Inhibition of duck hepatitis B virus infection of liver cells by combined treatment with viral e antigen and carbohydrates Free

Abstract

The e antigen (eAg) of duck hepatitis B virus (DHBV) is a glycosylated secretory protein with a currently unknown function. We concentrated this antigen from the supernatants of persistently infected primary duck liver cell cultures by ammonium sulphate precipitation, adsorption chromatography over concanavalin A Sepharose, preparative isoelectric focusing and molecular sieve chromatography. The combined treatment of duck liver cells with DHBV eAg (DHBe) concentrate and -methyl--mannopyranoside strongly inhibited DHBV replication at infection. When DHBe was added to non-infected primary duck liver cells, it was found to be associated with liver sinusoidal endothelial cells. This binding could be inhibited by the addition of -methyl--mannopyranoside and other sugar molecules. The inhibitory effect of DHBe on infection could play a role in maintaining viral persistence.

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2008-12-01
2024-03-28
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References

  1. Bradford M. M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254 [CrossRef]
    [Google Scholar]
  2. Breiner K. M., Urban S., Schaller H. 1998; Carboxypeptidase D (gp180), a Golgi-resident protein, functions in the attachment and entry of avian hepatitis B viruses. J Virol 72:8098–8104
    [Google Scholar]
  3. Breiner K. M., Schaller H., Knolle P. A. 2001; Endothelial cell-mediated uptake of a hepatitis B virus: a new concept of liver targeting of hepatotropic microorganisms. Hepatology 34:803–808 [CrossRef]
    [Google Scholar]
  4. Bruns M., Frenzel B. 1979; Isolation of a glycoprotein and two structural proteins of Maedi-Visna virus. Virology 97:207–211 [CrossRef]
    [Google Scholar]
  5. Bruns M., Maenz C. 2007; Requirement of activation for hepatitis B virus infection. Available from Nature Precedings http://hdl.handle.net/10101/npre.2007.221.1
  6. Bruns M., Miska S., Chassot S., Will H. 1998; Enhancement of hepatitis B virus infection by noninfectious subviral particles. J Virol 72:1462–1468
    [Google Scholar]
  7. Buscher M., Reiser W., Will H., Schaller H. 1985; Transcripts and the putative RNA pregenome of duck hepatitis B virus: implications for reverse transcription. Cell 40:717–724 [CrossRef]
    [Google Scholar]
  8. Chang C., Enders G., Sprengel R., Peters N., Varmus H. E., Ganem D. 1987; Expression of the precore region of an avian hepatitis B virus is not required for viral replication. J Virol 61:3322–3325
    [Google Scholar]
  9. Cohen B. J., Richmond J. E. 1982; Electron microscopy of hepatitis B core antigen synthesized in E. coli . Nature 296:677–679 [CrossRef]
    [Google Scholar]
  10. Edwards S. A., Fan H. 1980; Sequence relationship of glycosylated and unglycosylated gag polyproteins of Moloney murine leukemia virus. J Virol 35:41–51
    [Google Scholar]
  11. Enders G. H., Ganem D., Varmus H. 1985; Mapping the major transcripts of ground squirrel hepatitis virus: the presumptive template for reverse transcriptase is terminally redundant. Cell 42:297–308 [CrossRef]
    [Google Scholar]
  12. Fernholz D., Wildner G., Will H. 1993; Minor envelope proteins of duck hepatitis B virus are initiated at internal pre-S AUG codons but are not essential for infectivity. Virology 197:64–73 [CrossRef]
    [Google Scholar]
  13. Franke C., Matschl U., Bruns M. 2007; Enzymatic treatment of duck hepatitis B virus: topology of the surface proteins for virions and noninfectious subviral particles. Virology 359:126–137 [CrossRef]
    [Google Scholar]
  14. Götz W., Reinauer H., Rosenbauer K. A. 1990 Lebererkrankungen, Labordiagnostik und Epidemiologie Darmstadt: GIT Verlag;
    [Google Scholar]
  15. Irie S., Tavassoli M. 1986; Liver endothelium desialates ceruloplasmin. Biochem Biophys Res Commun 140:94–100 [CrossRef]
    [Google Scholar]
  16. Irving M. G., Roll F. J., Huang S., Bissell D. M. 1984; Characterization and culture of sinusoidal endothelium from normal rat liver: lipoprotein uptake and collagen phenotype. Gastroenterology 87:1233–1247
    [Google Scholar]
  17. Jilbert A. R., Wu T. T., England J. M., Hall P. M., Carp N. Z., O'Connell A. P., Mason W. S. 1992; Rapid resolution of duck hepatitis B virus infections occurs after massive hepatocellular involvement. J Virol 66:1377–1388
    [Google Scholar]
  18. Kawaguchi T., Nomura K., Hirayama Y., Kitagawa T. 1987; Establishment and characterization of a chicken hepatocellular carcinoma cell line, LMH. Cancer Res 47:4460–4464
    [Google Scholar]
  19. Knolle P. A., Ohl J., Limmer A. 2000; Unique regulation of the hepatic immune response by a new antigen-presenting cell type: the sinusoidal endothelial cell of the liver. In Chronic Hepatitis: New Concepts of Pathogenesis, Diagnosis and Treatment pp 74–91Edited by Dienes H., Schirmacher P., Brechot C., Okuda. Köln: Kluwer;
    [Google Scholar]
  20. Kuroki K., Cheung R., Marion P. L., Ganem D. 1994; A cell surface protein that binds avian hepatitis B virus particles. J Virol 68:2091–2096
    [Google Scholar]
  21. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685 [CrossRef]
    [Google Scholar]
  22. Maenz C., Chang S. F., Iwanski A., Bruns M. 2007; Entry of duck hepatitis B virus into primary duck liver and kidney cells after discovery of a fusogenic region within the large surface protein. J Virol 81:5014–5023 [CrossRef]
    [Google Scholar]
  23. Milich D., Liang T. J. 2003; Exploring the biological basis of hepatitis B e antigen in hepatitis B virus infection. Hepatology 38:1075–1086 [CrossRef]
    [Google Scholar]
  24. Milich D. R., Wolf S. F., Hughes J. L., Jones J. E. 1995; Interleukin 12 suppresses autoantibody production by reversing helper T-cell phenotype in hepatitis B e antigen transgenic mice. Proc Natl Acad Sci U S A 92:6847–6851 [CrossRef]
    [Google Scholar]
  25. Milich D. R., Chen M. K., Hughes J. L., Jones J. E. 1998; The secreted hepatitis B precore antigen can modulate the immune response to the nucleocapsid: a mechanism for persistence. J Immunol 160:2013–2021
    [Google Scholar]
  26. Moroy T., Etiemble J., Trepo C., Tiollais P., Buendia M. A. 1985; Transcription of woodchuck hepatitis virus in the chronically infected liver. EMBO J 4:1507–1514
    [Google Scholar]
  27. Netter H.-J., Chang S.-F., Bruns M. 2008; Host-range and pathogenicity of hepatitis B viruses. Future Virol 3:83–94 [CrossRef]
    [Google Scholar]
  28. Okaji Y., Tsuno N. H., Kitayama J., Saito S., Takahashi T., Kawai K., Yazawa K., Asakage M., Tsuchiya T. other authors 2004; A novel method for isolation of endothelial cells and macrophages from murine tumors based on ac-LDL uptake and CD16 expression. J Immunol Methods 295:183–193 [CrossRef]
    [Google Scholar]
  29. Ou J. H., Laub O., Rutter W. J. 1986; Hepatitis B virus gene function: the precore region targets the core antigen to cellular membranes and causes the secretion of the e antigen. Proc Natl Acad Sci U S A 83:1578–1582 [CrossRef]
    [Google Scholar]
  30. Schlicht H. J. 1991; Biosynthesis of the secretory core protein of duck hepatitis B virus: intracellular transport, proteolytic processing, and membrane expression of the precore protein. J Virol 65:3489–3495
    [Google Scholar]
  31. Schlicht H. J., Schaller H. 1989; The secretory core protein of human hepatitis B virus is expressed on the cell surface. J Virol 63:5399–5404
    [Google Scholar]
  32. Schlicht H. J., Salfeld J., Schaller H. 1987; The duck hepatitis B virus pre-C region encodes a signal sequence which is essential for synthesis and secretion of processed core proteins but not for virus formation. J Virol 61:3701–3709
    [Google Scholar]
  33. Schlicht H. J., Wasenauer G., Kock J. 1993; Molecular basis of the diversity of hepatitis B virus core-gene products. Arch Virol Suppl 8:43–52
    [Google Scholar]
  34. Schneider R., Fernholz D., Wildner G., Will H. 1991; Mechanism, kinetics, and role of duck hepatitis B virus e-antigen expression in vivo. Virology 182:503–512 [CrossRef]
    [Google Scholar]
  35. Schwartzberg P., Colicelli J., Goff S. P. 1983; Deletion mutants of Moloney murine leukemia virus which lack glycosylated gag protein are replication competent. J Virol 46:538–546
    [Google Scholar]
  36. Takahashi K., Imai M., Miyakawa Y., Iwakiri S., Mayumi M. 1978; Duality of hepatitis B e antigen in serum of persons infected with hepatitis B virus: evidence for the nonidentity of e antigen with immunoglobulins. Proc Natl Acad Sci U S A 75:1952–1956 [CrossRef]
    [Google Scholar]
  37. Tavassoli M., Kishimoto T., Soda R., Kataoka M., Harjes K. 1986; Liver endothelium mediates the uptake of iron-transferrin complex by hepatocytes. Exp Cell Res 165:369–379 [CrossRef]
    [Google Scholar]
  38. Thio C. L., Mosbruger T., Astemborski J., Greer S., Kirk G. D., O'Brien S. J., Thomas D. L. 2005; Mannose binding lectin genotypes influence recovery from hepatitis B virus infection. J Virol 79:9192–9196 [CrossRef]
    [Google Scholar]
  39. Tiollais P., Pourcel C., Dejean A. 1985; The hepatitis B virus. Nature 317:489–495 [CrossRef]
    [Google Scholar]
  40. Urban S., Schwarz C., Marx U. C., Zentgraf H., Schaller H., Multhaup G. 2000; Receptor recognition by a hepatitis B virus reveals a novel mode of high affinity virus-receptor interaction. EMBO J 19:1217–1227 [CrossRef]
    [Google Scholar]
  41. Zhang Y. Y., Summers J. 1999; Enrichment of a precore-minus mutant of duck hepatitis B virus in experimental mixed infections. J Virol 73:3616–3622
    [Google Scholar]
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