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Feline immunodeficiency virus (FIV) infection in cats is associated with an increase of feline CD (fCD)8α + β − and fCD8α + β low cells in peripheral blood. To investigate these cells in more detail, an anti-fCD3ε mAb, termed NZM1, was generated, which recognizes the extracellular epitope of the fCD3ε molecule. The anti-fCD3ε mAb proved to be more suitable for identifying feline T cells than the anti-fCD5 one, which has been used as a pan-T-cell reagent in cats, because of the presence of fCD5+fCD3ε − cells among lymphocytes. Although the fCD8α + β − and fCD8α + β low cells in the FIV-infected cats expressed fCD3ε, a subset of fCD8α + β − cells expressed fCD3ε antigen at a lower level than the T cells whose phenotype was fCD4+, or fCD8α + β low. The lower expression of fCD3ε may be associated with the immune status of fCD8α + β − T cells.
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