1887

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Volume 85, Issue 6

can overcome transgene-mediated RNA silencing of two essential viral genes Free

Abstract

To evaluate RNA silencing for the control of geminivirus infection, two classes of post-transcriptionally silenced (PTS) plants were tested using (TYLCSV) Rep-210-transgenic plants, a sense×antisense hybrid and two multicopy sense lines. In both classes, PTS plants accumulated low or undetectable amounts of Rep-210 protein and mRNA but high amounts of Rep-210 small interfering RNAs. PTS plants were susceptible to TYLCSV when challenged by agroinoculation or using high viruliferous whitefly () pressure, although some plants were resistant at low whitefly pressure. Delayed infections were also observed, indicating that TYLCSV could overcome transgene silencing of and of the nested gene. TYLCSV infection boosted transgene silencing but this did not lead to recovery. The data suggest that if the virus reaches a threshold level of expression/replication in the initially infected cells then virus spreading can no longer be prevented.

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2004-06-01
2024-04-19
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Tomato yellow leaf curl Sardinia virus can overcome transgene-mediated RNA silencing of two essential viral genes
J. Gen. Virol. 85, 1745 (2004); https://doi.org/10.1099/vir.0.79944-0
/content/journal/jgv/10.1099/vir.0.79944-0
/content/journal/jgv/10.1099/vir.0.79944-0
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