1887

Abstract

To evaluate RNA silencing for the control of geminivirus infection, two classes of post-transcriptionally silenced (PTS) plants were tested using (TYLCSV) Rep-210-transgenic plants, a sense×antisense hybrid and two multicopy sense lines. In both classes, PTS plants accumulated low or undetectable amounts of Rep-210 protein and mRNA but high amounts of Rep-210 small interfering RNAs. PTS plants were susceptible to TYLCSV when challenged by agroinoculation or using high viruliferous whitefly () pressure, although some plants were resistant at low whitefly pressure. Delayed infections were also observed, indicating that TYLCSV could overcome transgene silencing of and of the nested gene. TYLCSV infection boosted transgene silencing but this did not lead to recovery. The data suggest that if the virus reaches a threshold level of expression/replication in the initially infected cells then virus spreading can no longer be prevented.

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2004-06-01
2019-12-11
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References

  1. Bass, B. L. ( 2000; ). Double-stranded RNA as a template for gene silencing. Cell 101, 235–238.[CrossRef]
    [Google Scholar]
  2. Baulcombe, D. C. ( 1996; ). Mechanisms of pathogen-derived resistance to viruses in transgenic plants. Plant Cell 8, 1833–1844.[CrossRef]
    [Google Scholar]
  3. Bernstein, E., Caudy, A. A., Hammond, S. M. & Hannon, G. J. ( 2001; ). Role for a bidentate ribonuclease in the initiation step of RNA interference. Nature 409, 363–366.[CrossRef]
    [Google Scholar]
  4. Brunetti, A., Tavazza, M., Noris, E., Tavazza, R., Caciagli, P., Ancora, G., Crespi, S. & Accotto, G. P. ( 1997; ). High expression of truncated viral Rep protein confers resistance to tomato yellow leaf curl virus in transgenic tomato plants. Mol Plant Microbe Interact 10, 571–579.[CrossRef]
    [Google Scholar]
  5. Brunetti, A., Tavazza, R., Noris, E., Lucioli, A., Accotto, G. P. & Tavazza, M. ( 2001; ). Transgenically expressed T-Rep of tomato yellow leaf curl Sardinia virus acts as a trans-dominant-negative mutant, inhibiting viral transcription and replication. J Virol 75, 10573–10581.[CrossRef]
    [Google Scholar]
  6. Dong, X., van Wezel, R., Stanley, J. & Hong, Y. ( 2003; ). Functional characterization of the nuclear localization signal for a suppressor of posttranscriptional gene silencing. J Virol 77, 7026–7033.[CrossRef]
    [Google Scholar]
  7. Elbashir, S. M., Harborth, J., Lendeckel, W., Yalcin, A., Weber, K. & Tuschl, T. ( 2001a; ). Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 411, 494–498.[CrossRef]
    [Google Scholar]
  8. Elbashir, S. M., Lendeckel, W. & Tuschl, T. ( 2001b; ). RNA interference is mediated by 21- and 22-nucleotide RNAs. Genes Dev 15, 188–200.[CrossRef]
    [Google Scholar]
  9. Goodwin, J., Chapman, K., Swaney, S., Parks, T. D., Wernsman, E. A. & Dougherty, W. G. ( 1996; ). Genetic and biochemical dissection of transgenic RNA-mediated virus resistance. Plant Cell 8, 95–105.[CrossRef]
    [Google Scholar]
  10. Guerineau, F. & Mullineaux, P. ( 1993; ). Plant transformation and expression vectors. In Plant Molecular Biology Labfax, pp. 121–147. Edited by R. R. D. Croy. Oxford, UK: BIOS Scientific Publishers.
  11. Guo, H. S. & Ding, S. W. ( 2002; ). A viral protein inhibits the long range signaling activity of the gene silencing signal. EMBO J 21, 398–407.[CrossRef]
    [Google Scholar]
  12. Hamilton, A. J. & Baulcombe, D. C. ( 1999; ). A species of small antisense RNA in posttranscriptional gene silencing in plants. Science 286, 950–952.[CrossRef]
    [Google Scholar]
  13. Hamilton, A., Voinnet, O., Chappell, L. & Baulcombe, D. ( 2002; ). Two classes of short interfering RNA in RNA silencing. EMBO J 21, 4671–4679.[CrossRef]
    [Google Scholar]
  14. Hammond, S. M., Bernstein, E., Beach, D. & Hannon, G. J. ( 2000; ). An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells. Nature 404, 293–296.[CrossRef]
    [Google Scholar]
  15. Jupin, I., De Kouchkovsky, F., Jouanneau, F. & Gronenborn, B. ( 1994; ). Movement of tomato yellow leaf curl geminivirus (TYLCV): involvement of the protein encoded by ORF C4. Virology 204, 82–90.[CrossRef]
    [Google Scholar]
  16. Kumagai, M. H., Donson, J., della-Cioppa, G., Harvey, D., Hanley, K. & Grill, L. K. ( 1995; ). Cytoplasmic inhibition of carotenoid biosynthesis with virus-derived RNA. Proc Natl Acad Sci U S A 92, 1679–1683.[CrossRef]
    [Google Scholar]
  17. Laufs, J., Jupin, I., David, C., Schumacher, S., Heyraud-Nitschke, F. & Gronenborn, B. ( 1995; ). Geminivirus replication: genetic and biochemical characterization of Rep protein function, a review. Biochimie 77, 765–773.[CrossRef]
    [Google Scholar]
  18. Llave, C., Kasschau, K. D. & Carrington, J. C. ( 2000; ). Virus-encoded suppressor of posttranscriptional gene silencing targets a maintenance step in the silencing pathway. Proc Natl Acad Sci U S A 97, 13401–13406.[CrossRef]
    [Google Scholar]
  19. Lucioli, A., Noris, E., Brunetti, A., Tavazza, R., Ruzza, V., Castillo, A. G., Bejarano, E. R., Accotto, G. P. & Tavazza, M. ( 2003; ). Tomato yellow leaf curl Sardinia virus rep-derived resistance to homologous and heterologous geminiviruses occurs by different mechanisms and is overcome if virus-mediated transgene silencing is activated. J Virol 77, 6785–6798.[CrossRef]
    [Google Scholar]
  20. Mallory, A. C., Ely, L., Smith, T. H. & 7 other authors ( 2001; ). HC-Pro suppression of transgene silencing eliminates the small RNAs but not transgene methylation or the mobile signal. Plant Cell 13, 571–583.[CrossRef]
    [Google Scholar]
  21. Noris, E., Accotto, G. P., Tavazza, R., Brunetti, A., Crespi, S. & Tavazza, M. ( 1996; ). Resistance to tomato yellow leaf curl geminivirus in Nicotiana benthamiana plants transformed with a truncated viral C1 gene. Virology 224, 130–138.[CrossRef]
    [Google Scholar]
  22. Pooggin, M., Shivaprasad, P. V., Veluthambi, K. & Hohn, T. ( 2003; ). RNAi targeting of DNA virus in plants. Nat Biotechnol 21, 131–132.
    [Google Scholar]
  23. Rojas, M. R., Jiang, H., Salati, R., Xoconostle-Cazares, B., Sudarshana, M. R., Lucas, W. J. & Gilbertson, R. L. ( 2001; ). Functional analysis of proteins involved in movement of the monopartite begomovirus, tomato yellow leaf curl virus. Virology 291, 110–125.[CrossRef]
    [Google Scholar]
  24. Ruiz, M. T., Voinnet, O. & Baulcombe, D. C. ( 1998; ). Initiation and maintenance of virus-induced gene silencing. Plant Cell 10, 937–946.[CrossRef]
    [Google Scholar]
  25. Silhavy, D., Molnar, A., Lucioli, A., Szittya, G., Hornyik, C., Tavazza, M. & Burgyan, J. ( 2002; ). A viral protein suppresses RNA silencing and binds silencing-generated, 21- to 25-nucleotide double-stranded RNAs. EMBO J 21, 3070–3080.[CrossRef]
    [Google Scholar]
  26. Stam, M., de Bruin, R., Kenter, S., van der Hoorn, R. A. L., van Blokland, R., Mol, J. N. M. & Kooter, J. M. ( 1997; ). Post-transcriptional silencing of chalcone synthase in Petunia by inverted transgene repeats. Plant J 12, 63–82.[CrossRef]
    [Google Scholar]
  27. Tang, G., Reinhart, B. J., Bartel, D. P. & Zamore, P. D. ( 2003; ). A biochemical framework for RNA silencing in plants. Genes Dev 17, 49–63.[CrossRef]
    [Google Scholar]
  28. Vanitharani, R., Chellappan, P. & Fauquet, C. M. ( 2003; ). Short interfering RNA-mediated interference of gene expression and viral DNA accumulation in cultured plant cells. Proc Natl Acad Sci U S A 100, 9632–9636.[CrossRef]
    [Google Scholar]
  29. van Wezel, R., Dong, X., Liu, H., Tien, P., Stanley, J. & Hong, Y. ( 2002; ). Mutation of three cysteine residues in tomato yellow leaf curl virus-China C2 protein causes dysfunction in pathogenesis and posttranscriptional gene-silencing suppression. Mol Plant Microbe Interact 15, 203–208.[CrossRef]
    [Google Scholar]
  30. Voinnet, O. ( 2001; ). RNA silencing as a plant immune system against viruses. Trends Genet 17, 449–459.[CrossRef]
    [Google Scholar]
  31. Voinnet, O., Pinto, Y. M. & Baulcombe, D. C. ( 1999; ). Suppression of gene silencing: a general strategy used by diverse DNA and RNA viruses of plants. Proc Natl Acad Sci U S A 96, 14147–14152.[CrossRef]
    [Google Scholar]
  32. Voinnet, O., Lederer, C. & Baulcombe, D. C. ( 2000; ). A viral movement protein prevents spread of the gene silencing signal in Nicotiana benthamiana. Cell 103, 157–167.[CrossRef]
    [Google Scholar]
  33. Waterhouse, P. M., Graham, M. W. & Wang, M. B. ( 1998; ). Virus resistance and gene silencing in plants can be induced by simultaneous expression of sense and antisense RNA. Proc Natl Acad Sci U S A 95, 13959–13964.[CrossRef]
    [Google Scholar]
  34. Waterhouse, P. M., Wang, M. B. & Lough, T. ( 2001; ). Gene silencing as an adaptive defence against viruses. Nature 411, 834–842.[CrossRef]
    [Google Scholar]
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