RT Journal Article SR Electronic(1) A1 Coelho, Fabiana Magalhães A1 Bomfim, Maria Rosa Quaresma A1 de Andrade Caxito, Fabíola A1 Ribeiro, Natália Almeida A1 Luppi, Marcela Miranda A1 Costa, Érica Azevedo A1 Oliveira, Maria Emilia A1 Da Fonseca, Flávio Guimarães A1 Resende, MauricioYR 2008 T1 Naturally occurring feline leukemia virus subgroup A and B infections in urban domestic cats JF Journal of General Virology, VO 89 IS 11 SP 2799 OP 2805 DO https://doi.org/10.1099/vir.0.2008/003855-0 PB Microbiology Society, SN 1465-2099, AB A nested-PCR (n-PCR) was used to detect feline leukemia virus (FeLV) proviral DNA in blood samples from 464 sick and 608 healthy domestic cats (Felis catus) selected by convenience, and a significantly high prevalence of FeLV infection was observed. n-PCR results revealed the presence of FeLV proviral DNA in 47.2 % of sick cats and 47.4 % of healthy cats. Phylogenetic analysis revealed that FeLV samples from healthy or sick cats were grouped into separate clades. We determined FeLV subgroups by an n-PCR based on the envelope (env) gene. The partial env gene of FeLV Minas Gerais (MG) samples were compared to various exogenous FeLV isolates and endogenous (enFeLV) provirus from the same region. FeLV-B MG samples were more similar to endogenous sequences and to natural FeLV-B isolates than to either FeLV-A or FeLV-C. The results revealed the circulation of FeLV-B in large populations of urban domestic cats in Brazil., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.2008/003855-0