Sequences of flavivirus-related RNA viruses persist in DNA form integrated in the genome of spp. mosquitoes Free

Abstract

Flavivirus-related sequences have been discovered in the dsDNA genome of and mosquitoes, demonstrating for the first time an integration into a eukaryotic genome of a multigenic sequence from an RNA virus that replicates without a recognized DNA intermediate. In the C6/36 cell line, an open reading frame (ORF) of 1557 aa with protease/helicase and polyprotein processing domains characteristic of flaviviruses was identified. It is closely related to NS1–NS4A genes of the and and the corresponding mRNAs were detected. Integrated sequences homologous to the envelope, NS4B and polymerase genes of flaviviruses were identified. Overall, approximately two-thirds of a flavivirus-like genome were characterized. In the A20 cell line, a 492 aa ORF related to the polymerase of the and was identified. These flavivirus-related integrated DNA sequences were detected in laboratory-bred and wild and mosquitoes, demonstrating that their discovery is not an artefact resulting from the manipulation of mosquito cell lines, since they exist under natural conditions. This finding has major implications regarding evolution, as it represents an entirely different mechanism by which genetic diversity may be generated in eukaryotic cells distinct from accepted processes.

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2004-07-01
2024-03-28
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