1887

Abstract

Here is described the isolation of a naturally occurring A-type inclusion body (ATI)-negative vaccinia-like virus, (VBH), obtained from a mousepox-like outbreak in Brazil. The isolated virus was identified and characterized as an orthopoxvirus by conventional methods. Molecular characterization of the virus was done by DNA cross-hybridization using (VACV) DNA. In addition, conserved orthopoxvirus genes such as vaccinia growth factor, thymidine kinase and haemagglutinin were amplified by PCR and sequenced. All sequences presented high similarity to VACV genes. Based on the sequences, phenograms were constructed for comparison with other poxviruses; VBH clustered consistently with VACV strains. Attempts to amplify the ATI gene () by PCR, currently used to identify orthopoxviruses, were unsuccessful. Results presented here suggest that most of the gene is deleted in the VBH genome.

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2004-07-01
2024-04-25
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References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410 [CrossRef]
    [Google Scholar]
  2. Buller R. M. L., Palumbo G. J. 1991; Poxvirus pathogenesis. Microbiol Rev 55:80–122
    [Google Scholar]
  3. Campos M. A. S., Kroon E. G. 1993; Critical period for reversible block of vaccinia virus replication. Rev Braz Microbiol 24:104–110
    [Google Scholar]
  4. Chantrey J., Meyer H., Baxby D., Begon M., Bown K. J., Hazel S. M., Jones T., Montgomery W. I., Bennett M. 1999; Cowpox: reservoir hosts and geographic range. Epidemiol Infect 122:455–460 [CrossRef]
    [Google Scholar]
  5. Church G. M., Gilbert W. 1984; Genomic sequencing. Proc Natl Acad Sci U S A 81:1991–1995 [CrossRef]
    [Google Scholar]
  6. da Fonseca F. G., Lanna M. C. S., Campos M. A. S., Kitajima E. W., Peres J. N., Golgher R. R., Ferreira P. C. P., Kroon E. G. 1998; Morphological and molecular characterization of the poxvirus BeAn 58058. Arch Virol 143:1171–1186 [CrossRef]
    [Google Scholar]
  7. da Fonseca F. G., Silva R. L. A., Marques J. T., Ferreira P. C. P., Kroon E. G. 1999; The genome of cowpox virus contains a gene related to those encoding the epidermal growth factor, transforming growth factor alpha and vaccinia growth factor. Virus Genes 18:151–160 [CrossRef]
    [Google Scholar]
  8. da Fonseca F. G., Trindade G. S., Silva R. L. A., Bonjardim C. A., Ferreira P. C. P., Kroon E. G. 2002; Characterization of a vaccinia-like virus isolated in a Brazilian forest. J Gen Virol 83:223–228
    [Google Scholar]
  9. Damaso C. R. A., Esposito J. J., Condit R., Moussatché N. 2000; An emergent poxvirus from humans and cattle in Rio de Janeiro state: Cantagalo virus may derive from Brazilian smallpox vaccine. Virology 277:439–449 [CrossRef]
    [Google Scholar]
  10. Diniz S., Trindade G. S., da Fonseca F. G., Kroon E. G. 2001; An outbreak of mousepox in Swiss mice in a laboratory animal facility – case report. Arq Braz Med Vet Zootec 53:152–156
    [Google Scholar]
  11. Esposito J. J., Knight J. C. 1985; Orthopoxvirus DNA: a comparison of restriction profiles and maps. Virology 143:230–251 [CrossRef]
    [Google Scholar]
  12. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  13. Fenner F., Wittek R., Dumbell K. R. 1989; The global spread, control and eradication of smallpox. In The Orthopoxviruses pp  317–352 San Diego, CA: Academic Press;
    [Google Scholar]
  14. Funahashi S., Sato T., Shida H. 1988; Cloning and characterization of the gene encoding the major protein of the A-type inclusion body of cowpox virus. J Gen Virol 69:35–47 [CrossRef]
    [Google Scholar]
  15. Goebel S. J., Johson G. P., Perkus M. E., Davis S. W., Winslow J. P., Paoletti E. 1990; The complete DNA sequence of vaccinia virus. Virology 179:247–266 [CrossRef]
    [Google Scholar]
  16. Hall T. A. 1999; bioedit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
    [Google Scholar]
  17. Hasegawa M., Kishino H., Yano T. 1985; Dating of the human–ape splitting by a molecular clock of mitochondrial DNA. J Mol Evol 22:160–174 [CrossRef]
    [Google Scholar]
  18. Hazel S. M., Bennett M., Chantrey J., Bown K., Cavanagh R., Jones T. R., Baxby D., Begon M. A. 2000; A longitudinal study of an endemic disease in its wildlife reservoir: cowpox and wild rodents. Epidemiol Infect 124:551–562 [CrossRef]
    [Google Scholar]
  19. Johnson G. P., Goebel S. J., Paoletti E. 1993; An update on the vaccinia virus genome. Virology 196:381–401 [CrossRef]
    [Google Scholar]
  20. Joklik W. K. 1962; The purification of four strains of poxvirus. Virology 18:9–18 [CrossRef]
    [Google Scholar]
  21. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism pp  21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  22. Marques J. T., Trindade G. S., da Fonseca F. G., dos Santos J. R., Bonjardim C. A., Ferreira P. C. P., Kroon E. G. 2001; Characterization of ATI, TK and IFN-alpha/betaR genes in the genome of the BeAn 58058 virus, a naturally attenuated wild orthopoxvirus. Virus Genes 23:291–301 [CrossRef]
    [Google Scholar]
  23. Massung R. F., Moyer R. W. 1991; The molecular biology of swinepox virus. I. A characterization of the viral DNA. Virology 180:347–354 [CrossRef]
    [Google Scholar]
  24. Meyer H., Pfeffer M., Rziha H. J. 1994; Sequence alterations within and downstream of the A-type inclusion protein genes allow differentiation of othopoxvirus species by polymerase chain reaction. J Gen Virol 75:1975–1981 [CrossRef]
    [Google Scholar]
  25. Meyer H., Roop S. L., Esposito J. J. 1997; Gene for A-type inclusion body protein is useful for a polymerase chain reaction assay to differentiate orthopoxvirus. J Virol Methods 64:217–221 [CrossRef]
    [Google Scholar]
  26. Neubauer H., Pfeffer M., Meyer H. 1997; Specific detection of mousepox virus by polymerase chain reaction. Lab Anim 31:201–205 [CrossRef]
    [Google Scholar]
  27. Osterrieder N., Meyer H., Pfeffer M. 1994; Characterization of the gene encoding the A-type inclusion body protein of mousepox virus. Virus Genes 8:125–135 [CrossRef]
    [Google Scholar]
  28. Raes J., Van der Peer Y. 1999; forcon, a tool to automatically convert sequence alignment formats. EMBnet.news 6 (1): http://www.psb.rug.ac.be/∼jerae/ForCon/index.html
    [Google Scholar]
  29. Ronquist F., Huelsenbeck J. P. 2003; mrbayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574 [CrossRef]
    [Google Scholar]
  30. Roop S. L., Jin Q. I., Knight J. C., Massung R. F., Esposito J. J. 1995; PCR strategy for identification and differentiation of smallpox and other orthopoxviruses. J Clin Microbiol 33:2069–2076
    [Google Scholar]
  31. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  32. Sambrook J., Fritsch E. F., Maniatis T. 1989; Analysis and cloning of eukaryotic genomic DNA. In Molecular Cloning: A Laboratory Manual . , 2nd edn. vol 2pp. 9.1–9.62 Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
  33. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A 74:5463–5467 [CrossRef]
    [Google Scholar]
  34. Strimmer K., von Haeseler A. 1996; Quartet puzzling: a quartet maximum likelihood method for reconstructing tree topologies. Mol Biol Evol 13:964–969 [CrossRef]
    [Google Scholar]
  35. Swofford D. L. 1998 paup*. Phylogenetic Analysis Using Parsimony (*and Other Methods) . Version 4 Sunderland, MA: Sinauer Associates;
    [Google Scholar]
  36. Tajima F., Nei M. 1984; Estimation of evolutionary distance between nucleotide sequences. Mol Biol Evol 1:269–285
    [Google Scholar]
  37. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  38. Trindade G. S., Fonseca F. G., Marques J. T. 8 other authors 2003; Araçatuba virus: a vaccinia-like virus associated with cattle and human infection. Emerg Infect Dis 9:155–160 [CrossRef]
    [Google Scholar]
  39. Van de Peer Y., de Wachter R. 1997; Construction of evolutionary distance trees with treecon for Windows: accounting for variation in nucleotide substitution rate among sites. Comput Appl Biosci 13:227–230
    [Google Scholar]
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