1887

Abstract

Borna disease virus polymerase activity is negatively regulated by the viral X protein. Using a virus minireplicon system it was found that all X mutants that no longer interacted with the viral P protein failed to exhibit significant inhibitory activity. The action of X could further be neutralized by expression of a P fragment that contained the X interaction domain but lacked all domains known to mediate interaction with other viral proteins. X thus appears to regulate the activity of the Borna disease virus polymerase by targeting the polymerase cofactor P.

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2004-07-01
2021-03-07
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References

  1. Cubitt B., de la Torre J. C. 1994; Borna disease virus (BDV), a nonsegmented RNA virus, replicates in the nuclei of infected cells where infectious BDV ribonucleoproteins are present. J Virol 68:1371–1381
    [Google Scholar]
  2. de la Torre J. C. 2002; Molecular biology of Borna disease virus and persistence. Front Biosci 7:d569–579 [CrossRef]
    [Google Scholar]
  3. Geib T., Sauder C., Venturelli S., Hassler C., Staeheli P., Schwemmle M. 2003; Selective virus resistance conferred by expression of Borna disease virus nucleocapsid components. J Virol 77:4283–4290 [CrossRef]
    [Google Scholar]
  4. Kobayashi T., Shoya Y., Koda T., Takashima I., Lai P. K., Ikuta K., Kakinuma M., Kishi M. 1998; Nuclear targeting activity associated with the amino terminal region of the Borna disease virus nucleoprotein. Virology 243:188–197 [CrossRef]
    [Google Scholar]
  5. Kobayashi T., Zhang G., Lee B. J., Baba S., Yamashita M., Kamitani W., Yanai H., Tomonaga K., Ikuta K. 2003; Modulation of Borna disease virus phosphoprotein nuclear localization by the viral protein X encoded in the overlapping open reading frame. J Virol 77:8099–8107 [CrossRef]
    [Google Scholar]
  6. Nowotny N., Kolodziejek J., Jehle C. O., Suchy A., Staeheli P., Schwemmle M. 2000; Isolation and characterization of a new subtype of Borna disease virus. J Virol 74:5655–5658 [CrossRef]
    [Google Scholar]
  7. Perez M., Sanchez A., Cubitt B., Rosario D., de la Torre J. C. 2003; A reverse genetics system for Borna disease virus. J Gen Virol 84:3099–3104 [CrossRef]
    [Google Scholar]
  8. Schneemann A., Schneider P. A., Lamb R. A., Lipkin W. I. 1995; The remarkable coding strategy of Borna disease virus: a new member of the nonsegmented negative strand RNA viruses. Virology 210:1–8 [CrossRef]
    [Google Scholar]
  9. Schneider U., Naegele M., Staeheli P., Schwemmle M. 2003; Active Borna disease virus polymerase complex requires a distinct nucleoprotein-to-phosphoprotein ratio but no viral X protein. J Virol 77:11781–11789 [CrossRef]
    [Google Scholar]
  10. Schwemmle M., Salvatore M., Shi L., Richt J., Lee C. H., Lipkin W. I. 1998; Interactions of the Borna disease virus P, N, and X proteins and their functional implications. J Biol Chem 273:9007–9012 [CrossRef]
    [Google Scholar]
  11. Staeheli P., Sauder C., Hausmann J., Ehrensberger F., Schwemmle M. 2000; Epidemiology of Borna disease virus. J Gen Virol 81:2123–2135
    [Google Scholar]
  12. Tomonaga K., Kobayashi T., Ikuta K. 2002; Molecular and cellular biology of Borna disease virus infection. Microbes Infect 4:491–500 [CrossRef]
    [Google Scholar]
  13. Wolff T., Pfleger R., Wehner T., Reinhardt J., Richt J. A. 2000; A short leucine-rich sequence in the Borna disease virus p10 protein mediates association with the viral phospho- and nucleoproteins. J Gen Virol 81:939–947
    [Google Scholar]
  14. Wolff T., Unterstab G., Heins G., Richt J. A., Kann M. 2002; Characterization of an unusual importin alpha binding motif in the Borna disease virus p10 protein that directs nuclear import. J Biol Chem 277:12151–12157 [CrossRef]
    [Google Scholar]
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