RT Journal Article SR Electronic(1) A1 Maenz, Claudia A1 Loscher, Christine A1 Iwanski, Alicja A1 Bruns, MichaelYR 2008 T1 Inhibition of duck hepatitis B virus infection of liver cells by combined treatment with viral e antigen and carbohydrates JF Journal of General Virology, VO 89 IS 12 SP 3016 OP 3026 DO https://doi.org/10.1099/vir.0.2008/003541-0 PB Microbiology Society, SN 1465-2099, AB The e antigen (eAg) of duck hepatitis B virus (DHBV) is a glycosylated secretory protein with a currently unknown function. We concentrated this antigen from the supernatants of persistently infected primary duck liver cell cultures by ammonium sulphate precipitation, adsorption chromatography over concanavalin A Sepharose, preparative isoelectric focusing and molecular sieve chromatography. The combined treatment of duck liver cells with DHBV eAg (DHBe) concentrate and α-methyl-d-mannopyranoside strongly inhibited DHBV replication at de novo infection. When DHBe was added to non-infected primary duck liver cells, it was found to be associated with liver sinusoidal endothelial cells. This binding could be inhibited by the addition of α-methyl-d-mannopyranoside and other sugar molecules. The inhibitory effect of DHBe on infection could play a role in maintaining viral persistence., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.2008/003541-0