1887

Abstract

Previously, it has been shown that tobacco mosaic virus (TMV) U1 and crucifer-infecting TMV contain a 75 nt internal ribosome entry site (IRES) upstream of movement protein (MP) gene (IRES and IRES , respectively). A movement-deficient TMV mutant, KK6, has been constructed previously [ Lehto, K., Grantham, G. L. & Dawson, W. O. (1990). , 145–157 ] by insertion of the second coat protein subgenomic promoter (CP SGP-2) upstream of the MP gene, in addition to the natural CP SGP-1. Here, the authors compare the efficiency of movement function expression by KK6 and a derivative, K86, obtained by insertion of IRES between the CP SGP-2 and MP genes resulting in restoration of IRES function in the 5′-untranslated sequence of the I subgenomic RNA of K86. The data indicate that the efficiency of K86 movement was largely restored by this insertion, which was apparently due to the translation-enhancing ability of IRES .

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2004-06-01
2020-01-26
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