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1887

Journal of General Virology header logo Journal of General Virology

Volume 85, Issue 7

Inhibition of influenza virus matrix (M1) protein expression and virus replication by U6 promoter-driven and lentivirus-mediated delivery of siRNA No Access

Abstract

Small interfering RNA (siRNA)-induced RNA degradation has been used recently as an antivirus agent to inhibit specific virus replication. This report shows that 21 nt duplexes of siRNA of the influenza virus M gene can cause specific inhibition of influenza virus matrix (M1) protein expression in transfected 293T cells. Furthermore, it is shown that a lentivirus vector can be used to effectively deliver M gene siRNAs into Madin–Darby canine kidney cells and can cause specific inhibition of M1 protein expression and influenza virus replication. Therefore, lentivirus-mediated delivery of siRNA and gene silencing can be used in studying the specific functions of virus genes in replication and may have a potential therapeutic application.

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/content/journal/jgv/10.1099/vir.0.79906-0
2004-07-01
2025-02-08
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/content/journal/jgv/10.1099/vir.0.79906-0
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Inhibition of influenza virus matrix (M1) protein expression and virus replication by U6 promoter-driven and lentivirus-mediated delivery of siRNA
J. Gen. Virol. 85, 1877 (2004); https://doi.org/10.1099/vir.0.79906-0
/content/journal/jgv/10.1099/vir.0.79906-0
/content/journal/jgv/10.1099/vir.0.79906-0
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