1887

Abstract

Borna disease virus polymerase activity is negatively regulated by the viral X protein. Using a virus minireplicon system it was found that all X mutants that no longer interacted with the viral P protein failed to exhibit significant inhibitory activity. The action of X could further be neutralized by expression of a P fragment that contained the X interaction domain but lacked all domains known to mediate interaction with other viral proteins. X thus appears to regulate the activity of the Borna disease virus polymerase by targeting the polymerase cofactor P.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.80002-0
2004-07-01
2019-11-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/85/7/vir851895.html?itemId=/content/journal/jgv/10.1099/vir.0.80002-0&mimeType=html&fmt=ahah

References

  1. Cubitt, B. & de la Torre, J. C. ( 1994; ). Borna disease virus (BDV), a nonsegmented RNA virus, replicates in the nuclei of infected cells where infectious BDV ribonucleoproteins are present. J Virol 68, 1371–1381.
    [Google Scholar]
  2. de la Torre, J. C. ( 2002; ). Molecular biology of Borna disease virus and persistence. Front Biosci 7, d569–579.[CrossRef]
    [Google Scholar]
  3. Geib, T., Sauder, C., Venturelli, S., Hassler, C., Staeheli, P. & Schwemmle, M. ( 2003; ). Selective virus resistance conferred by expression of Borna disease virus nucleocapsid components. J Virol 77, 4283–4290.[CrossRef]
    [Google Scholar]
  4. Kobayashi, T., Shoya, Y., Koda, T., Takashima, I., Lai, P. K., Ikuta, K., Kakinuma, M. & Kishi, M. ( 1998; ). Nuclear targeting activity associated with the amino terminal region of the Borna disease virus nucleoprotein. Virology 243, 188–197.[CrossRef]
    [Google Scholar]
  5. Kobayashi, T., Zhang, G., Lee, B. J., Baba, S., Yamashita, M., Kamitani, W., Yanai, H., Tomonaga, K. & Ikuta, K. ( 2003; ). Modulation of Borna disease virus phosphoprotein nuclear localization by the viral protein X encoded in the overlapping open reading frame. J Virol 77, 8099–8107.[CrossRef]
    [Google Scholar]
  6. Nowotny, N., Kolodziejek, J., Jehle, C. O., Suchy, A., Staeheli, P. & Schwemmle, M. ( 2000; ). Isolation and characterization of a new subtype of Borna disease virus. J Virol 74, 5655–5658.[CrossRef]
    [Google Scholar]
  7. Perez, M., Sanchez, A., Cubitt, B., Rosario, D. & de la Torre, J. C. ( 2003; ). A reverse genetics system for Borna disease virus. J Gen Virol 84, 3099–3104.[CrossRef]
    [Google Scholar]
  8. Schneemann, A., Schneider, P. A., Lamb, R. A. & Lipkin, W. I. ( 1995; ). The remarkable coding strategy of Borna disease virus: a new member of the nonsegmented negative strand RNA viruses. Virology 210, 1–8.[CrossRef]
    [Google Scholar]
  9. Schneider, U., Naegele, M., Staeheli, P. & Schwemmle, M. ( 2003; ). Active Borna disease virus polymerase complex requires a distinct nucleoprotein-to-phosphoprotein ratio but no viral X protein. J Virol 77, 11781–11789.[CrossRef]
    [Google Scholar]
  10. Schwemmle, M., Salvatore, M., Shi, L., Richt, J., Lee, C. H. & Lipkin, W. I. ( 1998; ). Interactions of the Borna disease virus P, N, and X proteins and their functional implications. J Biol Chem 273, 9007–9012.[CrossRef]
    [Google Scholar]
  11. Staeheli, P., Sauder, C., Hausmann, J., Ehrensberger, F. & Schwemmle, M. ( 2000; ). Epidemiology of Borna disease virus. J Gen Virol 81, 2123–2135.
    [Google Scholar]
  12. Tomonaga, K., Kobayashi, T. & Ikuta, K. ( 2002; ). Molecular and cellular biology of Borna disease virus infection. Microbes Infect 4, 491–500.[CrossRef]
    [Google Scholar]
  13. Wolff, T., Pfleger, R., Wehner, T., Reinhardt, J. & Richt, J. A. ( 2000; ). A short leucine-rich sequence in the Borna disease virus p10 protein mediates association with the viral phospho- and nucleoproteins. J Gen Virol 81, 939–947.
    [Google Scholar]
  14. Wolff, T., Unterstab, G., Heins, G., Richt, J. A. & Kann, M. ( 2002; ). Characterization of an unusual importin alpha binding motif in the Borna disease virus p10 protein that directs nuclear import. J Biol Chem 277, 12151–12157.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.80002-0
Loading
/content/journal/jgv/10.1099/vir.0.80002-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error