1887

Abstract

Previously, it has been shown that tobacco mosaic virus (TMV) U1 and crucifer-infecting TMV contain a 75 nt internal ribosome entry site (IRES) upstream of movement protein (MP) gene (IRES and IRES , respectively). A movement-deficient TMV mutant, KK6, has been constructed previously [ Lehto, K., Grantham, G. L. & Dawson, W. O. (1990). , 145–157 ] by insertion of the second coat protein subgenomic promoter (CP SGP-2) upstream of the MP gene, in addition to the natural CP SGP-1. Here, the authors compare the efficiency of movement function expression by KK6 and a derivative, K86, obtained by insertion of IRES between the CP SGP-2 and MP genes resulting in restoration of IRES function in the 5′-untranslated sequence of the I subgenomic RNA of K86. The data indicate that the efficiency of K86 movement was largely restored by this insertion, which was apparently due to the translation-enhancing ability of IRES .

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.79792-0
2004-06-01
2024-12-14
Loading full text...

Full text loading...

/deliver/fulltext/jgv/85/6/vir851739.html?itemId=/content/journal/jgv/10.1099/vir.0.79792-0&mimeType=html&fmt=ahah

References

  1. Deom C. M., Wolf S., Holt C. A., Lucas W. J., Beachy R. N. 1991; Altered function of the tobacco mosaic virus movement protein in a hypersensitive host. Virology 180:251–256 [CrossRef]
    [Google Scholar]
  2. Dorokhov Y. L., Skulachev M. V., Ivanov P. A., Zvereva S. D., Tjulkina L. G., Merits A., Gleba Y. Y., Hohn T., Atabekov J. G. 2002; Polypurine (A)-rich sequences promote cross-kingdom conservation of internal ribosome entry. Proc Natl Acad Sci U S A 99:5301–5306 [CrossRef]
    [Google Scholar]
  3. Grdzelishvili V. Z., Chapman S. N., Dawson W. O., Lewandowski D. J. 2000; Mapping of the tobacco mosaic virus movement protein and coat protein subgenomic RNA promoters in vivo. Virology 275:177–192 [CrossRef]
    [Google Scholar]
  4. Guilley H., Jonard G., Kukla B., Richards K. E. 1979; Sequence of 1000 nucleotides at the 3′ end of tobacco mosaic virus RNA. Nucleic Acids Res 6:1287–1308 [CrossRef]
    [Google Scholar]
  5. Hunter T., Jackson R., Zimmern D. 1983; Multiple proteins and subgenomic mRNAs may be derived from a single open reading frame on tobacco mosaic virus RNA. Nucleic Acids Res 11:801–821 [CrossRef]
    [Google Scholar]
  6. Joshi S., Pleij C. W. A., Haenni A. L., Chapeville F., Bosch L. 1983; Properties of the tobacco mosaic virus intermediate RNA-2 and its translation. Virology 127:100–111 [CrossRef]
    [Google Scholar]
  7. Lehto K., Dawson W. O. 1990; Replication, stability, and gene expression of tobacco mosaic virus mutants with a second 30K ORF. Virology 175:30–40 [CrossRef]
    [Google Scholar]
  8. Lehto K., Grantham G. L., Dawson W. O. 1990; Insertion of sequences containing the coat protein subgenomic RNA promoter and leader in front of the tobacco mosaic virus 30K ORF delays its expression and causes defective cell-to-cell movement. Virology 174:145–157 [CrossRef]
    [Google Scholar]
  9. Ooshika I., Watanabe Y., Meshi T., Okada Y., Igano K., Inouye K., Yoshida N. 1984; Identification of the 30K protein of TMV by immunoprecipitation with antibodies directed against a synthetic peptide. Virology 132:71–78 [CrossRef]
    [Google Scholar]
  10. Palukaitis P., Zaitlin M. 1986; Tobacco mosaic virus. Infectivity and replication. In The Plant Virus vol. 2 pp  105–131 Edited by Regenmortel M. H. V., Fraenkel-Conrat M. New York: Plenum;
    [Google Scholar]
  11. Siegel A., Hari V., Kolacz K. 1978; The effect of tobacco mosaic virus infection on host and virus-specific protein synthesis in protoplasts. Virology 85:494–503 [CrossRef]
    [Google Scholar]
  12. Skulachev M. V., Ivanov P. A., Karpova O. V., Korpela T., Rodionova N. P., Dorokhov Y. L., Atabekov J. G. 1999; Internal initiation of translation directed by the 5′-untranslated region of the tobamovirus subgenomic RNA I2 . Virology 263:139–154 [CrossRef]
    [Google Scholar]
  13. Watanabe Y., Meshi T., Okada Y. 1984; The initiation site for transcription of the TMV 30-kDa protein messenger RNA. FEBS Lett 173:247–250 [CrossRef]
    [Google Scholar]
/content/journal/jgv/10.1099/vir.0.79792-0
Loading
/content/journal/jgv/10.1099/vir.0.79792-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error