1887

Journal of General Virology header logo

Volume 85, Issue 7

Identification and analysis for cross-reactivity among hantaviruses of H-2-restricted cytotoxic T-lymphocyte epitopes in Sin Nombre virus nucleocapsid protein Free

Abstract

Sin Nombre virus (SNV) causes hantavirus pulmonary syndrome (HPS), with a high rate of mortality in humans who are infected by the transmission of virus from the natural rodent host. In humans, cytotoxic T lymphocytes (CTL) specific for SNV appear to play an important role in the pathogenicity of HPS. There is a correlation between the frequencies of SNV-specific CTLs and the severity of HPS disease. In order to create a mouse model to study the role of SNV-specific T cells , T cell responses to SNV nucleocapsid (N) protein in B6.PL /Cy mice (H-2) immunized with plasmid DNA or recombinant vaccinia virus expressing SNV N protein were examined. Four peptides, NC94–101, NC175–189, NC217–231 and NC331–345, were recognized by CD8 T cells in CTL and ELISPOT assays in SNV N-immunized mice. Interestingly, two of these epitopes are located in the central region of the SNV N protein, where several human CD8 T-cell epitopes have been defined in Puumala virus and SNV. CTL lines specific for these four epitopes were cross-reactive to corresponding Puumala virus peptides, but only one of them was cross-reactive to Hantaan virus peptides. These results will enable the analysis of the roles of CTL in immunopathology of HPS in experimental mouse models of HPS.

  • Received:
  • Accepted:
  • Published Online:
SGM
Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.79945-0
2004-07-01
2024-04-16
Loading full text...

Full text loading...

/deliver/fulltext/jgv/85/7/vir851909.html?itemId=/content/journal/jgv/10.1099/vir.0.79945-0&mimeType=html&fmt=ahah

References

  1. Araki K., Yoshimatsu K., Lee B. H., Kariwa H., Takashima I., Arikawa J. 2003; Hantavirus-specific CD8+-T-cell responses in newborn mice persistently infected with Hantaan virus. J Virol 77:8408–8417 [CrossRef]
     [Google Scholar]
  2. Asada H., Tamura M., Kondo K., Dohi Y., Yamanishi K. 1988; Cell-mediated immunity to virus causing haemorrhagic fever with renal syndrome: generation of cytotoxic T lymphocytes. J Gen Virol 69:2179–2188 [CrossRef]
     [Google Scholar]
  3. Asada H., Balachandra K., Tamura M., Kondo K., Yamanishi K. 1989; Cross-reactive immunity among different serotypes of virus causing haemorrhagic fever with renal syndrome. J Gen Virol 70:819–825 [CrossRef]
     [Google Scholar]
  4. Botten J., Mirowsky K., Kusewitt D., Bharadwaj M., Yee J., Ricci R., Feddersen R. M., Hjelle B. 2000; Experimental infection model for Sin Nombre hantavirus in the deer mouse ( Peromyscus maniculatus ). Proc Natl Acad Sci U S A 97:10578–10583 [CrossRef]
     [Google Scholar]
  5. Centers for Disease Control & Prevention. 2004 Case Information: Hantavirus Pulmonary Syndrome Case Count and Descriptive Statistics Atlanta, GA: Centers for Disease Control & Prevention;
     [Google Scholar]
  6. Chu Y. K., Jennings G., Schmaljohn A. 8 other authors 1995; Cross-neutralization of hantaviruses with immune sera from experimentally infected animals and from hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome patients. J Infect Dis 172:1581–1584 [CrossRef]
     [Google Scholar]
  7. de Carvalho Nicacio C., Gonzalez Della Valle M., Padula P., Bjorling E., Plyusnin A., Lundkvist A. 2002; Cross-protection against challenge with Puumala virus after immunization with nucleocapsid proteins from different hantaviruses. J Virol 76:6669–6677 [CrossRef]
     [Google Scholar]
  8. Duchin J. S., Koster F. T., Peters C. J. 13 other authors 1994; Hantavirus pulmonary syndrome: a clinical description of 17 patients with a newly recognized disease. The Hantavirus Study Group. N Engl J Med 330:949–955 [CrossRef]
     [Google Scholar]
  9. Eisenlohr L. C., Yewdell J. W., Bennink J. R. 1992; A transient transfection system for identifying biosynthesized proteins processed and presented to class I MHC restricted T lymphocytes. J Immunol Methods 154:131–138 [CrossRef]
     [Google Scholar]
  10. Enelow R. I., Mohammed A. Z., Stoler M. H., Liu A. N., Young J. S., Lou Y. H., Braciale T. J. 1998; Structural and functional consequences of alveolar cell recognition by CD8+ T lymphocytes in experimental lung disease. J Clin Invest 102:1653–1661 [CrossRef]
     [Google Scholar]
  11. Ennis F. A., Cruz J., Spiropoulou C. F., Waite D., Peters C. J., Nichol S. T., Kariwa H., Koster F. T. 1997; Hantavirus pulmonary syndrome: CD8+ and CD4+ cytotoxic T lymphocytes to epitopes on Sin Nombre virus nucleocapsid protein isolated during acute illness. Virology 238:380–390 [CrossRef]
     [Google Scholar]
  12. Feldmann H., Sanchez A., Morzunov S., Spiropoulou C. F., Rollin P. E., Ksiazek T. G., Peters C. J., Nichol S. T. 1993; Utilization of autopsy RNA for the synthesis of the nucleocapsid antigen of a newly recognized virus associated with hantavirus pulmonary syndrome. Virus Res 30:351–367 [CrossRef]
     [Google Scholar]
  13. Hooper J. W., Larsen T., Custer D. M., Schmaljohn C. S. 2001; A lethal disease model for hantavirus pulmonary syndrome. Virology 289:6–14 [CrossRef]
     [Google Scholar]
  14. Jenison S., Yamada T., Morris C., Anderson B., Torrez-Martinez N., Keller N., Hjelle B. 1994; Characterization of human antibody responses to four corners hantavirus infections among patients with hantavirus pulmonary syndrome. J Virol 68:3000–3006
     [Google Scholar]
  15. Kanerva M., Mustonen J., Vaheri A. 1998; Pathogenesis of puumala and other hantavirus infections. Rev Med Virol 8:67–86 [CrossRef]
     [Google Scholar]
  16. Kilpatrick E. D., Terajima M., Koster F. T., Catalina M. D., Cruz J., Ennis F. A. 2004; Role of specific CD8+ T cells in the severity of a fulminant zoonotic viral hemorrhagic fever, hantavirus pulmonary syndrome. J Immunol 172:3297–3304 [CrossRef]
     [Google Scholar]
  17. Koster F. T., Williams T. M., Griffith B. B., Goade D. E., Hjelle B. L. 1998; Genetic associations with hantavirus pulmonary syndrome due to Sin Nombre virus. In The Fourth International Conference on HFRS and Hantaviruses p– 179 Atlanta, GA, USA:
     [Google Scholar]
  18. Kurane I., Ennis F. A. 1994; Cytokines in dengue virus infections: role of cytokines in the pathogenesis of dengue hemorrhagic fever. Semin Virol 5:443–448 [CrossRef]
     [Google Scholar]
  19. Lee K. Y., Chun E., Kim N. Y., Seong B. L. 2002; Characterization of HLA-A2.1-restricted epitopes, conserved in both Hantaan and Sin Nombre viruses, in Hantaan virus-infected patients. J Gen Virol 83:1131–1136
     [Google Scholar]
  20. Milazzo M. L., Eyzaguirre E. J., Molina C. P., Fulhorst C. F. 2002; Maporal viral infection in the Syrian golden hamster: a model of hantavirus pulmonary syndrome. J Infect Dis 186:1390–1395 [CrossRef]
     [Google Scholar]
  21. Nakamura T., Yanagihara R., Gibbs C. J. Jr, Amyx H. L., Gajdusek D. C. 1985a; Differential susceptibility and resistance of immunocompetent and immunodeficient mice to fatal Hantaan virus infection. Arch Virol 86:109–120 [CrossRef]
     [Google Scholar]
  22. Nakamura T., Yanagihara R., Gibbs C. J. Jr, Gajdusek D. C. 1985b; Immune spleen cell-mediated protection against fatal Hantaan virus infection in infant mice. J Infect Dis 151:691–697 [CrossRef]
     [Google Scholar]
  23. Nolte K. B., Feddersen R. M., Foucar K. 7 other authors 1995; Hantavirus pulmonary syndrome in the United States: a pathological description of a disease caused by a new agent. Hum Pathol 26:110–120 [CrossRef]
     [Google Scholar]
  24. Park J. M., Cho S. Y., Hwang Y. K., Um S. H., Kim W. J., Cheong H. S., Byun S. M. 2000; Identification of H-2Kb-restricted T-cell epitopes within the nucleocapsid protein of Hantaan virus and establishment of cytotoxic T-cell clones. J Med Virol 60:189–199 [CrossRef]
     [Google Scholar]
  25. Parker K. C., Bednarek M. A., Coligan J. E. 1994; Scheme for ranking potential HLA-A2 binding peptides based on independent binding of individual peptide side-chains. J Immunol 152:163–175
     [Google Scholar]
  26. Pearson A., Richardson C., Yuen L. 1991; The 5′ noncoding region sequence of the Choristoneura biennis entomopoxvirus spheroidin gene functions as an efficient late promoter in the mammalian vaccinia expression system. Virology 180:561–566 [CrossRef]
     [Google Scholar]
  27. Rammensee H.-G., Friede T., Stevanovic S. 1995; MHC ligands and peptide motifs: first listing. Immunogenetics 41:178–228 [CrossRef]
     [Google Scholar]
  28. Rammensee H.-G., Bachmann J., Emmerich N. P. N., Bachor O. A., Stevanovic S. 1999; syfpeithi: database for MHC ligands and peptide motifs. Immunogenetics 50:213–219 [CrossRef]
     [Google Scholar]
  29. Rothman A. L., Ennis F. A. 1999; Immunopathogenesis of Dengue hemorrhagic fever. Virology 257:1–6 [CrossRef]
     [Google Scholar]
  30. Ruo S. L., Sanchez A., Elliott L. H., Brammer L. S., McCormick J. B., Fisher-Hoch S. P. 1991; Monoclonal antibodies to three strains of hantaviruses: Hantaan, R22, and Puumala. Arch Virol 119:1–11 [CrossRef]
     [Google Scholar]
  31. Schmaljohn C., Hjelle B. 1997; Hantaviruses: a global disease problem. Emerg Infect Dis 3:95–104 [CrossRef]
     [Google Scholar]
  32. Schmaljohn C. S., Chu Y. K., Schmaljohn A. L., Dalrymple J. M. 1990; Antigenic subunits of Hantaan virus expressed by baculovirus and vaccinia virus recombinants. J Virol 64:3162–3170
     [Google Scholar]
  33. Terajima M., Hendershot J. D. III, Kariwa H., Koster F. T., Hjelle B., Goade D., DeFronzo M. C., Ennis F. A. 1999; High levels of viremia in patients with the Hantavirus pulmonary syndrome. J Infect Dis 180:2030–2034 [CrossRef]
     [Google Scholar]
  34. Terajima M., Van Epps H. L., Li D., Leporati A. M., Juhlin S. E., Mustonen J., Vaheri A., Ennis F. A. 2002; Generation of recombinant vaccinia viruses expressing Puumala virus proteins and use in isolating cytotoxic T cells specific for Puumala virus. Virus Res 84:67–77 [CrossRef]
     [Google Scholar]
  35. Terajima M., Vapalahti O., Epps H. L. V., Vaheri A., Ennis F. A. 2004; Immune responses to Puumala virus infection and the pathogenesis of nephropathia epidemica. Microbes Infect 6:238–245 [CrossRef]
     [Google Scholar]
  36. Van Epps H. L., Schmaljohn C. S., Ennis F. A. 1999; Human memory cytotoxic T-lymphocyte (CTL) responses to Hantaan virus infection: identification of virus-specific and cross-reactive CD8+ CTL epitopes on nucleocapsid protein. J Virol 73:5301–5308
     [Google Scholar]
  37. Van Epps H. L., Terajima M., Mustonen J., Arstila T. P., Corey E. A., Vaheri A., Ennis F. A. 2002; Long-lived memory T lymphocyte responses after hantavirus infection. J Exp Med 196:579–588 [CrossRef]
     [Google Scholar]
  38. Zaki S. R., Greer P. W., Coffield L. M. 7 other authors 1995; Hantavirus pulmonary syndrome. Pathogenesis of an emerging infectious disease. Am J Pathol 146:552–579
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.79945-0
Loading
Identification and analysis for cross-reactivity among hantaviruses of H-2b-restricted cytotoxic T-lymphocyte epitopes in Sin Nombre virus nucleocapsid protein
J. Gen. Virol. 85, 1909 (2004); https://doi.org/10.1099/vir.0.79945-0
/content/journal/jgv/10.1099/vir.0.79945-0
/content/journal/jgv/10.1099/vir.0.79945-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error