edited by Miller L. K. New York: Plenum;
    [Google Scholar]
  13. Maeda S. 1989; Gene transfer vector of a baculovirus, Bombyx mori , and their use for expression of foreign genes in insect cells. In Invertebrate Cell System Applications pp  167–181 Edited by Mitsuhashi J. Boca Raton, FL: CRC Press;
    [Google Scholar]
  14. Mainz D., Quadt I., Knebel-Mörsdorf D. 2002; Nuclear IE2 structures are related to viral DNA replication sites during baculovirus infection. J Virol 76:5198–5207 [CrossRef]
    [Google Scholar]
  15. McDougal V. V., Guarino L. A. 1999; Autographa californica nuclear polyhedrosis virus DNA polymerase: measurements of processivity and strand displacement. J Virol 73:4908–4918
    [Google Scholar]
  16. Mikhailov V. S., Rohrmann G. F. 2002; Baculovirus replication factor LEF-1 is a DNA primase. J Virol 76:2287–2297 [CrossRef]
    [Google Scholar]
  17. Mikhailov V. S., Marlyev K. A., Ataeva J. O., Kullyev P. K., Atrazhev A. M. 1986; Characterization of 3′→5′ exonuclease associated with DNA polymerase of silkworm nuclear polyhedrosis virus. Nucleic Acids Res 14:3841–3857 [CrossRef]
    [Google Scholar]
  18. Miller L. K., Jewell J. E., Browne D. 1981; Baculovirus induction of a DNA polymerase. J Virol 40:305–308
    [Google Scholar]
  19. Mitsuhashi J., Inoue H. 1988; Obtainment of a continuous cell line from the larval fat bodies of the mulberry tiger moth, Spilosoma imparilis (Lepidoptera: Arctiidae). Appl Entomol Zool 23:488–490
    [Google Scholar]
  20. Nagamine T., Shimomura M., Sugimori H., Kobayashi M. 1989; Titration of Bombyx mori (Lepidoptera: Bombycidae) nuclear polyhedrosis virus in a Bombyx mori cell line. Appl Entomol Zool 24:235–237
    [Google Scholar]
  21. Ohzeki J., Nakano M., Okada T., Masumoto H. 2002; CENP-B box is required for de novo centromere chromatin assembly on human alphoid DNA. J Cell Biol 159:765–775 [CrossRef]
    [Google Scholar]
  22. Okano K., Mikhailov V. S., Maeda S. 1999; Colocalization of baculovirus IE-1 and two DNA-binding proteins, DBP and LEF-3, to viral replication factories. J Virol 73:110–119
    [Google Scholar]
  23. O'Reilly D. R., Crawford A. M., Miller L. K. 1989; Viral proliferating cell nuclear antigen. Nature 337:606
    [Google Scholar]
  24. Schepers A., Ritzi M., Bousset K., Kremmer E., Yates J. L., Harwood J., Diffley J. F. X., Hammerschmidt W. 2001; Human origin recognition complex binds to the region of the latent origin of DNA replication of Epstein–Barr virus. EMBO J 20:4588–4602 [CrossRef]
    [Google Scholar]
  25. Tsurimoto T. 1998; PCNA, a multifunctional ring on DNA. Biochim Biophys Acta 1443:23–39 [CrossRef]
    [Google Scholar]
  26. Tsurimoto T. 1999; PCNA binding proteins. Front Biosci 4:849–858 [CrossRef]
    [Google Scholar]
  27. Wang X., Kelly D. C. 1983; Baculovirus replication: purification and identification of the Trichoplusia ni nuclear polyhedrosis virus-induced DNA polymerase. J Gen Virol 64:2229–2236 [CrossRef]
    [Google Scholar]
  28. Waseem N. H., Lane D. P. 1990; Monoclonal antibody analysis of the proliferating cell nuclear antigen (PCNA): structural conservation and the detection of a nucleolar form. J Cell Sci 96:121–129
    [Google Scholar]
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