1887

Abstract

The capsid of birnaviruses contains two proteins, VP2 and VP3, which derive from the processing of a large polyprotein, NH–pVP2–VP4–VP3–COOH. The proteolytic cascade involved in processing the polyprotein, and in the final maturation of pVP2 (the precursor of VP2), has recently been shown to generate VP2 and four structural peptides in infectious bursal disease virus and blotched snakehead virus. The presence of peptides in infectious pancreatic necrosis virus particles was investigated using mass spectrometry and N-terminal sequencing of virus particles. Three peptides deriving from the C terminus of pVP2 (residues 443–486, 487–495 and 496–508 of the polyprotein) and 14 additional peptides produced by further processing of peptides [443–486] and [496–508] were identified. These results indicate that the presence of several virus-encoded peptides in the virions is a hallmark of birnaviruses.

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2004-08-01
2019-11-22
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References

  1. Birghan, C., Mundt, E. & Gorbalenya, A. E. ( 2000; ). A non-canonical Lon proteinase lacking the ATPase domain employs the Ser–Lys catalytic dyad to exercise broad control over the life cycle of a double-stranded RNA virus. EMBO J 19, 114–123.[CrossRef]
    [Google Scholar]
  2. Böttcher, B., Kiselev, N. A., Stel'Mashchuk, V. Y., Perevozchikova, N. A., Borisov, A. V. & Crowther, R. A. ( 1997; ). Three-dimensional structure of infectious bursal disease virus determined by electron cryomicroscopy. J Virol 71, 325–330.
    [Google Scholar]
  3. Caston, J. R., Martinez-Torrecuadrada, J. L., Maraver, A., Lombardo, E., Rodriguez, J. F., Casal, J. I. & Carrascosa, J. L. ( 2001; ). C terminus of infectious bursal disease virus major capsid protein VP2 is involved in definition of the T number for capsid assembly. J Virol 75, 10815–10828.[CrossRef]
    [Google Scholar]
  4. Chevalier, C., Lepault, J., Erk, I., Da Costa, B. & Delmas, B. ( 2002; ). The maturation process of pVP2 requires assembly of infectious bursal disease virus capsids. J Virol 76, 2384–2392.[CrossRef]
    [Google Scholar]
  5. Chung, H. K., Kordyban, S., Cameron, L. & Dobos, P. ( 1996; ). Sequence analysis of the bicistronic drosophila X virus genome segment A and its encoded polypeptides. Virology 225, 359–368.[CrossRef]
    [Google Scholar]
  6. Da Costa, B., Chevalier, C., Henry, C., Huet, J.-C., Petit, S., Lepault, J., Boot, H. & Delmas, B. ( 2002; ). The capsid of infectious bursal disease virus contains several small peptides arising from the maturation process of pVP2. J Virol 76, 2393–2402.[CrossRef]
    [Google Scholar]
  7. Da Costa, B., Soignier, S., Chevalier, C., Henry, C., Thory, C., Huet, J.-C. & Delmas, B. ( 2003; ). Blotched snakehead virus is a new aquatic birnavirus that is slightly more related to avibirnavirus than to aquabirnavirus. J Virol 77, 719–725.[CrossRef]
    [Google Scholar]
  8. Dobos, P. ( 1995; ). The molecular biology of infectious pancreatic necrosis virus (IPNV). Annu Rev Fish Dis 5, 25–54.[CrossRef]
    [Google Scholar]
  9. Dobos, P., Hill, B. J., Hallett, R., Kells, D. T., Becht, H. & Teninges, D. ( 1979; ). Biophysical and biochemical characterization of five animal viruses with bisegmented double-stranded RNA genomes. J Virol 32, 593–605.
    [Google Scholar]
  10. Dorson, M., Castric, J. & Torchy, C. ( 1978; ). Infectious pancreatic necrosis virus of salmonids: biological and antigenic features of a pathogenic strain and a nonpathogenic variant selected in RTG-2 cells. J Fish Dis 1, 309–320.[CrossRef]
    [Google Scholar]
  11. Gorbalenya, A. E., Pringle, F. M., Zeddam, J. L., Luke, B. T., Cameron, C. E., Kalmakoff, J., Hanzlik, T. N., Gordon, K. H. & Ward, V. K. ( 2002; ). The palm subdomain-based active site is internally permuted in viral RNA-dependent RNA polymerases of an ancient lineage. J Mol Biol 324, 47–62.[CrossRef]
    [Google Scholar]
  12. John, K. R. & Richards, R. H. ( 1999; ). Characteristics of a new birnavirus associated with a warm-water fish cell line. J Gen Virol 80, 2061–2065.
    [Google Scholar]
  13. Kibenge, F. S. B., Dhillon, A. S. & Russell, R. G. ( 1988; ). Biochemistry and immunology of infectious bursal disease virus. J Gen Virol 69, 1757–1775.[CrossRef]
    [Google Scholar]
  14. Lejal, N., Da Costa, B., Huet, J.-C. & Delmas, B. ( 2000; ). Role of Ser-652 and Lys-692 in the protease activity of infectious bursal disease virus VP4 and identification of its substrate cleavage sites. J Gen Virol 81, 983–992.
    [Google Scholar]
  15. Leong, J. C., Brown, D., Dobos, P., Kibenge, F. S. B., Ludert, J. E., Müller, H., Mundt, E. & Nicholson, B. ( 2000; ). Family Birnaviridae. In Virus Taxonomy. Seventh Report of the International Committee on Taxonomy of Viruses, pp. 481–490. Edited by M. H. V. van Regenmortel, C. M. Fauquet, D. H. L. Bishop, E. B. Carstens, M. K. Estes, S. M. Lemon, J. Maniloff, M. A. Mayo, D. J. McGeoch, C. R. Pringle & R. B. Wickner. San Diego: Academic Press.
  16. Lombardo, E., Maraver, A., Caston, J. R., Rivera, J., Fernandez-Arias, A., Serrano, A., Carrascosa, J. L. & Rodriguez, J. F. ( 1999; ). VP1, the putative RNA-dependent RNA polymerase of infectious bursal disease virus, forms complexes with the capsid protein VP3, leading to efficient encapsidation into virus-like particles. J Virol 73, 6973–6983.
    [Google Scholar]
  17. Maraver, A., Clemente, R., Rodriguez, J. F. & Lombardo, E. ( 2003; ). Identification and molecular characterization of the RNA polymerase-binding motif of infectious bursal disease virus inner capsid protein VP3. J Virol 77, 2459–2468.[CrossRef]
    [Google Scholar]
  18. Martinez-Torrecuadrada, J. L., Caston, J. R., Castro, M., Carrascosa, J. L., Rodriguez, J. F. & Casal, J. I. ( 2000; ). Different architectures in the assembly of infectious bursal disease virus capsid proteins expressed in insect cells. Virology 278, 322–331.[CrossRef]
    [Google Scholar]
  19. Muller, H. & Becht, H. ( 1982; ). Biosynthesis of virus-specific proteins in cells infected with infectious bursal disease virus and their significance as structural elements for infectious virus and incomplete particles. J Virol 44, 384–392.
    [Google Scholar]
  20. Muller, H., Islam, M. R. & Raue, R. ( 2003; ). Research on infectious bursal disease – the past, the present and the future. Vet Microbiol 97, 153–165.[CrossRef]
    [Google Scholar]
  21. Mundt, E., Kollner, B. & Kretzschmar, D. ( 1997; ). VP5 of infectious bursal disease virus is not essential for viral replication in cell culture. J Virol 71, 5647–5651.
    [Google Scholar]
  22. Ozel, M. & Gelderblom, H. ( 1985; ). Capsid symmetry of viruses of the proposed Birnavirus group. Arch Virol 84, 149–161.[CrossRef]
    [Google Scholar]
  23. Petit, S., Lejal, N., Huet, J.-C. & Delmas, B. ( 2000; ). Active residues and viral substrate cleavage sites of the protease of the birnavirus infectious pancreatic necrosis virus. J Virol 74, 2057–2066.[CrossRef]
    [Google Scholar]
  24. Sanchez, A. B. & Rodriguez, J. F. ( 1999; ). Proteolytic processing in infectious bursal disease virus: identification of the polyprotein cleavage sites by site-directed mutagenesis. Virology 262, 190–199.[CrossRef]
    [Google Scholar]
  25. Tacken, M. G. J., Rottier, P. J. M., Gielkens, A. L. J. & Peeters, B. P. H. ( 2000; ). Interactions in vivo between the proteins of infectious bursal disease virus: capsid protein VP3 interacts with the RNA-dependent RNA polymerase, VP1. J Gen Virol 81, 209–218.
    [Google Scholar]
  26. Tacken, M. G., Peeters, B. P., Thomas, A. A., Rottier, P. J. & Boot, H. J. ( 2002; ). Infectious bursal disease virus capsid protein VP3 interacts both with VP1, the RNA-dependent RNA polymerase, and with viral double-stranded RNA. J Virol 76, 11301–11311.[CrossRef]
    [Google Scholar]
  27. Wolf, K., Snieszko, S. F., Dunbar, C. E. & Pyle, E. ( 1960; ). Virus nature of infectious pancreatic necrosis in trout. Proc Soc Exp Biol Med 104, 105–108.[CrossRef]
    [Google Scholar]
  28. Yao, K., Goodwin, M. A. & Vakharia, V. N. ( 1998; ). Generation of a mutant infectious bursal disease virus that does not cause bursal lesions. J Virol 72, 2647–2654.
    [Google Scholar]
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