1887

Abstract

Cap-independent internal initiation of translation occurs on a number of viral and cellular mRNAs and is directed by internal ribosome entry site (IRES) elements. virus (RhPV) is a member of the . These viruses have single-stranded, positive-sense RNA genomes that contain two open reading frames, both preceded by IRES elements. Previously, the activity of the RhPV 5′ UTR IRES has been demonstrated in mammalian, and wheat germ translation systems. It is now shown that this IRES also functions within (21) cells which are widely used in the baculovirus expression system, and in a novel 21 cell-based lysate system. Inclusion of the RhPV IRES in a dicistronic reporter mRNA transcript increased translation of the second cistron 23-fold within 21 cells. In contrast, the encephalomyocarditis virus IRES was inactive in both systems. The RhPV IRES therefore has the potential to be utilized in insect cell expression systems.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.79992-0
2004-06-01
2019-10-14
Loading full text...

Full text loading...

/deliver/fulltext/jgv/85/6/vir851565.html?itemId=/content/journal/jgv/10.1099/vir.0.79992-0&mimeType=html&fmt=ahah

References

  1. Belsham, G. J. & Jackson, R. J. ( 2000; ). Translation initiation on picornavirus RNA. In Translational Control of Gene Expression, 2nd edn, pp. 869–900. Edited by N. Sonenberg, J. W. B. Hershey & M. B. Mathews. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  2. Borman, A. M., Le Mercier, P., Girard, M. & Kean, K. M. ( 1997; ). Comparison of picornaviral IRES-driven internal initiation in cultured cells of different origins. Nucleic Acids Res 25, 925–932.
    [Google Scholar]
  3. Brown, B. A. & Ehrenfeld, E. ( 1979; ). Translation of poliovirus RNA in vitro: changes in cleavage pattern and initiation sites by ribosomal salt wash. Virology 97, 396–405.[CrossRef]
    [Google Scholar]
  4. Carter, M. S., Kuhn, K. M. & Sarnow, P. ( 2000; ). Cellular internal ribosome entry site elements and the use of cDNA microarrays in their investigation. In Translational Control, 2nd edn, pp. 615–634. Edited by J. W. B. Hershey, M. B. Mathews & N. Sonenberg. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  5. Domier, L. L. & McCoppin, N. K. ( 2003; ). In vivo activity of Rhopalosiphum padi virus internal ribosome entry sites. J Gen Virol 84, 415–419.[CrossRef]
    [Google Scholar]
  6. Domier, L. L., McCoppin, N. K. & D'Arcy, C. J. ( 2000; ). Sequence requirements for translation initiation of Rhopalosiphum padi virus ORF2. Virology 268, 264–271.[CrossRef]
    [Google Scholar]
  7. Dorner, A. J., Semler, B. L., Jackson, R. J., Hanecak, R., Duprey, E. & Wimmer, E. ( 1984; ). In vitro translation of poliovirus RNA: utilization of internal initiation sites in reticulocyte lysate. J Virol 50, 507–514.
    [Google Scholar]
  8. Finkelstein, Y., Faktor, O., Elroy-Stein, O. & Levi, B.-Z. ( 1999; ). The use of bi-cistronic transfer vectors for the baculovirus expression system. J Biotechnol 75, 33–44.[CrossRef]
    [Google Scholar]
  9. Glass, M. J. & Summers, D. F. ( 1993; ). Identification of a trans-acting activity from liver that stimulates hepatitis A virus translation in vitro. Virology 193, 1047–1050.[CrossRef]
    [Google Scholar]
  10. Hellen, C. U. T. & Sarnow, P. ( 2001; ). Internal ribosome entry sites in eukaryotic mRNA molecules. Genes Dev 15, 1593–1612.[CrossRef]
    [Google Scholar]
  11. Hershey, J. W. B. & Merrick, W. C. ( 2000; ). Pathway and mechanism of initiation of protein synthesis. In Translational Control, 2nd edn, pp. 33–88. Edited by J. W. B. Hershey, M. B. Mathews & N. Sonenberg. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  12. Kohl, A., Hart, T. J., Noonan, C., Royall, E., Roberts, L. O. & Elliott, R. M. ( 2004; ). A Bunyamwera virus minireplicon system in mosquito cells. J Virol 78 (in press).
    [Google Scholar]
  13. Kubick, S., Schacherl, J., Fleischer-Notter, H., Royall, E., Roberts, L. O. & Stiege, W. ( 2003; ). In vitro translation in an insect-based cell-free system. In Cell-free Protein Expression, pp. 209–217. Edited by J. R. Swartz. Berlin: Springer.
  14. Masoumi, A., Hanzlik, T. N. & Christian, P. D. ( 2003; ). Functionality of the 5′- and intergenic IRES elements of cricket paralysis virus in a range of insect cell lines, and its relationship with viral activities. Virus Res 94, 113–120.[CrossRef]
    [Google Scholar]
  15. Moon, J. S., Domier, L. L., McCoppin, N. K., D'Arcy, C. J. & Jin, H. ( 1998; ). Nucleotide sequence analysis shows that Rhopalosiphum padi virus is a member of a novel group of insect-infecting RNA viruses. Virology 243, 54–65.[CrossRef]
    [Google Scholar]
  16. Nishiyama, T., Yamamoto, H., Shibuya, N., Hatakeyama, Y., Hachimori, A., Uchiumi, T. & Nakashima, N. ( 2003; ). Structural elements in the internal ribosome entry site of Plautia stali intestine virus responsible for binding with ribosomes. Nucleic Acids Res 31, 2434–2442.[CrossRef]
    [Google Scholar]
  17. Roberts, L. O., Seamons, R. A. & Belsham, G. J. ( 1998; ). Recognition of picornavirus internal ribosome entry sites within cells; influence of cellular and viral proteins. RNA 4, 520–529.[CrossRef]
    [Google Scholar]
  18. Sasaki, J. & Nakashima, N. ( 1999; ). Translation initiation at the CUU codon is mediated by the internal ribosome entry site of an insect picorna-like virus in vitro. J Virol 73, 1219–1226.
    [Google Scholar]
  19. van der Velden, A., Kaminski, A., Jackson, R. J. & Belsham, G. J. ( 1995; ). Defective point mutants of the encephalomyocarditis virus internal ribosome entry site can be complemented in trans. Virology 214, 82–90.[CrossRef]
    [Google Scholar]
  20. van Poelwijk, F., Broer, R., Belsham, G. J., Oudshoorn, P., Vlak, J. M. & Goldbach, R. W. ( 1995; ). A hybrid baculovirus-bacteriophage T7 transient expression system. Bio/Tech 13, 261–264.[CrossRef]
    [Google Scholar]
  21. Wilson, J. E., Powell, M. J., Hoover, S. E. & Sarnow, P. ( 2000a; ). Naturally occurring dicistronic cricket paralysis virus RNA is regulated by two internal ribosome entry sites. Mol Cell Biol 20, 4990–4999.[CrossRef]
    [Google Scholar]
  22. Wilson, J. E., Pestova, T. V., Hellen, C. U. T. & Sarnow, P. ( 2000b; ). Initiation from the A site of the ribosome. Cell 102, 511–520.[CrossRef]
    [Google Scholar]
  23. Woolaway, K. E., Lazaridis, K., Belsham, G. J., Carter, M. J. & Roberts, L. O. ( 2001; ). The 5′ UTR of Rhopalosiphum padi virus (RhPV) contains an internal ribosome entry site (IRES) which functions efficiently in mammalian, insect and plant translation systems. J Virol 75, 10244–10249.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.79992-0
Loading
/content/journal/jgv/10.1099/vir.0.79992-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error