1887

Abstract

Marek's disease virus (MDV) is a herpesvirus that causes a lymphoproliferative disease in chickens. Vaccines against MDV are available, but the virus is gradually becoming more virulent. A molecular epidemiology study of MDV was carried out by assessing nucleotide variation in two different genes, and , in 68 French field isolates circulating from 1982 to 1999, compared with reference strains. Viral DNA was amplified by nested PCR and sequenced directly. Comparison of the nucleotide sequences revealed a high nucleotide sequence identity (98 %). Single-nucleotide polymorphisms were identified, leading to the identification of three gene alleles for and six for . Nine combinations of alleles were identified. A majority of French isolates (60·5 %) clustered in the C1 type, which has been present for over 17 years. Waves of non-C1-type isolates appeared when vaccine efficacy decreased. Furthermore, specific discriminating sequences were obtained for the CVI-988 vaccine strain.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.79782-0
2004-06-01
2020-09-30
Loading full text...

Full text loading...

/deliver/fulltext/jgv/85/6/vir851387.html?itemId=/content/journal/jgv/10.1099/vir.0.79782-0&mimeType=html&fmt=ahah

References

  1. Akiyama Y., Kato S., Iwa N. 1973; Continuous cell culture from lymphoma of Marek's disease. Biken J 16:177–179
    [Google Scholar]
  2. Anderson A. S., Parcells M. S., Morgan R. W. 1998; The glycoprotein D (US6) homolog is not essential for oncogenicity or horizontal transmission of Marek's disease virus. J Virol 72:2548–2553
    [Google Scholar]
  3. Calnek B. W., Witter R. L. 1997; Marek's disease. In Diseases of Poultry pp  363–413 Edited by Calnek B. W., Barnes H. J., Beard C. W. Ames, IA: Iowa State University Press;
    [Google Scholar]
  4. Davidson I., Malkinson M., Weisman Y. 2002; Marek's disease in turkeys. I. A seven-year survey of commercial flocks and experimental infection using two field isolates. Avian Dis 46:314–321 [CrossRef]
    [Google Scholar]
  5. Eidson C. S., Schmittle S. C. 1968; Studies on acute Marek's disease. I. Characteristics of isolate GA in chickens. Avian Dis 12:467–476 [CrossRef]
    [Google Scholar]
  6. Faga B., Maury W., Bruckner D. A., Grose C. 2001; Identification and mapping of single nucleotide polymorphisms in the varicella-zoster virus genome. Virology 280:1–6 [CrossRef]
    [Google Scholar]
  7. Fragnet L., Blasco M. A., Klapper W., Rasschaert D. 2003; The RNA subunit of telomerase is encoded by Marek's disease virus. J Virol 77:5985–5996 [CrossRef]
    [Google Scholar]
  8. Franti M., Aubin J. T., Poirel L., Gautheret-Dejean A., Candotti D., Huraux J. M., Agut H. 1998; Definition and distribution analysis of glycoprotein B gene alleles of human herpesvirus 7. J Virol 72:8725–8730
    [Google Scholar]
  9. Kross I., Davis P. J., Shilleto R. W. 1998; Isolation of highly cytolytic MDV strains from Germany and Spain. Avian Pathol 27:313–315 [CrossRef]
    [Google Scholar]
  10. Laurent S., Esnault E., Dambrine G., Goudeau A., Choudat D., Rasschaert D. 2001; Detection of avian oncogenic Marek's disease herpesvirus DNA in human sera. J Gen Virol 82:233–240
    [Google Scholar]
  11. Liu J. L., Kung H. J. 2000; Marek's disease herpesvirus transforming protein MEQ: a c-Jun analogue with an alternative life style. Virus Genes 21:51–64 [CrossRef]
    [Google Scholar]
  12. McGeoch D. J., Dolan A., Ralph A. C. 2000; Toward a comprehensive phylogeny for mammalian and avian herpesviruses. J Virol 74:10401–10406 [CrossRef]
    [Google Scholar]
  13. Oud P. S., Hanselaar T. G., Reubsaet-Veldhuizen J. A., Meijer J. W., Gemmink A. H., Pahlplatz M. M., Beck H. L., Vooijs G. P. 1986; Extraction of nuclei from selected regions in paraffin-embedded tissue. Cytometry 7:595–600 [CrossRef]
    [Google Scholar]
  14. Pradhan H. K., Mohanty G. C., Mukit A. 1985; Marek's disease in Japanese quails ( Coturnix coturnix japonica ): a study of natural cases. Avian Dis 29:575–582 [CrossRef]
    [Google Scholar]
  15. Purchase H. G., Biggs P. M. 1967; Characterization of five isolates of Marek's disease. Res Vet Sci 8:440–449
    [Google Scholar]
  16. Rispens B. H., van Vloten H., Mastenbroek N., Maas H. J., Schat K. A. 1972; Control of Marek's disease in the Netherlands. I. Isolation of an avirulent Marek's disease virus (strain CVI 988) and its use in laboratory vaccination trials. Avian Dis 16:108–125 [CrossRef]
    [Google Scholar]
  17. Schat K. A., Calnek B. W., Fabicant J. 1982; Characterisation of two highly oncogenic strains of Marek's disease virus. Avian Pathol 11:593–605 [CrossRef]
    [Google Scholar]
  18. Voelckel K., Bertram E., Gimeno I., Neumann U., Kaleta E. F. 1999; Evidence for Marek's disease in turkeys in Germany: detection of MDV-1 using the polymerase chain reaction. Acta Virol 43:143–147
    [Google Scholar]
  19. Witter R. L. 1997; Increased virulence of Marek's disease virus field isolates. Avian Dis 41:149–163 [CrossRef]
    [Google Scholar]
  20. Witter R. L., Sharma J. M., Fadly A. M. 1980; Pathogenicity of variant Marek's disease virus isolates in vaccinated and unvaccinated chickens. Avian Dis 24:210–232 [CrossRef]
    [Google Scholar]
  21. Yamaguchi T., Kaplan S. L., Wakenell P., Schat K. A. 2000; Transactivation of latent Marek's disease herpesvirus genes in QT35, a quail fibroblast cell line, by herpesvirus of turkeys. J Virol 74:10176–10186 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.79782-0
Loading
/content/journal/jgv/10.1099/vir.0.79782-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error