In 2003, we described the first human G6P[6] rotavirus strain (B1711). To investigate further the molecular origin of this strain and to determine the possible reassortments leading to this new gene constellation, the complete genome of strain B1711 was sequenced. SimPlot analyses were conducted to compare strain B1711 with other known rotavirus gene segments, and phylogenetic dendrograms were constructed to analyse the origin of the eleven genome segments of strain B1711. Our analysis indicated that strain B1711 acquired its VP1-, VP2-, VP4-, VP6- and NSP1–5-encoding gene segments from human DS-1-like P[6] rotavirus strains, and its VP3 and VP7 gene segments from a bovine rotavirus strain through reassortment. The introduction of animal–human reassortant strains, which might arise in either of the hosts, into the human rotavirus population is an important mechanism for the generation of rotavirus diversity, and might be a challenge for the current rotavirus vaccines and vaccines under development.
BányaiK.,
GentschJ. R.,
GriffinD. D.,
HolmesJ. L.,
GlassR. I.,
SzucsG.2003; Genetic variability among serotype G6 human rotaviruses: identification of a novel lineage isolated in Hungary. J Med Virol 71:124–134[CrossRef]
EstesM.,
KapikianA.2007; Rotaviruses. In Fields Virology , 5th edn. vol 2 pp 1917–1974Edited byKnipeD. M.,
HowleyP. M.,
GriffinD. E.,
LambR. A.,
MartinM. A.,
RoizmanB.,
StrausS. E.
Philadelphia, PA: Kluwer/Lippincott, Williams and Wilkins;
GernaG.,
SarasiniA.,
PareaM.,
AristaS.,
MirandaP.,
BrussowH.,
HoshinoY.,
FloresJ.1992; Isolation and characterization of two distinct human rotavirus strains with G6 specificity. J Clin Microbiol 30:9–16
GriffinD. D.,
NakagomiT.,
HoshinoY.,
NakagomiO.,
KirkwoodC. D.,
ParasharU. D.,
GlassR. I.,
GentschJ. R.2002; Characterization of nontypeable rotavirus strains from the United States: identification of a new rotavirus reassortant (P2A[6],G12) and rare P3[9] strains related to bovine rotaviruses. Virology 294:256–269[CrossRef]
HoshinoY.,
SaifL. J.,
KangS. Y.,
SerenoM. M.,
ChenW. K.,
KapikianA. Z.1995; Identification of group A rotavirus genes associated with virulence of a porcine rotavirus and host range restriction of a human rotavirus in the gnotobiotic piglet model. Virology 209:274–280[CrossRef]
ItoH.,
SugiyamaM.,
MasubuchiK.,
MoriY.,
MinamotoN.2001; Complete nucleotide sequence of a group A avian rotavirus genome and a comparison with its counterparts of mammalian rotaviruses. Virus Res 75:123–138[CrossRef]
MatthijnssensJ.,
RahmanM.,
MartellaV.,
XueleiY.,
De VosS.,
De LeenerK.,
CiarletM.,
BuonavogliaC.,
Van RanstM.2006a; Full genomic analysis of human rotavirus strain B4106 and lapine rotavirus strain 30/96 provides evidence for interspecies transmission. J Virol 80:3801–3810[CrossRef]
MatthijnssensJ.,
RahmanM.,
YangX.,
DelbekeT.,
ArijsI.,
KabueJ. P.,
MuyembeJ. J.,
Van RanstM.2006b; G8 rotavirus strains isolated in the Democratic Republic of Congo belong to the DS-1-like genogroup. J Clin Microbiol 44:1801–1809[CrossRef]
MatthijnssensJ.,
CiarletM.,
HeimanE.,
ArijsI.,
DelbekeT.,
McDonaldS. M.,
PalomboA. E.,
Iturriza-GómaraM.,
MaesP.other authors2008a; Full genome-based classification of rotaviruses reveals common origin between human Wa-like and porcine rotavirus strains and human DS-1-like and bovine rotavirus strains. J Virol 82:3204–3219[CrossRef]
MatthijnssensJ.,
RahmanM.,
CiarletM.,
Van RanstM.2008b; Emerging human rotavirus genotypes. In Viruses in the Environment pp 171–219Edited byPalomboA. E.,
KirkwoodC. D.
Trivandrum, India: Research Signpost;
PalomboE. A.,
BishopR. F.1995; Genetic and antigenic characterization of a serotype G6 human rotavirus isolated in Melbourne, Australia. J Med Virol 47:348–354[CrossRef]
RahmanM.,
De LeenerK.,
GoegebuerT.,
WollantsE.,
van der DonckI.,
Van HoovelsL.,
Van RanstM.2003; Genetic characterization of a novel, naturally occurring recombinant human G6P[6] rotavirus. J Clin Microbiol 41:2088–2095[CrossRef]
RahmanM.,
MatthijnssensJ.,
NaharS.,
PodderG.,
SackD. A.,
AzimT.,
Van RanstM.2005; Characterization of a novel P[25],G11 human group a rotavirus. J Clin Microbiol 43:3208–3212[CrossRef]
RahmanM.,
MatthijnssensJ.,
YangX.,
DelbekeT.,
ArijsI.,
TaniguchiK.,
Iturriza-GómaraM.,
IftekharuddinN.,
AzimT.,
Van RanstM.2007; Evolutionary history and global spread of the emerging G12 human rotaviruses. J Virol 81:2382–2390[CrossRef]
RaoC. D.,
GowdaK.,
ReddyB. S.2000; Sequence analysis of VP4 and VP7 genes of nontypeable strains identifies a new pair of outer capsid proteins representing novel P and G genotypes in bovine rotaviruses. Virology 276:104–113[CrossRef]
SantosN.,
HoshinoY.2005; Global distribution of rotavirus serotypes/genotypes and its implication for the development and implementation of an effective rotavirus vaccine. Rev Med Virol 15:29–56[CrossRef]
SmallC.,
BarroM.,
BrownT. L.,
PattonJ. T.2007; Genome heterogeneity of SA11 rotavirus due to reassortment with “O” agent. Virology 359:415–424[CrossRef]
SteyerA.,
Poljsak-PrijateljM.,
Barlic-MaganjaD.,
JamnikarU.,
MijovskiJ. Z.,
MarinJ.2007; Molecular characterization of a new porcine rotavirus P genotype found in an asymptomatic pig in Slovenia. Virology 359:275–282[CrossRef]
ThompsonJ. D.,
GibsonT. J.,
PlewniakF.,
JeanmouginF.,
HigginsD. G.1997; The clustal_x Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882[CrossRef]
Van DammeP.,
GiaquintoC.,
MaxwellM.,
ToddP.,
Van der WielenM.2007; Distribution of rotavirus genotypes in Europe, 2004–2005: the REVEAL Study. J Infect Dis 195:Suppl 1S17–S25[CrossRef]
VesikariT.,
KarvonenA.,
PrymulaR.,
SchusterV.,
TejedorJ. C.,
CohenR.,
MeuriceF.,
HanH. H.,
DamasoS.,
BouckenoogheA.2007; Efficacy of human rotavirus vaccine against rotavirus gastroenteritis during the first 2 years of life in European infants: randomised, double-blind controlled study. Lancet 370:1757–1763[CrossRef]