1887

Abstract

The SNF2 global transactivator gene homologue () of nucleopolyhedrovirus (BmNPV) is one of the genes exclusive to group I NPVs, but its function remains unknown. This study describes the characterization of . Transcriptional analysis suggested that is an early gene, as its transcript was observed at 4 h post-infection in BmNPV-infected BmN cells. To examine the role of Bm33 during BmNPV infection, a deletion mutant (BmORF33D) was constructed and its infectivity was characterized in BmN cells and larvae. BmORF33D did not have any obvious defects in the production of budded viruses (BVs) or occlusion bodies (OBs) in BmN cells compared with wild-type BmNPV. Larval bioassays revealed that deletion of did not reduce virus infectivity. However, BmORF33D took approximately 10–15 h longer than wild-type BmNPV to kill larvae when tested by either BV injection or OB ingestion. These results suggest that Bm33 is not essential for virus growth or , but that it accelerates the time of death of larvae.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.2008/004887-0
2008-12-01
2019-11-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/89/12/3039.html?itemId=/content/journal/jgv/10.1099/vir.0.2008/004887-0&mimeType=html&fmt=ahah

References

  1. Ayres, M. D., Howard, S. C., Kuzio, J., Lopez-Ferber, M. & Possee, R. D. ( 1994; ). The complete DNA sequence of Autographa californica nuclear polyhedrosis virus. Virology 202, 586–605.[CrossRef]
    [Google Scholar]
  2. Becker, P. B. & Horz, W. ( 2002; ). ATP-dependent nucleosome remodeling. Annu Rev Biochem 71, 247–273.[CrossRef]
    [Google Scholar]
  3. Beniya, H., Braunagel, S. C. & Summers, M. D. ( 1998; ). Autographa californica nuclear polyhedrosis virus: subcellular localization and protein trafficking of BV/ODV-E26 to intranuclear membranes and viral envelopes. Virology 240, 64–75.[CrossRef]
    [Google Scholar]
  4. Federici, B. A. ( 1997; ). Baculovirus pathogenesis. In The Baculoviruses, pp. 33–59. Edited by L. K. Miller. New York: Plenum.
  5. Felsenstein, J. ( 1989; ). phylip – phylogeny inference package (version 3.2). Cladistics 5, 164–166.
    [Google Scholar]
  6. Flaus, A., Martin, D. M., Barton, G. J. & Owen-Hughes, T. ( 2006; ). Identification of multiple distinct Snf2 subfamilies with conserved structural motifs. Nucleic Acids Res 34, 2887–2905.[CrossRef]
    [Google Scholar]
  7. Gomi, S., Majima, K. & Maeda, S. ( 1999; ). Sequence analysis of the genome of Bombyx mori nucleopolyhedrovirus. J Gen Virol 80, 1323–1337.
    [Google Scholar]
  8. Huang, J., Hao, B., Deng, F., Sun, X., Wang, H. & Hu, Z. ( 2008; ). Open reading frame Bm21 of Bombyx mori nucleopolyhedrovirus is not essential for virus replication in vitro, but its deletion extends the median survival time of infected larvae. J Gen Virol 89, 922–930.[CrossRef]
    [Google Scholar]
  9. Hughes, A. L. & Friedman, R. ( 2003; ). Genome-wide survey for genes horizontally transferred from cellular organisms to baculoviruses. Mol Biol Evol 20, 979–987.[CrossRef]
    [Google Scholar]
  10. Ikeda, M., Shikata, M., Shirata, N., Chaeychomsri, S. & Kobayashi, M. ( 2006; ). Gene organization and complete sequence of the Hyphantria cunea nucleopolyhedrovirus genome. J Gen Virol 87, 2549–2562.[CrossRef]
    [Google Scholar]
  11. Imai, N., Matsuda, N., Tanaka, K., Nakano, A., Matsumoto, S. & Kang, W. ( 2003; ). Ubiquitin ligase activities of Bombyx mori nucleopolyhedrovirus RING finger proteins. J Virol 77, 923–930.[CrossRef]
    [Google Scholar]
  12. Iwanaga, M., Takaya, K., Katsuma, S., Ote, M., Tanaka, S., Kamita, S. G., Kang, W., Shimada, T. & Kobayashi, M. ( 2004; ). Expression profiling of baculovirus genes in permissive and nonpermissive cell lines. Biochem Biophys Res Commun 323, 599–614.[CrossRef]
    [Google Scholar]
  13. Jarvis, D. L., Bohlmeyer, D. A. & Garcia, A., Jr ( 1991; ). Requirements for nuclear localization and supramolecular assembly of a baculovirus polyhedrin protein. Virology 185, 795–810.[CrossRef]
    [Google Scholar]
  14. Kamita, S. G., Majima, K. & Maeda, S. ( 1993; ). Identification and characterization of the p35 gene of Bombyx mori nuclear polyhedrosis virus that prevents virus-induced apoptosis. J Virol 67, 455–463.
    [Google Scholar]
  15. Kamita, S. G., Nagasaka, K., Chua, J. W., Shimada, T., Mita, K., Kobayashi, M., Maeda, S. & Hammock, B. D. ( 2005; ). A baculovirus-encoded protein tyrosine phosphatase gene induces enhanced locomotory activity in a lepidopteran host. Proc Natl Acad Sci U S A 102, 2584–2589.[CrossRef]
    [Google Scholar]
  16. Kang, W., Imai, N., Kawasaki, Y., Nagamine, T. & Matsumoto, S. ( 2005; ). IE1 and hr facilitate the localization of Bombyx mori nucleopolyhedrovirus ORF8 to specific nuclear sites. J Gen Virol 86, 3031–3038.[CrossRef]
    [Google Scholar]
  17. Katsuma, S., Shimada, T. & Kobayashi, M. ( 2004; ). Characterization of the baculovirus Bombyx mori nucleopolyhedrovirus gene homologous to the mammalian FGF gene family. Virus Genes 29, 211–217.[CrossRef]
    [Google Scholar]
  18. Katsuma, S., Horie, S., Daimon, T., Iwanaga, M. & Shimada, T. ( 2006; ). In vivo and in vitro analyses of a Bombyx mori nucleopolyhedrovirus mutant lacking functional vfgf. Virology 355, 62–70.[CrossRef]
    [Google Scholar]
  19. Katsuma, S., Mita, K. & Shimada, T. ( 2007; ). ERK- and JNK-dependent signaling pathways contribute to Bombyx mori nucleopolyhedrovirus infection. J Virol 81, 13700–13709.[CrossRef]
    [Google Scholar]
  20. Kumar, R. A., Naidu, S. R., Wang, X., Imbalzano, A. N. & Androphy, E. J. ( 2007; ). Interaction of papillomavirus E2 protein with the Brm chromatin remodeling complex leads to enhanced transcriptional activation. J Virol 81, 2213–2220.[CrossRef]
    [Google Scholar]
  21. Lapointe, R., Back, D. W., Ding, Q. & Carstens, E. B. ( 2000; ). Identification and molecular characterization of the Choristoneura fumiferana multicapsid nucleopolyhedrovirus genomic region encoding the regulatory genes pkip, p47, lef-12, and gta. Virology 271, 109–121.[CrossRef]
    [Google Scholar]
  22. Li, G., Wang, J., Deng, R. & Wang, X. ( 2008; ). Characterization of AcMNPV with a deletion of ac68 gene. Virus Genes 37, 119–127.[CrossRef]
    [Google Scholar]
  23. Lung, O. Y., Cruz-Alvarez, M. & Blissard, G. W. ( 2003; ). Ac23, an envelope fusion protein homolog in the baculovirus Autographa californica multicapsid nucleopolyhedrovirus, is a viral pathogenicity factor. J Virol 77, 328–339.[CrossRef]
    [Google Scholar]
  24. Maeda, S. & Majima, K. ( 1990; ). Molecular cloning and physical mapping of the genome of Bombyx mori nuclear polyhedrosis virus. J Gen Virol 71, 1851–1855.[CrossRef]
    [Google Scholar]
  25. Mahmoudi, T., Parra, M., Vries, R. G., Kauder, S. E., Verrijzer, C. P., Ott, M. & Verdin, E. ( 2006; ). The SWI/SNF chromatin-remodeling complex is a cofactor for Tat transactivation of the HIV promoter. J Biol Chem 281, 19960–19968.[CrossRef]
    [Google Scholar]
  26. Monsma, S. A., Oomens, A. G. & Blissard, G. W. ( 1996; ). The GP64 envelope fusion protein is an essential baculovirus protein required for cell-to-cell transmission of infection. J Virol 70, 4607–4616.
    [Google Scholar]
  27. Prikhod'ko, E. A. & Miller, L. K. ( 1998; ). Role of baculovirus IE2 and its RING finger in cell cycle arrest. J Virol 72, 684–692.
    [Google Scholar]
  28. Pullen, S. S. & Friesen, P. D. ( 1995; ). The CAGT motif functions as an initiator element during early transcription of the baculovirus transregulator ie-1. J Virol 69, 3575–3583.
    [Google Scholar]
  29. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  30. Xu, H. J., Yang, Z. N., Zhao, J. F., Tian, C. H., Ge, J. Q., Tang, X. D., Bao, Y. Y. & Zhang, C. X. ( 2008; ). Bombyx mori nucleopolyhedrovirus ORF56 encodes an occlusion-derived virus protein and is not essential for budded virus production. J Gen Virol 89, 1212–1219.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.2008/004887-0
Loading
/content/journal/jgv/10.1099/vir.0.2008/004887-0
Loading

Data & Media loading...

Supplements

vol. , part 12, pp. 3039 - 3046

List of primers [PDF](42 KB)



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error