1887

Abstract

Recombinant pestivirus envelope glycoprotein E has been shown to interfere with dsRNA-induced interferon (IFN-/) synthesis. This study demonstrated that authentic, enzymically active E produced in mammalian cells prevented a dsRNA-induced IFN response when present in the supernatant of bovine cells. Strikingly, IFN synthesis of cells expressing E was eliminated after extracellular addition, but not transfection, of dsRNA. Importantly, the same applied to cells infected with bovine viral diarrhea virus (BVDV) expressing E but lacking the N-terminal protease N. Free E concentrations circulating in the blood of animals persistently infected with BVDV were determined to be approximately 50 ng ml, i.e. at a similar order of magnitude as that displaying an effect on dsRNA-induced IFN expression . Whilst N blocks interferon regulatory factor-3-dependent IFN induction in infected cells, E may prevent constant IFN induction in uninfected cells by dsRNA that could originate from pestivirus-infected cells. This probably contributes to the survival of persistently BVDV-infected animals and maintains viral persistence in the host population.

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2008-10-01
2020-01-29
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