1887

Abstract

Flavivirus-related sequences have been discovered in the dsDNA genome of and mosquitoes, demonstrating for the first time an integration into a eukaryotic genome of a multigenic sequence from an RNA virus that replicates without a recognized DNA intermediate. In the C6/36 cell line, an open reading frame (ORF) of 1557 aa with protease/helicase and polyprotein processing domains characteristic of flaviviruses was identified. It is closely related to NS1–NS4A genes of the and and the corresponding mRNAs were detected. Integrated sequences homologous to the envelope, NS4B and polymerase genes of flaviviruses were identified. Overall, approximately two-thirds of a flavivirus-like genome were characterized. In the A20 cell line, a 492 aa ORF related to the polymerase of the and was identified. These flavivirus-related integrated DNA sequences were detected in laboratory-bred and wild and mosquitoes, demonstrating that their discovery is not an artefact resulting from the manipulation of mosquito cell lines, since they exist under natural conditions. This finding has major implications regarding evolution, as it represents an entirely different mechanism by which genetic diversity may be generated in eukaryotic cells distinct from accepted processes.

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2004-07-01
2019-11-19
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References

  1. Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J. ( 1990; ). Basic local alignment search tool. J Mol Biol 215, 403–410.[CrossRef]
    [Google Scholar]
  2. Cammisa-Parks, H., Cisar, L. A., Kane, A. & Stollar, V. ( 1992; ). The complete nucleotide sequence of cell fusing agent (CFA): homology between the nonstructural proteins encoded by CFA and the nonstructural proteins encoded by arthropod-borne flaviviruses. Virology 189, 511–524.[CrossRef]
    [Google Scholar]
  3. Charrel, R. N., De Micco, P. & de Lamballerie, X. ( 1999; ). Phylogenetic analysis of GB viruses A and C: evidence for cospeciation between virus isolates and their primate hosts. J Gen Virol 80, 2329–2335.
    [Google Scholar]
  4. Crabtree, M. B., Sang, R. C., Stollar, V., Dunster, L. M. & Miller, B. R. ( 2003; ). Genetic and phenotypic characterization of the newly described insect flavivirus, Kamiti River virus. Arch Virol 148, 1095–1118.[CrossRef]
    [Google Scholar]
  5. de Lamballerie, X., Crochu, S., Billoir, F., Neyts, J., de Micco, P., Holmes, E. C. & Gould, E. A. ( 2002; ). Genome sequence analysis of Tamana bat virus and its relationship with the genus Flavivirus. J Gen Virol 83, 2443–2454.
    [Google Scholar]
  6. Drynov, I. D., Uryvaev, L. V., Nosikov, V. V. & Zhdanov, V. M. ( 1981; ). Integration of the genomes of the tick-borne encephalitis virus and of the cell in chronic infection due to this virus and SV40. Dokl Akad Nauk SSSR 258, 1000–1002 (in Russian).
    [Google Scholar]
  7. Gaidamovich, S. Y., Cherednichenko, Y. N. & Zhdanov, V. M. ( 1978; ). On the mechanism of the persistence of lymphocytic choriomeningitis virus in the continuous cell line Detroit-6. Intervirology 9, 156–161.[CrossRef]
    [Google Scholar]
  8. Gaunt, M. W. & Miles, A. ( 2002; ). An insect molecular clock dates the origin of the insects and accords with palaeontological and biogeographic landmarks. Mol Biol Evol 19, 748–761.[CrossRef]
    [Google Scholar]
  9. Igarashi, A. ( 1978; ). Isolation of a Singh's Aedes albopictus cell clone sensitive to Dengue and Chikungunya viruses. J Gen Virol 40, 531–544.[CrossRef]
    [Google Scholar]
  10. Klenerman, P., Hengartner, H. & Zinkernagel, R. M. ( 1997; ). A non-retroviral RNA virus persists in DNA form. Nature 390, 298–301.[CrossRef]
    [Google Scholar]
  11. Kumar, S., Tamura, K., Jakobsen, I. B. & Nei, M. ( 2001; ). mega2: molecular evolutionary genetics analysis software. Bioinformatics 17, 1244–1245.[CrossRef]
    [Google Scholar]
  12. Kyte, J. & Doolittle, R. F. ( 1982; ). A simple method for displaying the hydropathic character of a protein. J Mol Biol 157, 105–132.[CrossRef]
    [Google Scholar]
  13. Malik, H. S., Henikoff, S. & Eickbush, T. H. ( 2000; ). Poised for contagion: evolutionary origins of the infectious abilities of invertebrate retroviruses. Genome Res 10, 1307–1318.[CrossRef]
    [Google Scholar]
  14. Meyerhans, A. & Vartanian, J.-P. ( 1999; ). The fidelity of cellular and viral polymerases and its manipulation for hypermutagenesis. In Origin and Evolution of Viruses, pp. 87–114. Edited by E. Domingo, R. Webster & J. Holland. London: Academic Press.
  15. Morvan, J. M., Deubel, V., Gounon, P. & 9 other authors ( 1999; ). Identification of Ebola virus sequences present as RNA or DNA in organs of terrestrial small mammals of the Central African Republic. Microbes Infect 1, 1193–1201.[CrossRef]
    [Google Scholar]
  16. Sambrook, J., Fritsch, E. F. & Maniatis, T. ( 1989; ). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  17. Sang, R. C., Gichogo, A., Gachoya, J., Dunster, M. D., Ofula, V., Hunt, A. R., Crabtree, M. B., Miller, B. R. & Dunster, L. M. ( 2003; ). Isolation of a new flavivirus related to cell fusing agent virus (CFAV) from field-collected flood-water Aedes mosquitoes sampled from a dambo in central Kenya. Arch Virol 148, 1085–1093.[CrossRef]
    [Google Scholar]
  18. Shouche, Y. S. & Patole, M. S. ( 2000; ). Sequence analysis of mitochondrial 16S ribosomal RNA gene fragment from seven mosquito species. J Biosci 25, 361–366.[CrossRef]
    [Google Scholar]
  19. Simmons, R. B. & Weller, S. J. ( 2001; ). Utility and evolution of cytochrome b in insects. Mol Phylogenet Evol 20, 196–210.[CrossRef]
    [Google Scholar]
  20. Singh, K. R. & Bhat, U. K. ( 1971; ). Establishment of 2 mosquito cell lines from larval tissues of Aedes w-albus. Experientia 27, 142–143.[CrossRef]
    [Google Scholar]
  21. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  22. Twiddy, S. S., Holmes, E. C. & Rambaut, A. ( 2003; ). Inferring the rate and time-scale of dengue virus evolution. Mol Biol Evol 20, 122–129.[CrossRef]
    [Google Scholar]
  23. Zhdanov, V. M. ( 1975; ). Integration of viral genomes. Nature 256, 471–473.[CrossRef]
    [Google Scholar]
  24. Zhdanov, V. M. & Azadova, N. B. ( 1976; ). Integration and transfection of an arbovirus by mammalian cells. Mol Biol (Mosk) 10, 1296–1302 (in Russian).
    [Google Scholar]
  25. Zhdanov, V. M. & Parfanovich, M. I. ( 1974; ). Integration of measles virus nucleic acid into the cell genome. Arch Gesamte Virusforsch 45, 225–234.[CrossRef]
    [Google Scholar]
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